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Bacterial Communities Associated with Houseflies (Musca domestica L.) Sampled within and between Farms
The housefly feeds and reproduces in animal manure and decaying organic substances and thus lives in intimate association with various microorganisms including human pathogens. In order to understand the variation and association between bacteria and the housefly, we used 16S rRNA gene amplicon sequ...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5232358/ https://www.ncbi.nlm.nih.gov/pubmed/28081167 http://dx.doi.org/10.1371/journal.pone.0169753 |
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author | Bahrndorff, Simon de Jonge, Nadieh Skovgård, Henrik Nielsen, Jeppe Lund |
author_facet | Bahrndorff, Simon de Jonge, Nadieh Skovgård, Henrik Nielsen, Jeppe Lund |
author_sort | Bahrndorff, Simon |
collection | PubMed |
description | The housefly feeds and reproduces in animal manure and decaying organic substances and thus lives in intimate association with various microorganisms including human pathogens. In order to understand the variation and association between bacteria and the housefly, we used 16S rRNA gene amplicon sequencing to describe bacterial communities of 90 individual houseflies collected within and between ten dairy farms in Denmark. Analysis of gene sequences showed that the most abundant classes of bacteria found across all sites included Bacilli, Clostridia, Actinobacteria, Flavobacteria, and all classes of Proteobacteria and at the genus level the most abundant genera included Corynebacterium, Lactobacillus, Staphylococcus, Vagococcus, Weissella, Lactococcus, and Aerococcus. Comparison of the microbiota of houseflies revealed a highly diverse microbiota compared to other insect species and with most variation in species richness and diversity found between individuals, but not locations. Our study is the first in-depth amplicon sequencing study of the housefly microbiota, and collectively shows that the microbiota of single houseflies is highly diverse and differs between individuals likely to reflect the lifestyle of the housefly. We suggest that these results should be taken into account when addressing the transmission of pathogens by the housefly and assessing the vector competence variation under natural conditions. |
format | Online Article Text |
id | pubmed-5232358 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-52323582017-01-31 Bacterial Communities Associated with Houseflies (Musca domestica L.) Sampled within and between Farms Bahrndorff, Simon de Jonge, Nadieh Skovgård, Henrik Nielsen, Jeppe Lund PLoS One Research Article The housefly feeds and reproduces in animal manure and decaying organic substances and thus lives in intimate association with various microorganisms including human pathogens. In order to understand the variation and association between bacteria and the housefly, we used 16S rRNA gene amplicon sequencing to describe bacterial communities of 90 individual houseflies collected within and between ten dairy farms in Denmark. Analysis of gene sequences showed that the most abundant classes of bacteria found across all sites included Bacilli, Clostridia, Actinobacteria, Flavobacteria, and all classes of Proteobacteria and at the genus level the most abundant genera included Corynebacterium, Lactobacillus, Staphylococcus, Vagococcus, Weissella, Lactococcus, and Aerococcus. Comparison of the microbiota of houseflies revealed a highly diverse microbiota compared to other insect species and with most variation in species richness and diversity found between individuals, but not locations. Our study is the first in-depth amplicon sequencing study of the housefly microbiota, and collectively shows that the microbiota of single houseflies is highly diverse and differs between individuals likely to reflect the lifestyle of the housefly. We suggest that these results should be taken into account when addressing the transmission of pathogens by the housefly and assessing the vector competence variation under natural conditions. Public Library of Science 2017-01-12 /pmc/articles/PMC5232358/ /pubmed/28081167 http://dx.doi.org/10.1371/journal.pone.0169753 Text en © 2017 Bahrndorff et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Bahrndorff, Simon de Jonge, Nadieh Skovgård, Henrik Nielsen, Jeppe Lund Bacterial Communities Associated with Houseflies (Musca domestica L.) Sampled within and between Farms |
title | Bacterial Communities Associated with Houseflies (Musca domestica L.) Sampled within and between Farms |
title_full | Bacterial Communities Associated with Houseflies (Musca domestica L.) Sampled within and between Farms |
title_fullStr | Bacterial Communities Associated with Houseflies (Musca domestica L.) Sampled within and between Farms |
title_full_unstemmed | Bacterial Communities Associated with Houseflies (Musca domestica L.) Sampled within and between Farms |
title_short | Bacterial Communities Associated with Houseflies (Musca domestica L.) Sampled within and between Farms |
title_sort | bacterial communities associated with houseflies (musca domestica l.) sampled within and between farms |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5232358/ https://www.ncbi.nlm.nih.gov/pubmed/28081167 http://dx.doi.org/10.1371/journal.pone.0169753 |
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