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Echinococcus granulosus Sensu Stricto in Dogs and Jackals from Caspian Sea Region, Northern Iran

BACKGROUND: The aim of the present study was genotyping of Echinococcus granulosus isolates from dogs and jackals in Mazandaran Province, northern Iran, and using partial sequence of the mitochondrial cytochrome c oxidase subunit 1 gene (cox1). METHODS: E. granulosus isolates (n = 15) were collected...

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Autores principales: GHOLAMI, Shirzad, JAHANDAR, Hefzallah, ABASTABAR, Mahdi, PAGHEH, Abdolsatar, MOBEDI, Iraj, SHARBATKHORI, Mitra
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Tehran University of Medical Sciences 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5236095/
https://www.ncbi.nlm.nih.gov/pubmed/28096852
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author GHOLAMI, Shirzad
JAHANDAR, Hefzallah
ABASTABAR, Mahdi
PAGHEH, Abdolsatar
MOBEDI, Iraj
SHARBATKHORI, Mitra
author_facet GHOLAMI, Shirzad
JAHANDAR, Hefzallah
ABASTABAR, Mahdi
PAGHEH, Abdolsatar
MOBEDI, Iraj
SHARBATKHORI, Mitra
author_sort GHOLAMI, Shirzad
collection PubMed
description BACKGROUND: The aim of the present study was genotyping of Echinococcus granulosus isolates from dogs and jackals in Mazandaran Province, northern Iran, and using partial sequence of the mitochondrial cytochrome c oxidase subunit 1 gene (cox1). METHODS: E. granulosus isolates (n = 15) were collected from 42 stray dogs and 16 jackals found in south of the Caspian Sea in northern Iran. After morphological study, the isolates were genetically characterized using consensus sequences (366bp) of the cox1 gene. Phylogenetic analysis of cox1 nucleotide sequence data was performed using a Bayesian Inference approach. RESULTS: Four different sequences were observed among the isolates. Two genotypes [G1 (66.7%) and G3 (33.3%)] were identified among the isolates. The G1 sequences indicated three sequence profiles. One profile (Maz1) had 100% homology with reference sequence (AN: KP339045). Two other profiles, designated Maz2 and Maz3, had 99% homology with the G1 genotype (ANs: KP339046 and KP339047). A G3 sequence designated Maz4 showed 100% homology with a G3 reference sequence (AN: KP339048). CONCLUSION: The occurrence of the G1 genotype of E. granulosus sensu stricto as a frequent genotype in dogs is emphasized. This study established the first molecular characterization of E. granulosus in the province.
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spelling pubmed-52360952017-01-17 Echinococcus granulosus Sensu Stricto in Dogs and Jackals from Caspian Sea Region, Northern Iran GHOLAMI, Shirzad JAHANDAR, Hefzallah ABASTABAR, Mahdi PAGHEH, Abdolsatar MOBEDI, Iraj SHARBATKHORI, Mitra Iran J Parasitol Original Article BACKGROUND: The aim of the present study was genotyping of Echinococcus granulosus isolates from dogs and jackals in Mazandaran Province, northern Iran, and using partial sequence of the mitochondrial cytochrome c oxidase subunit 1 gene (cox1). METHODS: E. granulosus isolates (n = 15) were collected from 42 stray dogs and 16 jackals found in south of the Caspian Sea in northern Iran. After morphological study, the isolates were genetically characterized using consensus sequences (366bp) of the cox1 gene. Phylogenetic analysis of cox1 nucleotide sequence data was performed using a Bayesian Inference approach. RESULTS: Four different sequences were observed among the isolates. Two genotypes [G1 (66.7%) and G3 (33.3%)] were identified among the isolates. The G1 sequences indicated three sequence profiles. One profile (Maz1) had 100% homology with reference sequence (AN: KP339045). Two other profiles, designated Maz2 and Maz3, had 99% homology with the G1 genotype (ANs: KP339046 and KP339047). A G3 sequence designated Maz4 showed 100% homology with a G3 reference sequence (AN: KP339048). CONCLUSION: The occurrence of the G1 genotype of E. granulosus sensu stricto as a frequent genotype in dogs is emphasized. This study established the first molecular characterization of E. granulosus in the province. Tehran University of Medical Sciences 2016 /pmc/articles/PMC5236095/ /pubmed/28096852 Text en Copyright© Iranian Society of Parasitology & Tehran University of Medical Sciences This work is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License which allows users to read, copy, distribute and make derivative works for non-commercial purposes from the material, as long as the author of the original work is cited properly.
spellingShingle Original Article
GHOLAMI, Shirzad
JAHANDAR, Hefzallah
ABASTABAR, Mahdi
PAGHEH, Abdolsatar
MOBEDI, Iraj
SHARBATKHORI, Mitra
Echinococcus granulosus Sensu Stricto in Dogs and Jackals from Caspian Sea Region, Northern Iran
title Echinococcus granulosus Sensu Stricto in Dogs and Jackals from Caspian Sea Region, Northern Iran
title_full Echinococcus granulosus Sensu Stricto in Dogs and Jackals from Caspian Sea Region, Northern Iran
title_fullStr Echinococcus granulosus Sensu Stricto in Dogs and Jackals from Caspian Sea Region, Northern Iran
title_full_unstemmed Echinococcus granulosus Sensu Stricto in Dogs and Jackals from Caspian Sea Region, Northern Iran
title_short Echinococcus granulosus Sensu Stricto in Dogs and Jackals from Caspian Sea Region, Northern Iran
title_sort echinococcus granulosus sensu stricto in dogs and jackals from caspian sea region, northern iran
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5236095/
https://www.ncbi.nlm.nih.gov/pubmed/28096852
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