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Effects of Inhalable Microparticles of Seonpyejeongcheon-Tang in an Asthma Mouse Model: - Effects of Microparticles of SJT -
OBJECTIVES: Allergic asthma generally presents with symptoms of wheezing, coughing, breathlessness, and airway inflammation. Seonpyejeongcheon-tang (SJT) consists of 12 herbs. It originated from Jeongcheon-tang (JT), also known as Ding-chuan-tang, composed of 7 herbs, in She-sheng-zhong-miao-fang. T...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
KOREAN PHARMACOPUNCTURE INSTITUTE
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5234350/ https://www.ncbi.nlm.nih.gov/pubmed/28097040 http://dx.doi.org/10.3831/KPI.2016.19.031 |
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author | Yang, Won-Kyung Lee, Chul-Hwa Kim, Min-Hee Kim, Seung-Hyeong Choi, Hae-Yoon Yeo, Yoon Park, Yang-Chun |
author_facet | Yang, Won-Kyung Lee, Chul-Hwa Kim, Min-Hee Kim, Seung-Hyeong Choi, Hae-Yoon Yeo, Yoon Park, Yang-Chun |
author_sort | Yang, Won-Kyung |
collection | PubMed |
description | OBJECTIVES: Allergic asthma generally presents with symptoms of wheezing, coughing, breathlessness, and airway inflammation. Seonpyejeongcheon-tang (SJT) consists of 12 herbs. It originated from Jeongcheon-tang (JT), also known as Ding-chuan-tang, composed of 7 herbs, in She-sheng-zhong-miao-fang. This study aimed to evaluate the effects of local delivery of SJT via inhalable microparticles in an asthma mouse model. METHODS: Microparticles containing SJT were produced by spray-drying with leucine as an excipient. SJT microparticles were evaluated with respect to their aerodynamic properties, in vitro cytotoxicity, in vivo toxicity, and therapeutic effects on ovalbumin (OVA)-induced asthma in comparison with orally-administered SJT. RESULTS: SJT microparticles provided desirable aerodynamic properties (fine particle fraction of 48.9% ± 6.4% and mass median aerodynamic diameter of 3.7 ± 0.3 μm). SJT microparticles did not show any cytotoxicity against RAW 264.7 macrophages at concentrations of 0.01 - 3 mg/mL. Inhaled SJT microparticles decreased the levels of IL-4, IL-5, IL-13, IL-17A, eotaxin and OVA-IgE in bronchoalveolar lavage fluid (BALF) in mice with OVA-induced asthma. These effects were verified by histological evaluation of the levels of infiltration of inflammatory cells and collagen, destructions of alveoli and bronchioles, and hyperplasia of goblet cells in lung tissues. The effects of SJT microparticles in the asthma model were equivalent to those of orally-administered SJT extract. CONCLUSION: This study suggests that SJT is a promising agent for inhalation therapy for patients with asthma. |
format | Online Article Text |
id | pubmed-5234350 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | KOREAN PHARMACOPUNCTURE INSTITUTE |
record_format | MEDLINE/PubMed |
spelling | pubmed-52343502017-01-17 Effects of Inhalable Microparticles of Seonpyejeongcheon-Tang in an Asthma Mouse Model: - Effects of Microparticles of SJT - Yang, Won-Kyung Lee, Chul-Hwa Kim, Min-Hee Kim, Seung-Hyeong Choi, Hae-Yoon Yeo, Yoon Park, Yang-Chun J Pharmacopuncture Original Article OBJECTIVES: Allergic asthma generally presents with symptoms of wheezing, coughing, breathlessness, and airway inflammation. Seonpyejeongcheon-tang (SJT) consists of 12 herbs. It originated from Jeongcheon-tang (JT), also known as Ding-chuan-tang, composed of 7 herbs, in She-sheng-zhong-miao-fang. This study aimed to evaluate the effects of local delivery of SJT via inhalable microparticles in an asthma mouse model. METHODS: Microparticles containing SJT were produced by spray-drying with leucine as an excipient. SJT microparticles were evaluated with respect to their aerodynamic properties, in vitro cytotoxicity, in vivo toxicity, and therapeutic effects on ovalbumin (OVA)-induced asthma in comparison with orally-administered SJT. RESULTS: SJT microparticles provided desirable aerodynamic properties (fine particle fraction of 48.9% ± 6.4% and mass median aerodynamic diameter of 3.7 ± 0.3 μm). SJT microparticles did not show any cytotoxicity against RAW 264.7 macrophages at concentrations of 0.01 - 3 mg/mL. Inhaled SJT microparticles decreased the levels of IL-4, IL-5, IL-13, IL-17A, eotaxin and OVA-IgE in bronchoalveolar lavage fluid (BALF) in mice with OVA-induced asthma. These effects were verified by histological evaluation of the levels of infiltration of inflammatory cells and collagen, destructions of alveoli and bronchioles, and hyperplasia of goblet cells in lung tissues. The effects of SJT microparticles in the asthma model were equivalent to those of orally-administered SJT extract. CONCLUSION: This study suggests that SJT is a promising agent for inhalation therapy for patients with asthma. KOREAN PHARMACOPUNCTURE INSTITUTE 2016-12 /pmc/articles/PMC5234350/ /pubmed/28097040 http://dx.doi.org/10.3831/KPI.2016.19.031 Text en Copyright ©2016, KOREAN PHARMACOPUNCTURE INSTITUTE http://creativecommons.org/licenses/by-nc/3.0/ This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Yang, Won-Kyung Lee, Chul-Hwa Kim, Min-Hee Kim, Seung-Hyeong Choi, Hae-Yoon Yeo, Yoon Park, Yang-Chun Effects of Inhalable Microparticles of Seonpyejeongcheon-Tang in an Asthma Mouse Model: - Effects of Microparticles of SJT - |
title | Effects of Inhalable Microparticles of Seonpyejeongcheon-Tang in an Asthma Mouse Model: - Effects of Microparticles of SJT - |
title_full | Effects of Inhalable Microparticles of Seonpyejeongcheon-Tang in an Asthma Mouse Model: - Effects of Microparticles of SJT - |
title_fullStr | Effects of Inhalable Microparticles of Seonpyejeongcheon-Tang in an Asthma Mouse Model: - Effects of Microparticles of SJT - |
title_full_unstemmed | Effects of Inhalable Microparticles of Seonpyejeongcheon-Tang in an Asthma Mouse Model: - Effects of Microparticles of SJT - |
title_short | Effects of Inhalable Microparticles of Seonpyejeongcheon-Tang in an Asthma Mouse Model: - Effects of Microparticles of SJT - |
title_sort | effects of inhalable microparticles of seonpyejeongcheon-tang in an asthma mouse model: - effects of microparticles of sjt - |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5234350/ https://www.ncbi.nlm.nih.gov/pubmed/28097040 http://dx.doi.org/10.3831/KPI.2016.19.031 |
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