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Synthetic and natural antioxidants attenuate cisplatin-induced vomiting

BACKGROUND: Synthetic and natural antioxidants including Bacopa monnieri (L.) Pennell (Scrophulariaceae) which also possess anti-dopaminergic properties, have been proposed to be useful for emetogenic chemotherapy. In this study, synthetic [N-(2-mercaptopropionyl) glycine (MPG), vitamin C (Vit-C)] a...

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Autores principales: Alam, Javaid, Subhan, Fazal, Ullah, Ihsan, Shahid, Muhammad, Ali, Gowhar, Sewell, Robert D. E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5234122/
https://www.ncbi.nlm.nih.gov/pubmed/28081725
http://dx.doi.org/10.1186/s40360-016-0110-9
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author Alam, Javaid
Subhan, Fazal
Ullah, Ihsan
Shahid, Muhammad
Ali, Gowhar
Sewell, Robert D. E.
author_facet Alam, Javaid
Subhan, Fazal
Ullah, Ihsan
Shahid, Muhammad
Ali, Gowhar
Sewell, Robert D. E.
author_sort Alam, Javaid
collection PubMed
description BACKGROUND: Synthetic and natural antioxidants including Bacopa monnieri (L.) Pennell (Scrophulariaceae) which also possess anti-dopaminergic properties, have been proposed to be useful for emetogenic chemotherapy. In this study, synthetic [N-(2-mercaptopropionyl) glycine (MPG), vitamin C (Vit-C)] and natural [grape seed proanthocyanidin (GP), B. monnieri n-butanolic fraction (BM-ButFr)] antioxidants and their combinations were evaluated against cisplatin-induced emesis in pigeons during a 24 h observation period. METHODS: Emesis was induced using cisplatin (7.0 mg/kg, i.v). MPG (10, 20, 30 mg/kg), Vit-C (100, 200, 300 mg/kg), GP (50, 100, 150 mg/kg) and BM-ButFr (5, 10, 20 mg/kg) and their combinations were administered i.m., 15 min before cisplatin administration. The number of vomiting bouts, retching, emetic latency and % weight loss were recorded to assess antiemetic potential. Antioxidant activity was evaluated by the DPPH free radical scavenging assay (FRSA). RESULTS: Significant attenuation of vomiting bouts, retching, % weight loss along with an increase in latency was produced by all the antioxidants and their combinations compared to cisplatin alone and this is the first report of this activity of GP in pigeons. Low EC(50) values in the FRSA for MPG (67.66 μg/mL), Vit-C (69.42 μg/mL), GP (6.498 μg/mL) and BM-ButFr (55.61 μg/mL) compared to BHT standard (98.17 μg/mL) demonstrated their radical scavenging capacity. Correlation between the antioxidant activity and antiemetic efficacy disclosed a high degree of correlation for the tested antioxidants. CONCLUSION: The selected synthetic and natural antioxidants and their combinations were able to attenuate cisplatin-induced vomiting, which correlated with their potent in vitro antioxidant activity. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s40360-016-0110-9) contains supplementary material, which is available to authorized users.
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spelling pubmed-52341222017-01-17 Synthetic and natural antioxidants attenuate cisplatin-induced vomiting Alam, Javaid Subhan, Fazal Ullah, Ihsan Shahid, Muhammad Ali, Gowhar Sewell, Robert D. E. BMC Pharmacol Toxicol Research Article BACKGROUND: Synthetic and natural antioxidants including Bacopa monnieri (L.) Pennell (Scrophulariaceae) which also possess anti-dopaminergic properties, have been proposed to be useful for emetogenic chemotherapy. In this study, synthetic [N-(2-mercaptopropionyl) glycine (MPG), vitamin C (Vit-C)] and natural [grape seed proanthocyanidin (GP), B. monnieri n-butanolic fraction (BM-ButFr)] antioxidants and their combinations were evaluated against cisplatin-induced emesis in pigeons during a 24 h observation period. METHODS: Emesis was induced using cisplatin (7.0 mg/kg, i.v). MPG (10, 20, 30 mg/kg), Vit-C (100, 200, 300 mg/kg), GP (50, 100, 150 mg/kg) and BM-ButFr (5, 10, 20 mg/kg) and their combinations were administered i.m., 15 min before cisplatin administration. The number of vomiting bouts, retching, emetic latency and % weight loss were recorded to assess antiemetic potential. Antioxidant activity was evaluated by the DPPH free radical scavenging assay (FRSA). RESULTS: Significant attenuation of vomiting bouts, retching, % weight loss along with an increase in latency was produced by all the antioxidants and their combinations compared to cisplatin alone and this is the first report of this activity of GP in pigeons. Low EC(50) values in the FRSA for MPG (67.66 μg/mL), Vit-C (69.42 μg/mL), GP (6.498 μg/mL) and BM-ButFr (55.61 μg/mL) compared to BHT standard (98.17 μg/mL) demonstrated their radical scavenging capacity. Correlation between the antioxidant activity and antiemetic efficacy disclosed a high degree of correlation for the tested antioxidants. CONCLUSION: The selected synthetic and natural antioxidants and their combinations were able to attenuate cisplatin-induced vomiting, which correlated with their potent in vitro antioxidant activity. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s40360-016-0110-9) contains supplementary material, which is available to authorized users. BioMed Central 2017-01-13 /pmc/articles/PMC5234122/ /pubmed/28081725 http://dx.doi.org/10.1186/s40360-016-0110-9 Text en © The Author(s). 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Alam, Javaid
Subhan, Fazal
Ullah, Ihsan
Shahid, Muhammad
Ali, Gowhar
Sewell, Robert D. E.
Synthetic and natural antioxidants attenuate cisplatin-induced vomiting
title Synthetic and natural antioxidants attenuate cisplatin-induced vomiting
title_full Synthetic and natural antioxidants attenuate cisplatin-induced vomiting
title_fullStr Synthetic and natural antioxidants attenuate cisplatin-induced vomiting
title_full_unstemmed Synthetic and natural antioxidants attenuate cisplatin-induced vomiting
title_short Synthetic and natural antioxidants attenuate cisplatin-induced vomiting
title_sort synthetic and natural antioxidants attenuate cisplatin-induced vomiting
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5234122/
https://www.ncbi.nlm.nih.gov/pubmed/28081725
http://dx.doi.org/10.1186/s40360-016-0110-9
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