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The Relaxant Activity of Safranal in Isolated Rat Aortas is Mediated Predominantly via an Endothelium-Independent Mechanism: -Vasodilatory mechanism of safranal-

OBJECTIVES: Safranal is a pharmacologically active component of saffron and is responsible for the unique aroma of saffron. The hypotensive effect of safranal has been shown in previous studies. This study evaluates the mechanism for the vasodilatory effects induced by safranal on isolated rat aorta...

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Autores principales: Razavi, Bibi Marjan, Amanloo, Mojtaba Alipoor, Imenshahidi, Mohsen, Hosseinzadeh, Hossein
Formato: Online Artículo Texto
Lenguaje:English
Publicado: KOREAN PHARMACOPUNCTURE INSTITUTE 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5234352/
https://www.ncbi.nlm.nih.gov/pubmed/28097042
http://dx.doi.org/10.3831/KPI.2016.19.034
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author Razavi, Bibi Marjan
Amanloo, Mojtaba Alipoor
Imenshahidi, Mohsen
Hosseinzadeh, Hossein
author_facet Razavi, Bibi Marjan
Amanloo, Mojtaba Alipoor
Imenshahidi, Mohsen
Hosseinzadeh, Hossein
author_sort Razavi, Bibi Marjan
collection PubMed
description OBJECTIVES: Safranal is a pharmacologically active component of saffron and is responsible for the unique aroma of saffron. The hypotensive effect of safranal has been shown in previous studies. This study evaluates the mechanism for the vasodilatory effects induced by safranal on isolated rat aortas. METHODS: To study the vasodilatory effects of safranal (0.2, 0.4 and 0.8 mM), we contracted isolated rat thoracic aorta rings by using 10(-6)-M phenylephrine (PE) or 80-mM KCl. Dimethyl sulfoxide (DMSO) was used as a control. The vasodilatory effect of safranal was also evaluated both on intact and denuded endothelium aortic rings. Furthermore, to study the role of nitric oxide and prostacyclin in the relaxation induced by safranal, we incubated the aortic rings by using L-NAME (10(-6) M) or indomethacin (10(-5) M), each for 20 minutes. RESULTS: Safranal induced relaxation in endothelium-intact aortic rings precontracted by using PE or KCl in a concentration-dependent manner, with a maximum relaxation of more than 100%. The relaxant activity of safranal was not eliminated by incubating the aortic rings with L-NAME (EC(50) = 0.29 vs. EC(50) = 0.43) or with indomethacin (EC(50) = 0.29 vs. EC(50) = 0.35), where EC(50) is the half maximal effective concentration. Also, the vasodilatory activity of safranal was not modified by endothelial removal. CONCLUSION: This study indicated that relaxant activity of safranal is mediated predominantly through an endothelium- independent mechanism.
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spelling pubmed-52343522017-01-17 The Relaxant Activity of Safranal in Isolated Rat Aortas is Mediated Predominantly via an Endothelium-Independent Mechanism: -Vasodilatory mechanism of safranal- Razavi, Bibi Marjan Amanloo, Mojtaba Alipoor Imenshahidi, Mohsen Hosseinzadeh, Hossein J Pharmacopuncture Original Article OBJECTIVES: Safranal is a pharmacologically active component of saffron and is responsible for the unique aroma of saffron. The hypotensive effect of safranal has been shown in previous studies. This study evaluates the mechanism for the vasodilatory effects induced by safranal on isolated rat aortas. METHODS: To study the vasodilatory effects of safranal (0.2, 0.4 and 0.8 mM), we contracted isolated rat thoracic aorta rings by using 10(-6)-M phenylephrine (PE) or 80-mM KCl. Dimethyl sulfoxide (DMSO) was used as a control. The vasodilatory effect of safranal was also evaluated both on intact and denuded endothelium aortic rings. Furthermore, to study the role of nitric oxide and prostacyclin in the relaxation induced by safranal, we incubated the aortic rings by using L-NAME (10(-6) M) or indomethacin (10(-5) M), each for 20 minutes. RESULTS: Safranal induced relaxation in endothelium-intact aortic rings precontracted by using PE or KCl in a concentration-dependent manner, with a maximum relaxation of more than 100%. The relaxant activity of safranal was not eliminated by incubating the aortic rings with L-NAME (EC(50) = 0.29 vs. EC(50) = 0.43) or with indomethacin (EC(50) = 0.29 vs. EC(50) = 0.35), where EC(50) is the half maximal effective concentration. Also, the vasodilatory activity of safranal was not modified by endothelial removal. CONCLUSION: This study indicated that relaxant activity of safranal is mediated predominantly through an endothelium- independent mechanism. KOREAN PHARMACOPUNCTURE INSTITUTE 2016-12 /pmc/articles/PMC5234352/ /pubmed/28097042 http://dx.doi.org/10.3831/KPI.2016.19.034 Text en Copyright ©2016, KOREAN PHARMACOPUNCTURE INSTITUTE http://creativecommons.org/licenses/by-nc/3.0/ This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Razavi, Bibi Marjan
Amanloo, Mojtaba Alipoor
Imenshahidi, Mohsen
Hosseinzadeh, Hossein
The Relaxant Activity of Safranal in Isolated Rat Aortas is Mediated Predominantly via an Endothelium-Independent Mechanism: -Vasodilatory mechanism of safranal-
title The Relaxant Activity of Safranal in Isolated Rat Aortas is Mediated Predominantly via an Endothelium-Independent Mechanism: -Vasodilatory mechanism of safranal-
title_full The Relaxant Activity of Safranal in Isolated Rat Aortas is Mediated Predominantly via an Endothelium-Independent Mechanism: -Vasodilatory mechanism of safranal-
title_fullStr The Relaxant Activity of Safranal in Isolated Rat Aortas is Mediated Predominantly via an Endothelium-Independent Mechanism: -Vasodilatory mechanism of safranal-
title_full_unstemmed The Relaxant Activity of Safranal in Isolated Rat Aortas is Mediated Predominantly via an Endothelium-Independent Mechanism: -Vasodilatory mechanism of safranal-
title_short The Relaxant Activity of Safranal in Isolated Rat Aortas is Mediated Predominantly via an Endothelium-Independent Mechanism: -Vasodilatory mechanism of safranal-
title_sort relaxant activity of safranal in isolated rat aortas is mediated predominantly via an endothelium-independent mechanism: -vasodilatory mechanism of safranal-
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5234352/
https://www.ncbi.nlm.nih.gov/pubmed/28097042
http://dx.doi.org/10.3831/KPI.2016.19.034
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