Cargando…

LPS promotes Th2 dependent sensitisation leading to anaphylaxis in a Pru p 3 mouse model

Pru p 3 is the major peach allergen in the Mediterranean area. It frequently elicits severe reactions, limiting its study in humans, raising the need for animal models to investigate the immunological mechanisms involved. However, no anaphylaxis model exists for Pru p 3. We aimed to develop a model...

Descripción completa

Detalles Bibliográficos
Autores principales: Rodriguez, Maria J., Aranda, Ana, Fernandez, Tahia D., Cubells-Baeza, Nuria, Torres, Maria J., Gomez, Francisca, Palomares, Francisca, Perkins, James R., Rojo, Javier, Diaz-Perales, Araceli, Mayorga, Cristobalina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5233975/
https://www.ncbi.nlm.nih.gov/pubmed/28084419
http://dx.doi.org/10.1038/srep40449
_version_ 1782494913487699968
author Rodriguez, Maria J.
Aranda, Ana
Fernandez, Tahia D.
Cubells-Baeza, Nuria
Torres, Maria J.
Gomez, Francisca
Palomares, Francisca
Perkins, James R.
Rojo, Javier
Diaz-Perales, Araceli
Mayorga, Cristobalina
author_facet Rodriguez, Maria J.
Aranda, Ana
Fernandez, Tahia D.
Cubells-Baeza, Nuria
Torres, Maria J.
Gomez, Francisca
Palomares, Francisca
Perkins, James R.
Rojo, Javier
Diaz-Perales, Araceli
Mayorga, Cristobalina
author_sort Rodriguez, Maria J.
collection PubMed
description Pru p 3 is the major peach allergen in the Mediterranean area. It frequently elicits severe reactions, limiting its study in humans, raising the need for animal models to investigate the immunological mechanisms involved. However, no anaphylaxis model exists for Pru p 3. We aimed to develop a model of peach anaphylaxis by sensitising mice with Pru p 3 in combination with lipopolysaccharide (LPS) as an adjuvant. Four groups of mice were sensitised intranasally: untreated; treated with Pru p 3; treated with LPS; treated with Pru p 3 + LPS. After sensitisation mice were intraperitoneally challenged with Pru p 3 and in vivo and in vitro parameters were evaluated. Only mice in the Pru p 3 + LPS group showed anaphylaxis symptoms, including a decrease in temperature. Determination of in vitro parameters showed a Th2 response with an increase of Pru p 3-specific IgE and IgG1. Moreover, at the cellular level, we found increased levels of IgE and IgG1 secreting Pru p 3-specific cells and a proliferative CD4(+) T-cell response. These results demonstrate that Pru p 3-specific anaphylaxis can be generated after nasal sensitisation to Pru p 3 in combination with LPS. This is a promising model for evaluating food allergy immunotherapies.
format Online
Article
Text
id pubmed-5233975
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Nature Publishing Group
record_format MEDLINE/PubMed
spelling pubmed-52339752017-01-17 LPS promotes Th2 dependent sensitisation leading to anaphylaxis in a Pru p 3 mouse model Rodriguez, Maria J. Aranda, Ana Fernandez, Tahia D. Cubells-Baeza, Nuria Torres, Maria J. Gomez, Francisca Palomares, Francisca Perkins, James R. Rojo, Javier Diaz-Perales, Araceli Mayorga, Cristobalina Sci Rep Article Pru p 3 is the major peach allergen in the Mediterranean area. It frequently elicits severe reactions, limiting its study in humans, raising the need for animal models to investigate the immunological mechanisms involved. However, no anaphylaxis model exists for Pru p 3. We aimed to develop a model of peach anaphylaxis by sensitising mice with Pru p 3 in combination with lipopolysaccharide (LPS) as an adjuvant. Four groups of mice were sensitised intranasally: untreated; treated with Pru p 3; treated with LPS; treated with Pru p 3 + LPS. After sensitisation mice were intraperitoneally challenged with Pru p 3 and in vivo and in vitro parameters were evaluated. Only mice in the Pru p 3 + LPS group showed anaphylaxis symptoms, including a decrease in temperature. Determination of in vitro parameters showed a Th2 response with an increase of Pru p 3-specific IgE and IgG1. Moreover, at the cellular level, we found increased levels of IgE and IgG1 secreting Pru p 3-specific cells and a proliferative CD4(+) T-cell response. These results demonstrate that Pru p 3-specific anaphylaxis can be generated after nasal sensitisation to Pru p 3 in combination with LPS. This is a promising model for evaluating food allergy immunotherapies. Nature Publishing Group 2017-01-13 /pmc/articles/PMC5233975/ /pubmed/28084419 http://dx.doi.org/10.1038/srep40449 Text en Copyright © 2017, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Rodriguez, Maria J.
Aranda, Ana
Fernandez, Tahia D.
Cubells-Baeza, Nuria
Torres, Maria J.
Gomez, Francisca
Palomares, Francisca
Perkins, James R.
Rojo, Javier
Diaz-Perales, Araceli
Mayorga, Cristobalina
LPS promotes Th2 dependent sensitisation leading to anaphylaxis in a Pru p 3 mouse model
title LPS promotes Th2 dependent sensitisation leading to anaphylaxis in a Pru p 3 mouse model
title_full LPS promotes Th2 dependent sensitisation leading to anaphylaxis in a Pru p 3 mouse model
title_fullStr LPS promotes Th2 dependent sensitisation leading to anaphylaxis in a Pru p 3 mouse model
title_full_unstemmed LPS promotes Th2 dependent sensitisation leading to anaphylaxis in a Pru p 3 mouse model
title_short LPS promotes Th2 dependent sensitisation leading to anaphylaxis in a Pru p 3 mouse model
title_sort lps promotes th2 dependent sensitisation leading to anaphylaxis in a pru p 3 mouse model
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5233975/
https://www.ncbi.nlm.nih.gov/pubmed/28084419
http://dx.doi.org/10.1038/srep40449
work_keys_str_mv AT rodriguezmariaj lpspromotesth2dependentsensitisationleadingtoanaphylaxisinaprup3mousemodel
AT arandaana lpspromotesth2dependentsensitisationleadingtoanaphylaxisinaprup3mousemodel
AT fernandeztahiad lpspromotesth2dependentsensitisationleadingtoanaphylaxisinaprup3mousemodel
AT cubellsbaezanuria lpspromotesth2dependentsensitisationleadingtoanaphylaxisinaprup3mousemodel
AT torresmariaj lpspromotesth2dependentsensitisationleadingtoanaphylaxisinaprup3mousemodel
AT gomezfrancisca lpspromotesth2dependentsensitisationleadingtoanaphylaxisinaprup3mousemodel
AT palomaresfrancisca lpspromotesth2dependentsensitisationleadingtoanaphylaxisinaprup3mousemodel
AT perkinsjamesr lpspromotesth2dependentsensitisationleadingtoanaphylaxisinaprup3mousemodel
AT rojojavier lpspromotesth2dependentsensitisationleadingtoanaphylaxisinaprup3mousemodel
AT diazperalesaraceli lpspromotesth2dependentsensitisationleadingtoanaphylaxisinaprup3mousemodel
AT mayorgacristobalina lpspromotesth2dependentsensitisationleadingtoanaphylaxisinaprup3mousemodel