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Ureaplasma Infection Mediated Release of Matrix Metalloproteinase-9 and PGP – a Novel Mechanism of Preterm Rupture of Membranes and Chorioamnionitis

BACKGROUND: Premature rupture of membranes and preterm delivery are associated with Ureaplasma infection. We hypothesized that Ureaplasma induced extracellular collagen fragmentation results in production of the tripeptide PGP (proline-glycine-proline), a neutrophil chemoattractant. PGP release from...

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Autores principales: Lal, Charitharth V., Xu, Xin, Jackson, Patricia, Atkinson, Thomas P., Faye-Petersen, Ona M., Kandasamy, Jegen, Waites, Ken, Biggio, Joseph R., Gaggar, Amit, Ambalavanan, Namasivayam
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5235960/
https://www.ncbi.nlm.nih.gov/pubmed/27632777
http://dx.doi.org/10.1038/pr.2016.176
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author Lal, Charitharth V.
Xu, Xin
Jackson, Patricia
Atkinson, Thomas P.
Faye-Petersen, Ona M.
Kandasamy, Jegen
Waites, Ken
Biggio, Joseph R.
Gaggar, Amit
Ambalavanan, Namasivayam
author_facet Lal, Charitharth V.
Xu, Xin
Jackson, Patricia
Atkinson, Thomas P.
Faye-Petersen, Ona M.
Kandasamy, Jegen
Waites, Ken
Biggio, Joseph R.
Gaggar, Amit
Ambalavanan, Namasivayam
author_sort Lal, Charitharth V.
collection PubMed
description BACKGROUND: Premature rupture of membranes and preterm delivery are associated with Ureaplasma infection. We hypothesized that Ureaplasma induced extracellular collagen fragmentation results in production of the tripeptide PGP (proline-glycine-proline), a neutrophil chemoattractant. PGP release from collagen requires matrix metalloproteases (MMP-8/MMP-9) along with a serine protease, prolyl endopeptidase (PE). METHODS: Ureaplasma culture negative amniotic fluid (indicated preterm birth, n=8; spontaneous preterm birth, n=8) and Ureaplasma positive amniotic fluid (spontaneous preterm birth, n=8) were analyzed by electro-spray ionization-liquid chromatography tandem mass spectrometry for PGP, and for MMP-9 by zymography. PE was evaluated in lysates of U. parvum serovar 3 (Up3) and U. urealyticum serovar 10 (Uu10) by western blotting and activity assay. RESULTS: PGP and MMP-9 were increased in amniotic fluid from spontaneous preterm birth with positive Ureaplasma cultures, but not with indicated preterm birth or spontaneous preterm birth with negative Ureaplasma cultures. Human neutrophils co-cultured with Ureaplasma strains showed increased MMP-9 activity. PE presence and activity were noted with both Ureaplasma strains. CONCLUSIONS: Ureaplasma spp. carry the protease necessary for PGP release, and PGP and MMP-9 are increased in amniotic fluid during Ureaplasma infection, suggesting Ureaplasma spp. induced collagen fragmentation contributes to preterm rupture of membranes and neutrophil influx causing chorioamnionitis.
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spelling pubmed-52359602017-03-15 Ureaplasma Infection Mediated Release of Matrix Metalloproteinase-9 and PGP – a Novel Mechanism of Preterm Rupture of Membranes and Chorioamnionitis Lal, Charitharth V. Xu, Xin Jackson, Patricia Atkinson, Thomas P. Faye-Petersen, Ona M. Kandasamy, Jegen Waites, Ken Biggio, Joseph R. Gaggar, Amit Ambalavanan, Namasivayam Pediatr Res Article BACKGROUND: Premature rupture of membranes and preterm delivery are associated with Ureaplasma infection. We hypothesized that Ureaplasma induced extracellular collagen fragmentation results in production of the tripeptide PGP (proline-glycine-proline), a neutrophil chemoattractant. PGP release from collagen requires matrix metalloproteases (MMP-8/MMP-9) along with a serine protease, prolyl endopeptidase (PE). METHODS: Ureaplasma culture negative amniotic fluid (indicated preterm birth, n=8; spontaneous preterm birth, n=8) and Ureaplasma positive amniotic fluid (spontaneous preterm birth, n=8) were analyzed by electro-spray ionization-liquid chromatography tandem mass spectrometry for PGP, and for MMP-9 by zymography. PE was evaluated in lysates of U. parvum serovar 3 (Up3) and U. urealyticum serovar 10 (Uu10) by western blotting and activity assay. RESULTS: PGP and MMP-9 were increased in amniotic fluid from spontaneous preterm birth with positive Ureaplasma cultures, but not with indicated preterm birth or spontaneous preterm birth with negative Ureaplasma cultures. Human neutrophils co-cultured with Ureaplasma strains showed increased MMP-9 activity. PE presence and activity were noted with both Ureaplasma strains. CONCLUSIONS: Ureaplasma spp. carry the protease necessary for PGP release, and PGP and MMP-9 are increased in amniotic fluid during Ureaplasma infection, suggesting Ureaplasma spp. induced collagen fragmentation contributes to preterm rupture of membranes and neutrophil influx causing chorioamnionitis. 2016-09-15 2017-01 /pmc/articles/PMC5235960/ /pubmed/27632777 http://dx.doi.org/10.1038/pr.2016.176 Text en http://www.nature.com/authors/editorial_policies/license.html#terms Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Lal, Charitharth V.
Xu, Xin
Jackson, Patricia
Atkinson, Thomas P.
Faye-Petersen, Ona M.
Kandasamy, Jegen
Waites, Ken
Biggio, Joseph R.
Gaggar, Amit
Ambalavanan, Namasivayam
Ureaplasma Infection Mediated Release of Matrix Metalloproteinase-9 and PGP – a Novel Mechanism of Preterm Rupture of Membranes and Chorioamnionitis
title Ureaplasma Infection Mediated Release of Matrix Metalloproteinase-9 and PGP – a Novel Mechanism of Preterm Rupture of Membranes and Chorioamnionitis
title_full Ureaplasma Infection Mediated Release of Matrix Metalloproteinase-9 and PGP – a Novel Mechanism of Preterm Rupture of Membranes and Chorioamnionitis
title_fullStr Ureaplasma Infection Mediated Release of Matrix Metalloproteinase-9 and PGP – a Novel Mechanism of Preterm Rupture of Membranes and Chorioamnionitis
title_full_unstemmed Ureaplasma Infection Mediated Release of Matrix Metalloproteinase-9 and PGP – a Novel Mechanism of Preterm Rupture of Membranes and Chorioamnionitis
title_short Ureaplasma Infection Mediated Release of Matrix Metalloproteinase-9 and PGP – a Novel Mechanism of Preterm Rupture of Membranes and Chorioamnionitis
title_sort ureaplasma infection mediated release of matrix metalloproteinase-9 and pgp – a novel mechanism of preterm rupture of membranes and chorioamnionitis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5235960/
https://www.ncbi.nlm.nih.gov/pubmed/27632777
http://dx.doi.org/10.1038/pr.2016.176
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