Technical advances in flow cytometry-based diagnosis and monitoring of paroxysmal nocturnal hemoglobinuria

OBJECTIVE: To discuss the implementation of technical advances in laboratory diagnosis and monitoring of paroxysmal nocturnal hemoglobinuria for validation of high-sensitivity flow cytometry protocols. METHODS: A retrospective study based on analysis of laboratory data from 745 patient samples submi...

Descripción completa

Detalles Bibliográficos
Autores principales: Correia, Rodolfo Patussi, Bento, Laiz Cameirão, Bortolucci, Ana Carolina Apelle, Alexandre, Anderson Marega, Vaz, Andressa da Costa, Schimidell, Daniela, Pedro, Eduardo de Carvalho, Perin, Fabricio Simões, Nozawa, Sonia Tsukasa, Mendes, Cláudio Ernesto Albers, Barroso, Rodrigo de Souza, Bacal, Nydia Strachman
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Instituto Israelita de Ensino e Pesquisa Albert Einstein 2016
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5234748/
https://www.ncbi.nlm.nih.gov/pubmed/27759825
http://dx.doi.org/10.1590/S1679-45082016AO3641
Descripción
Sumario:OBJECTIVE: To discuss the implementation of technical advances in laboratory diagnosis and monitoring of paroxysmal nocturnal hemoglobinuria for validation of high-sensitivity flow cytometry protocols. METHODS: A retrospective study based on analysis of laboratory data from 745 patient samples submitted to flow cytometry for diagnosis and/or monitoring of paroxysmal nocturnal hemoglobinuria. RESULTS: Implementation of technical advances reduced test costs and improved flow cytometry resolution for paroxysmal nocturnal hemoglobinuria clone detection. CONCLUSION: High-sensitivity flow cytometry allowed more sensitive determination of paroxysmal nocturnal hemoglobinuria clone type and size, particularly in samples with small clones.

Ejemplares similares