A catalog of human cDNA expression clones and its application to structural genomics

We describe here a systematic approach to the identification of human proteins and protein fragments that can be expressed as soluble proteins in Escherichia coli. A cDNA expression library of 10,825 clones was screened by small-scale expression and purification and 2,746 clones were identified. Seq...

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Detalles Bibliográficos
Autores principales: Büssow, Konrad, Quedenau, Claudia, Sievert, Volker, Tischer, Janett, Scheich, Christoph, Seitz, Harald, Hieke, Brigitte, Niesen, Frank H, Götz, Frank, Harttig, Ulrich, Lehrach, Hans
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2004
Materias:
Method
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC522878/
https://www.ncbi.nlm.nih.gov/pubmed/15345055
http://dx.doi.org/10.1186/gb-2004-5-9-r71
Descripción
Sumario:We describe here a systematic approach to the identification of human proteins and protein fragments that can be expressed as soluble proteins in Escherichia coli. A cDNA expression library of 10,825 clones was screened by small-scale expression and purification and 2,746 clones were identified. Sequence and protein-expression data were entered into a public database. A set of 163 clones was selected for structural analysis and 17 proteins were prepared for crystallization, leading to three new structures.

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