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Methicillin resistance and virulence genes in invasive and nasal Staphylococcus epidermidis isolates from neonates

BACKGROUND: Staphylococcus epidermidis is an opportunistic pathogen involved in hospital-acquired infections, particularly in those related to medical devices. This study characterized 50 genetically unrelated S. epidermidis isolates from bloodstream infections (BSIs, n = 31) and nares (n = 19) of n...

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Autores principales: Salgueiro, Vivian Carolina, Iorio, Natalia Lopes Pontes, Ferreira, Marcelle Cristina, Chamon, Raiane Cardoso, dos Santos, Kátia Regina Netto
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5237318/
https://www.ncbi.nlm.nih.gov/pubmed/28086793
http://dx.doi.org/10.1186/s12866-017-0930-9
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author Salgueiro, Vivian Carolina
Iorio, Natalia Lopes Pontes
Ferreira, Marcelle Cristina
Chamon, Raiane Cardoso
dos Santos, Kátia Regina Netto
author_facet Salgueiro, Vivian Carolina
Iorio, Natalia Lopes Pontes
Ferreira, Marcelle Cristina
Chamon, Raiane Cardoso
dos Santos, Kátia Regina Netto
author_sort Salgueiro, Vivian Carolina
collection PubMed
description BACKGROUND: Staphylococcus epidermidis is an opportunistic pathogen involved in hospital-acquired infections, particularly in those related to medical devices. This study characterized 50 genetically unrelated S. epidermidis isolates from bloodstream infections (BSIs, n = 31) and nares (n = 19) of neonates in relation to staphylococcal chromosomal cassette mec (SCCmec) type, biofilm production and associated genes, and the arginine catabolic mobile elements (ACME), in order to detect virulence factors that could discriminate a potential invasiveness isolate or predict an increasing pathogenicity. RESULTS: Isolates from both groups showed no difference for biofilm production and ACME genes detection. However, BSI isolates harbored more frequently the sdrF and sesI genes (p < 0.05), whereas biofilm producer isolates were associated with presence of the aap gene. The sdrF gene was also significantly more in the biofilm producer isolates from BSI. The SCCmec type IV and the ccr2 complex were related to BSI isolates (p < 0.05), while 83% of the nasal isolates were non-typeable for the SCCmec elements, with the mec complex and ccr undetectable as the most frequent profile. CONCLUSIONS: Despite the great clonal diversity displayed by S. epidermidis isolates from neonates, BSI isolates harbored more frequently the sdrF and sesI adhesin genes, while nasal isolates were very variable in SCCmec composition. These aspects could be advantageous to improve colonization in the host increasing its pathogenicity.
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spelling pubmed-52373182017-01-18 Methicillin resistance and virulence genes in invasive and nasal Staphylococcus epidermidis isolates from neonates Salgueiro, Vivian Carolina Iorio, Natalia Lopes Pontes Ferreira, Marcelle Cristina Chamon, Raiane Cardoso dos Santos, Kátia Regina Netto BMC Microbiol Research Article BACKGROUND: Staphylococcus epidermidis is an opportunistic pathogen involved in hospital-acquired infections, particularly in those related to medical devices. This study characterized 50 genetically unrelated S. epidermidis isolates from bloodstream infections (BSIs, n = 31) and nares (n = 19) of neonates in relation to staphylococcal chromosomal cassette mec (SCCmec) type, biofilm production and associated genes, and the arginine catabolic mobile elements (ACME), in order to detect virulence factors that could discriminate a potential invasiveness isolate or predict an increasing pathogenicity. RESULTS: Isolates from both groups showed no difference for biofilm production and ACME genes detection. However, BSI isolates harbored more frequently the sdrF and sesI genes (p < 0.05), whereas biofilm producer isolates were associated with presence of the aap gene. The sdrF gene was also significantly more in the biofilm producer isolates from BSI. The SCCmec type IV and the ccr2 complex were related to BSI isolates (p < 0.05), while 83% of the nasal isolates were non-typeable for the SCCmec elements, with the mec complex and ccr undetectable as the most frequent profile. CONCLUSIONS: Despite the great clonal diversity displayed by S. epidermidis isolates from neonates, BSI isolates harbored more frequently the sdrF and sesI adhesin genes, while nasal isolates were very variable in SCCmec composition. These aspects could be advantageous to improve colonization in the host increasing its pathogenicity. BioMed Central 2017-01-13 /pmc/articles/PMC5237318/ /pubmed/28086793 http://dx.doi.org/10.1186/s12866-017-0930-9 Text en © The Author(s). 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Salgueiro, Vivian Carolina
Iorio, Natalia Lopes Pontes
Ferreira, Marcelle Cristina
Chamon, Raiane Cardoso
dos Santos, Kátia Regina Netto
Methicillin resistance and virulence genes in invasive and nasal Staphylococcus epidermidis isolates from neonates
title Methicillin resistance and virulence genes in invasive and nasal Staphylococcus epidermidis isolates from neonates
title_full Methicillin resistance and virulence genes in invasive and nasal Staphylococcus epidermidis isolates from neonates
title_fullStr Methicillin resistance and virulence genes in invasive and nasal Staphylococcus epidermidis isolates from neonates
title_full_unstemmed Methicillin resistance and virulence genes in invasive and nasal Staphylococcus epidermidis isolates from neonates
title_short Methicillin resistance and virulence genes in invasive and nasal Staphylococcus epidermidis isolates from neonates
title_sort methicillin resistance and virulence genes in invasive and nasal staphylococcus epidermidis isolates from neonates
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5237318/
https://www.ncbi.nlm.nih.gov/pubmed/28086793
http://dx.doi.org/10.1186/s12866-017-0930-9
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