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Phenotypic and genotypic characterization of locally isolated Salmonella strains used in preparation of Salmonella antigens in Egypt
AIM: This work was conducted to study the phenotypic and genotypic characterization of locally isolated Salmonella strains (Salmonella Pullorum, Salmonella Enteritidis, and Salmonella Typhimurium) from poultry used in the preparation of Salmonella antigens in Egypt. MATERIALS AND METHODS: The phenot...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Veterinary World
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5234059/ https://www.ncbi.nlm.nih.gov/pubmed/28096617 http://dx.doi.org/10.14202/vetworld.2016.1435-1439 |
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author | Ibrahim, Hazem Mohammed El-Moaty, Dalia Ahmed Mohammed Abd Ahmed, Hanan Ali El-Enbaawy, Mona Ibrahim |
author_facet | Ibrahim, Hazem Mohammed El-Moaty, Dalia Ahmed Mohammed Abd Ahmed, Hanan Ali El-Enbaawy, Mona Ibrahim |
author_sort | Ibrahim, Hazem Mohammed |
collection | PubMed |
description | AIM: This work was conducted to study the phenotypic and genotypic characterization of locally isolated Salmonella strains (Salmonella Pullorum, Salmonella Enteritidis, and Salmonella Typhimurium) from poultry used in the preparation of Salmonella antigens in Egypt. MATERIALS AND METHODS: The phenotypic characterization of Salmonella strains was done using standard microbiological, biochemical, and serological techniques. Molecular identification was done using different sets of primers on different genes using different polymerase chain reaction (PCR) techniques. RESULTS: The phenotypic characterization of Salmonella strains was confirmed. Molecular identification revealed detection of 284 bp fragment of InvA gene in all studied Salmonella strains. Furthermore, multiplex PCR was used for more confirmation of being Salmonella spp., generally at 429 bp as well as genotyping of Salmonella Typhimurium and Salmonella Enteritidis at 559 and 312 bp, respectively, in one reaction. CONCLUSION: The locally isolated field Salmonella strains were confirmed phenotypically and genotypically to be Salmonella Enteritidis, and Salmonella Typhimurium and could be used for the preparation of Salmonella antigens. |
format | Online Article Text |
id | pubmed-5234059 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Veterinary World |
record_format | MEDLINE/PubMed |
spelling | pubmed-52340592017-01-17 Phenotypic and genotypic characterization of locally isolated Salmonella strains used in preparation of Salmonella antigens in Egypt Ibrahim, Hazem Mohammed El-Moaty, Dalia Ahmed Mohammed Abd Ahmed, Hanan Ali El-Enbaawy, Mona Ibrahim Vet World Research Article AIM: This work was conducted to study the phenotypic and genotypic characterization of locally isolated Salmonella strains (Salmonella Pullorum, Salmonella Enteritidis, and Salmonella Typhimurium) from poultry used in the preparation of Salmonella antigens in Egypt. MATERIALS AND METHODS: The phenotypic characterization of Salmonella strains was done using standard microbiological, biochemical, and serological techniques. Molecular identification was done using different sets of primers on different genes using different polymerase chain reaction (PCR) techniques. RESULTS: The phenotypic characterization of Salmonella strains was confirmed. Molecular identification revealed detection of 284 bp fragment of InvA gene in all studied Salmonella strains. Furthermore, multiplex PCR was used for more confirmation of being Salmonella spp., generally at 429 bp as well as genotyping of Salmonella Typhimurium and Salmonella Enteritidis at 559 and 312 bp, respectively, in one reaction. CONCLUSION: The locally isolated field Salmonella strains were confirmed phenotypically and genotypically to be Salmonella Enteritidis, and Salmonella Typhimurium and could be used for the preparation of Salmonella antigens. Veterinary World 2016-12 2016-12-17 /pmc/articles/PMC5234059/ /pubmed/28096617 http://dx.doi.org/10.14202/vetworld.2016.1435-1439 Text en Copyright: © Ibrahim, et al. http://creativecommons.org/licenses/by/4.0 Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Ibrahim, Hazem Mohammed El-Moaty, Dalia Ahmed Mohammed Abd Ahmed, Hanan Ali El-Enbaawy, Mona Ibrahim Phenotypic and genotypic characterization of locally isolated Salmonella strains used in preparation of Salmonella antigens in Egypt |
title | Phenotypic and genotypic characterization of locally isolated Salmonella strains used in preparation of Salmonella antigens in Egypt |
title_full | Phenotypic and genotypic characterization of locally isolated Salmonella strains used in preparation of Salmonella antigens in Egypt |
title_fullStr | Phenotypic and genotypic characterization of locally isolated Salmonella strains used in preparation of Salmonella antigens in Egypt |
title_full_unstemmed | Phenotypic and genotypic characterization of locally isolated Salmonella strains used in preparation of Salmonella antigens in Egypt |
title_short | Phenotypic and genotypic characterization of locally isolated Salmonella strains used in preparation of Salmonella antigens in Egypt |
title_sort | phenotypic and genotypic characterization of locally isolated salmonella strains used in preparation of salmonella antigens in egypt |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5234059/ https://www.ncbi.nlm.nih.gov/pubmed/28096617 http://dx.doi.org/10.14202/vetworld.2016.1435-1439 |
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