Large-scale exploration of growth inhibition caused by overexpression of genomic fragments in Saccharomyces cerevisiae

We have screened the genome of Saccharomyces cerevisiae for fragments that confer a growth-retardation phenotype when overexpressed in a multicopy plasmid with a tetracycline-regulatable (Tet-off) promoter. We selected 714 such fragments with a mean size of 700 base-pairs out of around 84,000 clones...

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Detalles Bibliográficos
Autores principales: Boyer, Jeanne, Badis, Gwenaël, Fairhead, Cécile, Talla, Emmanuel, Hantraye, Florence, Fabre, Emmanuelle, Fischer, Gilles, Hennequin, Christophe, Koszul, Romain, Lafontaine, Ingrid, Ozier-Kalogeropoulos, Odile, Ricchetti, Miria, Richard, Guy-Franck, Thierry, Agnès, Dujon, Bernard
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2004
Materias:
Method
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC522879/
https://www.ncbi.nlm.nih.gov/pubmed/15345056
http://dx.doi.org/10.1186/gb-2004-5-9-r72
Descripción
Sumario:We have screened the genome of Saccharomyces cerevisiae for fragments that confer a growth-retardation phenotype when overexpressed in a multicopy plasmid with a tetracycline-regulatable (Tet-off) promoter. We selected 714 such fragments with a mean size of 700 base-pairs out of around 84,000 clones tested. These include 493 in-frame open reading frame fragments corresponding to 454 distinct genes (of which 91 are of unknown function), and 162 out-of-frame, antisense and intergenic genomic fragments, representing the largest collection of toxic inserts published so far in yeast.

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