Cargando…

Transcriptional profiles in bursal B-lymphoid DT40 cells infected with very virulent infectious bursal disease virus

BACKGROUND: Infectious bursal disease virus (IBDV) causes a highly contagious, immunosuppressive disease in chickens. The virus mainly infects immature B lymphocytes in the bursa of Fabricius (BF). Chicken B cell line DT40, an avian leukosis virus-induced B cell line, supports very virulent IBDV (vv...

Descripción completa

Detalles Bibliográficos
Autores principales: Quan, Rong, Zhu, Shanshan, Wei, Li, Wang, Jing, Yan, Xu, Li, Zixuan, Liu, Jue
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5237357/
https://www.ncbi.nlm.nih.gov/pubmed/28086922
http://dx.doi.org/10.1186/s12985-016-0668-2
_version_ 1782495520758956032
author Quan, Rong
Zhu, Shanshan
Wei, Li
Wang, Jing
Yan, Xu
Li, Zixuan
Liu, Jue
author_facet Quan, Rong
Zhu, Shanshan
Wei, Li
Wang, Jing
Yan, Xu
Li, Zixuan
Liu, Jue
author_sort Quan, Rong
collection PubMed
description BACKGROUND: Infectious bursal disease virus (IBDV) causes a highly contagious, immunosuppressive disease in chickens. The virus mainly infects immature B lymphocytes in the bursa of Fabricius (BF). Chicken B cell line DT40, an avian leukosis virus-induced B cell line, supports very virulent IBDV (vvIBDV) infection in vitro and thereby serves as a good model for investigating the infection and pathogenesis of this virus. However, a transcriptome-wide understanding of the interaction between vvIBDV and B cells has not yet been achieved. This study aimed to employ time-course DNA microarrays to investigate gene expression patterns in DT40 cells after infection with vvIBDV strain LX. RESULTS: DT40 cells infected with vvIBDV exhibited alterations in the expression of many important host genes involved in signal transduction pathways, including MAPK signaling, PI3K/mTOR signaling, cell death and survival, BCR signaling, and antigen presentation. The changes in cellular mRNA levels identified by microarray analysis were confirmed for 8 selected genes using real-time reverse transcription-PCR. The upregulation of inflammatory cytokines and Toll-like receptors (TLRs) in the bursa of vvIBDV-infected chickens might involve excessive activation of the innate immune and inflammatory responses and contribute to tissue damage. CONCLUSIONS: The present study is the first to provide a comprehensive differential transcriptional profile of cultured DT40 cells in response to vvIBDV infection and further extends our understanding of the molecular mechanisms underlying vvIBDV infection and pathogenesis.
format Online
Article
Text
id pubmed-5237357
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-52373572017-01-18 Transcriptional profiles in bursal B-lymphoid DT40 cells infected with very virulent infectious bursal disease virus Quan, Rong Zhu, Shanshan Wei, Li Wang, Jing Yan, Xu Li, Zixuan Liu, Jue Virol J Research BACKGROUND: Infectious bursal disease virus (IBDV) causes a highly contagious, immunosuppressive disease in chickens. The virus mainly infects immature B lymphocytes in the bursa of Fabricius (BF). Chicken B cell line DT40, an avian leukosis virus-induced B cell line, supports very virulent IBDV (vvIBDV) infection in vitro and thereby serves as a good model for investigating the infection and pathogenesis of this virus. However, a transcriptome-wide understanding of the interaction between vvIBDV and B cells has not yet been achieved. This study aimed to employ time-course DNA microarrays to investigate gene expression patterns in DT40 cells after infection with vvIBDV strain LX. RESULTS: DT40 cells infected with vvIBDV exhibited alterations in the expression of many important host genes involved in signal transduction pathways, including MAPK signaling, PI3K/mTOR signaling, cell death and survival, BCR signaling, and antigen presentation. The changes in cellular mRNA levels identified by microarray analysis were confirmed for 8 selected genes using real-time reverse transcription-PCR. The upregulation of inflammatory cytokines and Toll-like receptors (TLRs) in the bursa of vvIBDV-infected chickens might involve excessive activation of the innate immune and inflammatory responses and contribute to tissue damage. CONCLUSIONS: The present study is the first to provide a comprehensive differential transcriptional profile of cultured DT40 cells in response to vvIBDV infection and further extends our understanding of the molecular mechanisms underlying vvIBDV infection and pathogenesis. BioMed Central 2017-01-13 /pmc/articles/PMC5237357/ /pubmed/28086922 http://dx.doi.org/10.1186/s12985-016-0668-2 Text en © The Author(s). 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Quan, Rong
Zhu, Shanshan
Wei, Li
Wang, Jing
Yan, Xu
Li, Zixuan
Liu, Jue
Transcriptional profiles in bursal B-lymphoid DT40 cells infected with very virulent infectious bursal disease virus
title Transcriptional profiles in bursal B-lymphoid DT40 cells infected with very virulent infectious bursal disease virus
title_full Transcriptional profiles in bursal B-lymphoid DT40 cells infected with very virulent infectious bursal disease virus
title_fullStr Transcriptional profiles in bursal B-lymphoid DT40 cells infected with very virulent infectious bursal disease virus
title_full_unstemmed Transcriptional profiles in bursal B-lymphoid DT40 cells infected with very virulent infectious bursal disease virus
title_short Transcriptional profiles in bursal B-lymphoid DT40 cells infected with very virulent infectious bursal disease virus
title_sort transcriptional profiles in bursal b-lymphoid dt40 cells infected with very virulent infectious bursal disease virus
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5237357/
https://www.ncbi.nlm.nih.gov/pubmed/28086922
http://dx.doi.org/10.1186/s12985-016-0668-2
work_keys_str_mv AT quanrong transcriptionalprofilesinbursalblymphoiddt40cellsinfectedwithveryvirulentinfectiousbursaldiseasevirus
AT zhushanshan transcriptionalprofilesinbursalblymphoiddt40cellsinfectedwithveryvirulentinfectiousbursaldiseasevirus
AT weili transcriptionalprofilesinbursalblymphoiddt40cellsinfectedwithveryvirulentinfectiousbursaldiseasevirus
AT wangjing transcriptionalprofilesinbursalblymphoiddt40cellsinfectedwithveryvirulentinfectiousbursaldiseasevirus
AT yanxu transcriptionalprofilesinbursalblymphoiddt40cellsinfectedwithveryvirulentinfectiousbursaldiseasevirus
AT lizixuan transcriptionalprofilesinbursalblymphoiddt40cellsinfectedwithveryvirulentinfectiousbursaldiseasevirus
AT liujue transcriptionalprofilesinbursalblymphoiddt40cellsinfectedwithveryvirulentinfectiousbursaldiseasevirus