Online flow cytometry, an interesting investigation process for monitoring lipid accumulation, dimorphism, and cells’ growth in the oleaginous yeast Yarrowia lipolytica JMY 775

This study aims to understand and better control the main biological mechanisms and parameters modulating the various phenomena affecting Yarrowia lipolytica JMY775 and its lipids accumulation. The results obtained in this study stress forward that the use of an original tool, consisting of coupling...

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Detalles Bibliográficos
Autores principales: Bouchedja, Doria Naila, Danthine, Sabine, Kar, Tambi, Fickers, Patrick, Boudjellal, Abdelghani, Delvigne, Frank
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2017
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5236074/
https://www.ncbi.nlm.nih.gov/pubmed/28133594
http://dx.doi.org/10.1186/s40643-016-0132-6
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author Bouchedja, Doria Naila
Danthine, Sabine
Kar, Tambi
Fickers, Patrick
Boudjellal, Abdelghani
Delvigne, Frank
author_facet Bouchedja, Doria Naila
Danthine, Sabine
Kar, Tambi
Fickers, Patrick
Boudjellal, Abdelghani
Delvigne, Frank
author_sort Bouchedja, Doria Naila
collection PubMed
description This study aims to understand and better control the main biological mechanisms and parameters modulating the various phenomena affecting Yarrowia lipolytica JMY775 and its lipids accumulation. The results obtained in this study stress forward that the use of an original tool, consisting of coupling bioreactors to online flow cytometry, is highly efficient. Throughout 48 h of culturing, this emerging process allowed an online continuous observation of the effects of pH and/or aeration on the cell growth and dimorphism and lipid accumulation by Y. lipolytica. This present study showed clearly that online flow cytometry is an advantageous tool for the real-time monitoring of microbial culture at a single-cell level. Indeed, the present investigation showed for the first time that profiling of the various phenomena and their monitoring upon culture time is now possible by coupling online cytometry with culture bioreactors.
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spelling pubmed-52360742017-01-25 Online flow cytometry, an interesting investigation process for monitoring lipid accumulation, dimorphism, and cells’ growth in the oleaginous yeast Yarrowia lipolytica JMY 775 Bouchedja, Doria Naila Danthine, Sabine Kar, Tambi Fickers, Patrick Boudjellal, Abdelghani Delvigne, Frank Bioresour Bioprocess Research This study aims to understand and better control the main biological mechanisms and parameters modulating the various phenomena affecting Yarrowia lipolytica JMY775 and its lipids accumulation. The results obtained in this study stress forward that the use of an original tool, consisting of coupling bioreactors to online flow cytometry, is highly efficient. Throughout 48 h of culturing, this emerging process allowed an online continuous observation of the effects of pH and/or aeration on the cell growth and dimorphism and lipid accumulation by Y. lipolytica. This present study showed clearly that online flow cytometry is an advantageous tool for the real-time monitoring of microbial culture at a single-cell level. Indeed, the present investigation showed for the first time that profiling of the various phenomena and their monitoring upon culture time is now possible by coupling online cytometry with culture bioreactors. Springer Berlin Heidelberg 2017-01-03 2017 /pmc/articles/PMC5236074/ /pubmed/28133594 http://dx.doi.org/10.1186/s40643-016-0132-6 Text en © The Author(s) 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Research
Bouchedja, Doria Naila
Danthine, Sabine
Kar, Tambi
Fickers, Patrick
Boudjellal, Abdelghani
Delvigne, Frank
Online flow cytometry, an interesting investigation process for monitoring lipid accumulation, dimorphism, and cells’ growth in the oleaginous yeast Yarrowia lipolytica JMY 775
title Online flow cytometry, an interesting investigation process for monitoring lipid accumulation, dimorphism, and cells’ growth in the oleaginous yeast Yarrowia lipolytica JMY 775
title_full Online flow cytometry, an interesting investigation process for monitoring lipid accumulation, dimorphism, and cells’ growth in the oleaginous yeast Yarrowia lipolytica JMY 775
title_fullStr Online flow cytometry, an interesting investigation process for monitoring lipid accumulation, dimorphism, and cells’ growth in the oleaginous yeast Yarrowia lipolytica JMY 775
title_full_unstemmed Online flow cytometry, an interesting investigation process for monitoring lipid accumulation, dimorphism, and cells’ growth in the oleaginous yeast Yarrowia lipolytica JMY 775
title_short Online flow cytometry, an interesting investigation process for monitoring lipid accumulation, dimorphism, and cells’ growth in the oleaginous yeast Yarrowia lipolytica JMY 775
title_sort online flow cytometry, an interesting investigation process for monitoring lipid accumulation, dimorphism, and cells’ growth in the oleaginous yeast yarrowia lipolytica jmy 775
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5236074/
https://www.ncbi.nlm.nih.gov/pubmed/28133594
http://dx.doi.org/10.1186/s40643-016-0132-6
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