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Large-scale exploration of growth inhibition caused by overexpression of genomic fragments in Saccharomyces cerevisiae
We have screened the genome of Saccharomyces cerevisiae for fragments that confer a growth-retardation phenotype when overexpressed in a multicopy plasmid with a tetracycline-regulatable (Tet-off) promoter. We selected 714 such fragments with a mean size of 700 base-pairs out of around 84,000 clones...
| Autores principales: | , , , , , , , , , , , , , , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
BioMed Central
2004
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC522879/ https://www.ncbi.nlm.nih.gov/pubmed/15345056 http://dx.doi.org/10.1186/gb-2004-5-9-r72 |
| _version_ | 1782121868408389632 |
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| author | Boyer, Jeanne Badis, Gwenaël Fairhead, Cécile Talla, Emmanuel Hantraye, Florence Fabre, Emmanuelle Fischer, Gilles Hennequin, Christophe Koszul, Romain Lafontaine, Ingrid Ozier-Kalogeropoulos, Odile Ricchetti, Miria Richard, Guy-Franck Thierry, Agnès Dujon, Bernard |
| author_facet | Boyer, Jeanne Badis, Gwenaël Fairhead, Cécile Talla, Emmanuel Hantraye, Florence Fabre, Emmanuelle Fischer, Gilles Hennequin, Christophe Koszul, Romain Lafontaine, Ingrid Ozier-Kalogeropoulos, Odile Ricchetti, Miria Richard, Guy-Franck Thierry, Agnès Dujon, Bernard |
| author_sort | Boyer, Jeanne |
| collection | PubMed |
| description | We have screened the genome of Saccharomyces cerevisiae for fragments that confer a growth-retardation phenotype when overexpressed in a multicopy plasmid with a tetracycline-regulatable (Tet-off) promoter. We selected 714 such fragments with a mean size of 700 base-pairs out of around 84,000 clones tested. These include 493 in-frame open reading frame fragments corresponding to 454 distinct genes (of which 91 are of unknown function), and 162 out-of-frame, antisense and intergenic genomic fragments, representing the largest collection of toxic inserts published so far in yeast. |
| format | Text |
| id | pubmed-522879 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2004 |
| publisher | BioMed Central |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-5228792004-10-17 Large-scale exploration of growth inhibition caused by overexpression of genomic fragments in Saccharomyces cerevisiae Boyer, Jeanne Badis, Gwenaël Fairhead, Cécile Talla, Emmanuel Hantraye, Florence Fabre, Emmanuelle Fischer, Gilles Hennequin, Christophe Koszul, Romain Lafontaine, Ingrid Ozier-Kalogeropoulos, Odile Ricchetti, Miria Richard, Guy-Franck Thierry, Agnès Dujon, Bernard Genome Biol Method We have screened the genome of Saccharomyces cerevisiae for fragments that confer a growth-retardation phenotype when overexpressed in a multicopy plasmid with a tetracycline-regulatable (Tet-off) promoter. We selected 714 such fragments with a mean size of 700 base-pairs out of around 84,000 clones tested. These include 493 in-frame open reading frame fragments corresponding to 454 distinct genes (of which 91 are of unknown function), and 162 out-of-frame, antisense and intergenic genomic fragments, representing the largest collection of toxic inserts published so far in yeast. BioMed Central 2004 2004-08-31 /pmc/articles/PMC522879/ /pubmed/15345056 http://dx.doi.org/10.1186/gb-2004-5-9-r72 Text en Copyright © 2004 Boyer et al.; licensee BioMed Central Ltd. |
| spellingShingle | Method Boyer, Jeanne Badis, Gwenaël Fairhead, Cécile Talla, Emmanuel Hantraye, Florence Fabre, Emmanuelle Fischer, Gilles Hennequin, Christophe Koszul, Romain Lafontaine, Ingrid Ozier-Kalogeropoulos, Odile Ricchetti, Miria Richard, Guy-Franck Thierry, Agnès Dujon, Bernard Large-scale exploration of growth inhibition caused by overexpression of genomic fragments in Saccharomyces cerevisiae |
| title | Large-scale exploration of growth inhibition caused by overexpression of genomic fragments in Saccharomyces cerevisiae |
| title_full | Large-scale exploration of growth inhibition caused by overexpression of genomic fragments in Saccharomyces cerevisiae |
| title_fullStr | Large-scale exploration of growth inhibition caused by overexpression of genomic fragments in Saccharomyces cerevisiae |
| title_full_unstemmed | Large-scale exploration of growth inhibition caused by overexpression of genomic fragments in Saccharomyces cerevisiae |
| title_short | Large-scale exploration of growth inhibition caused by overexpression of genomic fragments in Saccharomyces cerevisiae |
| title_sort | large-scale exploration of growth inhibition caused by overexpression of genomic fragments in saccharomyces cerevisiae |
| topic | Method |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC522879/ https://www.ncbi.nlm.nih.gov/pubmed/15345056 http://dx.doi.org/10.1186/gb-2004-5-9-r72 |
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