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Technical advances in flow cytometry-based diagnosis and monitoring of paroxysmal nocturnal hemoglobinuria
OBJECTIVE: To discuss the implementation of technical advances in laboratory diagnosis and monitoring of paroxysmal nocturnal hemoglobinuria for validation of high-sensitivity flow cytometry protocols. METHODS: A retrospective study based on analysis of laboratory data from 745 patient samples submi...
| Autores principales: | , , , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Instituto Israelita de Ensino e Pesquisa Albert Einstein
2016
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5234748/ https://www.ncbi.nlm.nih.gov/pubmed/27759825 http://dx.doi.org/10.1590/S1679-45082016AO3641 |
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| author | Correia, Rodolfo Patussi Bento, Laiz Cameirão Bortolucci, Ana Carolina Apelle Alexandre, Anderson Marega Vaz, Andressa da Costa Schimidell, Daniela Pedro, Eduardo de Carvalho Perin, Fabricio Simões Nozawa, Sonia Tsukasa Mendes, Cláudio Ernesto Albers Barroso, Rodrigo de Souza Bacal, Nydia Strachman |
| author_facet | Correia, Rodolfo Patussi Bento, Laiz Cameirão Bortolucci, Ana Carolina Apelle Alexandre, Anderson Marega Vaz, Andressa da Costa Schimidell, Daniela Pedro, Eduardo de Carvalho Perin, Fabricio Simões Nozawa, Sonia Tsukasa Mendes, Cláudio Ernesto Albers Barroso, Rodrigo de Souza Bacal, Nydia Strachman |
| author_sort | Correia, Rodolfo Patussi |
| collection | PubMed |
| description | OBJECTIVE: To discuss the implementation of technical advances in laboratory diagnosis and monitoring of paroxysmal nocturnal hemoglobinuria for validation of high-sensitivity flow cytometry protocols. METHODS: A retrospective study based on analysis of laboratory data from 745 patient samples submitted to flow cytometry for diagnosis and/or monitoring of paroxysmal nocturnal hemoglobinuria. RESULTS: Implementation of technical advances reduced test costs and improved flow cytometry resolution for paroxysmal nocturnal hemoglobinuria clone detection. CONCLUSION: High-sensitivity flow cytometry allowed more sensitive determination of paroxysmal nocturnal hemoglobinuria clone type and size, particularly in samples with small clones. |
| format | Online Article Text |
| id | pubmed-5234748 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2016 |
| publisher | Instituto Israelita de Ensino e Pesquisa Albert Einstein |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-52347482017-02-03 Technical advances in flow cytometry-based diagnosis and monitoring of paroxysmal nocturnal hemoglobinuria Correia, Rodolfo Patussi Bento, Laiz Cameirão Bortolucci, Ana Carolina Apelle Alexandre, Anderson Marega Vaz, Andressa da Costa Schimidell, Daniela Pedro, Eduardo de Carvalho Perin, Fabricio Simões Nozawa, Sonia Tsukasa Mendes, Cláudio Ernesto Albers Barroso, Rodrigo de Souza Bacal, Nydia Strachman Einstein (Sao Paulo) Original Article OBJECTIVE: To discuss the implementation of technical advances in laboratory diagnosis and monitoring of paroxysmal nocturnal hemoglobinuria for validation of high-sensitivity flow cytometry protocols. METHODS: A retrospective study based on analysis of laboratory data from 745 patient samples submitted to flow cytometry for diagnosis and/or monitoring of paroxysmal nocturnal hemoglobinuria. RESULTS: Implementation of technical advances reduced test costs and improved flow cytometry resolution for paroxysmal nocturnal hemoglobinuria clone detection. CONCLUSION: High-sensitivity flow cytometry allowed more sensitive determination of paroxysmal nocturnal hemoglobinuria clone type and size, particularly in samples with small clones. Instituto Israelita de Ensino e Pesquisa Albert Einstein 2016 /pmc/articles/PMC5234748/ /pubmed/27759825 http://dx.doi.org/10.1590/S1679-45082016AO3641 Text en http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
| spellingShingle | Original Article Correia, Rodolfo Patussi Bento, Laiz Cameirão Bortolucci, Ana Carolina Apelle Alexandre, Anderson Marega Vaz, Andressa da Costa Schimidell, Daniela Pedro, Eduardo de Carvalho Perin, Fabricio Simões Nozawa, Sonia Tsukasa Mendes, Cláudio Ernesto Albers Barroso, Rodrigo de Souza Bacal, Nydia Strachman Technical advances in flow cytometry-based diagnosis and monitoring of paroxysmal nocturnal hemoglobinuria |
| title | Technical advances in flow cytometry-based diagnosis and monitoring of paroxysmal nocturnal hemoglobinuria |
| title_full | Technical advances in flow cytometry-based diagnosis and monitoring of paroxysmal nocturnal hemoglobinuria |
| title_fullStr | Technical advances in flow cytometry-based diagnosis and monitoring of paroxysmal nocturnal hemoglobinuria |
| title_full_unstemmed | Technical advances in flow cytometry-based diagnosis and monitoring of paroxysmal nocturnal hemoglobinuria |
| title_short | Technical advances in flow cytometry-based diagnosis and monitoring of paroxysmal nocturnal hemoglobinuria |
| title_sort | technical advances in flow cytometry-based diagnosis and monitoring of paroxysmal nocturnal hemoglobinuria |
| topic | Original Article |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5234748/ https://www.ncbi.nlm.nih.gov/pubmed/27759825 http://dx.doi.org/10.1590/S1679-45082016AO3641 |
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