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Mapping Whole-Transcriptome Splicing in Mouse Hematopoietic Stem Cells

Hematopoietic stem cells (HSCs) are rare cells that generate all the various types of blood and immune cells. High-quality transcriptome data have enabled the identification of significant genes for HSCs. However, most genes are expressed in various forms by alternative splicing (AS), extending tran...

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Autores principales: Goldstein, Oron, Meyer, Karin, Greenshpan, Yariv, Bujanover, Nir, Feigin, Mili, Ner-Gaon, Hadas, Shay, Tal, Gazit, Roi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5233452/
https://www.ncbi.nlm.nih.gov/pubmed/28041879
http://dx.doi.org/10.1016/j.stemcr.2016.12.002
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author Goldstein, Oron
Meyer, Karin
Greenshpan, Yariv
Bujanover, Nir
Feigin, Mili
Ner-Gaon, Hadas
Shay, Tal
Gazit, Roi
author_facet Goldstein, Oron
Meyer, Karin
Greenshpan, Yariv
Bujanover, Nir
Feigin, Mili
Ner-Gaon, Hadas
Shay, Tal
Gazit, Roi
author_sort Goldstein, Oron
collection PubMed
description Hematopoietic stem cells (HSCs) are rare cells that generate all the various types of blood and immune cells. High-quality transcriptome data have enabled the identification of significant genes for HSCs. However, most genes are expressed in various forms by alternative splicing (AS), extending transcriptome complexity. Here, we delineate AS to determine which isoforms are expressed in mouse HSCs. Our analysis of microarray and RNA-sequencing data includes differential expression of splicing factors that may regulate AS, and a complete map of splicing isoforms. Multiple types of isoforms for known HSC genes and unannotated splicing that may alter gene function are presented. Transcriptome-wide identification of genes and their respective isoforms in mouse HSCs will open another dimension for adult stem cells.
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spelling pubmed-52334522017-01-23 Mapping Whole-Transcriptome Splicing in Mouse Hematopoietic Stem Cells Goldstein, Oron Meyer, Karin Greenshpan, Yariv Bujanover, Nir Feigin, Mili Ner-Gaon, Hadas Shay, Tal Gazit, Roi Stem Cell Reports Article Hematopoietic stem cells (HSCs) are rare cells that generate all the various types of blood and immune cells. High-quality transcriptome data have enabled the identification of significant genes for HSCs. However, most genes are expressed in various forms by alternative splicing (AS), extending transcriptome complexity. Here, we delineate AS to determine which isoforms are expressed in mouse HSCs. Our analysis of microarray and RNA-sequencing data includes differential expression of splicing factors that may regulate AS, and a complete map of splicing isoforms. Multiple types of isoforms for known HSC genes and unannotated splicing that may alter gene function are presented. Transcriptome-wide identification of genes and their respective isoforms in mouse HSCs will open another dimension for adult stem cells. Elsevier 2016-12-29 /pmc/articles/PMC5233452/ /pubmed/28041879 http://dx.doi.org/10.1016/j.stemcr.2016.12.002 Text en © 2017 The Author(s) http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Goldstein, Oron
Meyer, Karin
Greenshpan, Yariv
Bujanover, Nir
Feigin, Mili
Ner-Gaon, Hadas
Shay, Tal
Gazit, Roi
Mapping Whole-Transcriptome Splicing in Mouse Hematopoietic Stem Cells
title Mapping Whole-Transcriptome Splicing in Mouse Hematopoietic Stem Cells
title_full Mapping Whole-Transcriptome Splicing in Mouse Hematopoietic Stem Cells
title_fullStr Mapping Whole-Transcriptome Splicing in Mouse Hematopoietic Stem Cells
title_full_unstemmed Mapping Whole-Transcriptome Splicing in Mouse Hematopoietic Stem Cells
title_short Mapping Whole-Transcriptome Splicing in Mouse Hematopoietic Stem Cells
title_sort mapping whole-transcriptome splicing in mouse hematopoietic stem cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5233452/
https://www.ncbi.nlm.nih.gov/pubmed/28041879
http://dx.doi.org/10.1016/j.stemcr.2016.12.002
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