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Molecular characterization of field infectious bursal disease virus isolates from Nigeria

AIM: To characterize field isolates of infectious bursal disease virus (IBDV) from outbreaks in nine states in Nigeria through reverse transcription polymerase chain reaction (RT-PCR) and sequence analysis of portions of the VP2 and VP1 genes and to determine the presence or absence of reassortant v...

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Autores principales: Nwagbo, Ijeoma O., Shittu, Ismaila, Nwosuh, Chika I., Ezeifeka, George O., Odibo, Frederick J. C., Michel, Linda O., Jackwood, Daral J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Veterinary World 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5234057/
https://www.ncbi.nlm.nih.gov/pubmed/28096615
http://dx.doi.org/10.14202/vetworld.2016.1420-1428
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author Nwagbo, Ijeoma O.
Shittu, Ismaila
Nwosuh, Chika I.
Ezeifeka, George O.
Odibo, Frederick J. C.
Michel, Linda O.
Jackwood, Daral J.
author_facet Nwagbo, Ijeoma O.
Shittu, Ismaila
Nwosuh, Chika I.
Ezeifeka, George O.
Odibo, Frederick J. C.
Michel, Linda O.
Jackwood, Daral J.
author_sort Nwagbo, Ijeoma O.
collection PubMed
description AIM: To characterize field isolates of infectious bursal disease virus (IBDV) from outbreaks in nine states in Nigeria through reverse transcription polymerase chain reaction (RT-PCR) and sequence analysis of portions of the VP2 and VP1 genes and to determine the presence or absence of reassortant viruses. MATERIALS AND METHODS: A total of 377 bursa samples were collected from 201 suspected IBD outbreaks during 2009 to 2014 from nine states in Nigeria. Samples were subjected to RT-PCR using VP2 and VP1 gene specific primers, and the resulting PCR products were sequenced. RESULTS: A total of 143 samples were positive for IBDV by RT-PCR. These assays amplified a 743 bp fragment from nt 701 to 1444 in the IBDV VP2 hypervariable region (hvVP2) of segment A and a 722 bp fragment from nt 168 to 889 in the VP1 gene of segment B. RT-PCR products were sequenced, aligned and compared with reference IBDV sequences obtained from GenBank. All but one hvVP2 sequence showed similarity to very virulent IBDV (vvIBDV) reference strains, yet only 3 of the VP1 67 VP1 sequences showed similarity to the VP1 gene of vvIBDV. Phylogenetic analysis revealed a new lineage of Nigerian reassortant IBDV strains. CONCLUSION: Phylogenetic analysis of partial sequences of genome segment A and B of IBDV in Nigeria confirmed the existence of vvIBDV in Nigeria. In addition, we noted the existence of reassortant IBDV strains with novel triplet amino acid motifs at positions 145, 146 and 147 in the reassorted Nigerian IBDV.
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spelling pubmed-52340572017-01-17 Molecular characterization of field infectious bursal disease virus isolates from Nigeria Nwagbo, Ijeoma O. Shittu, Ismaila Nwosuh, Chika I. Ezeifeka, George O. Odibo, Frederick J. C. Michel, Linda O. Jackwood, Daral J. Vet World Research Article AIM: To characterize field isolates of infectious bursal disease virus (IBDV) from outbreaks in nine states in Nigeria through reverse transcription polymerase chain reaction (RT-PCR) and sequence analysis of portions of the VP2 and VP1 genes and to determine the presence or absence of reassortant viruses. MATERIALS AND METHODS: A total of 377 bursa samples were collected from 201 suspected IBD outbreaks during 2009 to 2014 from nine states in Nigeria. Samples were subjected to RT-PCR using VP2 and VP1 gene specific primers, and the resulting PCR products were sequenced. RESULTS: A total of 143 samples were positive for IBDV by RT-PCR. These assays amplified a 743 bp fragment from nt 701 to 1444 in the IBDV VP2 hypervariable region (hvVP2) of segment A and a 722 bp fragment from nt 168 to 889 in the VP1 gene of segment B. RT-PCR products were sequenced, aligned and compared with reference IBDV sequences obtained from GenBank. All but one hvVP2 sequence showed similarity to very virulent IBDV (vvIBDV) reference strains, yet only 3 of the VP1 67 VP1 sequences showed similarity to the VP1 gene of vvIBDV. Phylogenetic analysis revealed a new lineage of Nigerian reassortant IBDV strains. CONCLUSION: Phylogenetic analysis of partial sequences of genome segment A and B of IBDV in Nigeria confirmed the existence of vvIBDV in Nigeria. In addition, we noted the existence of reassortant IBDV strains with novel triplet amino acid motifs at positions 145, 146 and 147 in the reassorted Nigerian IBDV. Veterinary World 2016-12 2016-12-15 /pmc/articles/PMC5234057/ /pubmed/28096615 http://dx.doi.org/10.14202/vetworld.2016.1420-1428 Text en Copyright: © Nwagbo, et al. http://creativecommons.org/licenses/by/4.0 Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Nwagbo, Ijeoma O.
Shittu, Ismaila
Nwosuh, Chika I.
Ezeifeka, George O.
Odibo, Frederick J. C.
Michel, Linda O.
Jackwood, Daral J.
Molecular characterization of field infectious bursal disease virus isolates from Nigeria
title Molecular characterization of field infectious bursal disease virus isolates from Nigeria
title_full Molecular characterization of field infectious bursal disease virus isolates from Nigeria
title_fullStr Molecular characterization of field infectious bursal disease virus isolates from Nigeria
title_full_unstemmed Molecular characterization of field infectious bursal disease virus isolates from Nigeria
title_short Molecular characterization of field infectious bursal disease virus isolates from Nigeria
title_sort molecular characterization of field infectious bursal disease virus isolates from nigeria
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5234057/
https://www.ncbi.nlm.nih.gov/pubmed/28096615
http://dx.doi.org/10.14202/vetworld.2016.1420-1428
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