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Mapping Whole-Transcriptome Splicing in Mouse Hematopoietic Stem Cells
Hematopoietic stem cells (HSCs) are rare cells that generate all the various types of blood and immune cells. High-quality transcriptome data have enabled the identification of significant genes for HSCs. However, most genes are expressed in various forms by alternative splicing (AS), extending tran...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5233452/ https://www.ncbi.nlm.nih.gov/pubmed/28041879 http://dx.doi.org/10.1016/j.stemcr.2016.12.002 |
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author | Goldstein, Oron Meyer, Karin Greenshpan, Yariv Bujanover, Nir Feigin, Mili Ner-Gaon, Hadas Shay, Tal Gazit, Roi |
author_facet | Goldstein, Oron Meyer, Karin Greenshpan, Yariv Bujanover, Nir Feigin, Mili Ner-Gaon, Hadas Shay, Tal Gazit, Roi |
author_sort | Goldstein, Oron |
collection | PubMed |
description | Hematopoietic stem cells (HSCs) are rare cells that generate all the various types of blood and immune cells. High-quality transcriptome data have enabled the identification of significant genes for HSCs. However, most genes are expressed in various forms by alternative splicing (AS), extending transcriptome complexity. Here, we delineate AS to determine which isoforms are expressed in mouse HSCs. Our analysis of microarray and RNA-sequencing data includes differential expression of splicing factors that may regulate AS, and a complete map of splicing isoforms. Multiple types of isoforms for known HSC genes and unannotated splicing that may alter gene function are presented. Transcriptome-wide identification of genes and their respective isoforms in mouse HSCs will open another dimension for adult stem cells. |
format | Online Article Text |
id | pubmed-5233452 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-52334522017-01-23 Mapping Whole-Transcriptome Splicing in Mouse Hematopoietic Stem Cells Goldstein, Oron Meyer, Karin Greenshpan, Yariv Bujanover, Nir Feigin, Mili Ner-Gaon, Hadas Shay, Tal Gazit, Roi Stem Cell Reports Article Hematopoietic stem cells (HSCs) are rare cells that generate all the various types of blood and immune cells. High-quality transcriptome data have enabled the identification of significant genes for HSCs. However, most genes are expressed in various forms by alternative splicing (AS), extending transcriptome complexity. Here, we delineate AS to determine which isoforms are expressed in mouse HSCs. Our analysis of microarray and RNA-sequencing data includes differential expression of splicing factors that may regulate AS, and a complete map of splicing isoforms. Multiple types of isoforms for known HSC genes and unannotated splicing that may alter gene function are presented. Transcriptome-wide identification of genes and their respective isoforms in mouse HSCs will open another dimension for adult stem cells. Elsevier 2016-12-29 /pmc/articles/PMC5233452/ /pubmed/28041879 http://dx.doi.org/10.1016/j.stemcr.2016.12.002 Text en © 2017 The Author(s) http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Article Goldstein, Oron Meyer, Karin Greenshpan, Yariv Bujanover, Nir Feigin, Mili Ner-Gaon, Hadas Shay, Tal Gazit, Roi Mapping Whole-Transcriptome Splicing in Mouse Hematopoietic Stem Cells |
title | Mapping Whole-Transcriptome Splicing in Mouse Hematopoietic Stem Cells |
title_full | Mapping Whole-Transcriptome Splicing in Mouse Hematopoietic Stem Cells |
title_fullStr | Mapping Whole-Transcriptome Splicing in Mouse Hematopoietic Stem Cells |
title_full_unstemmed | Mapping Whole-Transcriptome Splicing in Mouse Hematopoietic Stem Cells |
title_short | Mapping Whole-Transcriptome Splicing in Mouse Hematopoietic Stem Cells |
title_sort | mapping whole-transcriptome splicing in mouse hematopoietic stem cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5233452/ https://www.ncbi.nlm.nih.gov/pubmed/28041879 http://dx.doi.org/10.1016/j.stemcr.2016.12.002 |
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