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The antibacterial activity and mechanism of ginkgolic acid C15:1
BACKGROUND: The present study investigated the antibacterial activity and underlying mechanisms of ginkgolic acid (GA) C15:1 monomer using green fluorescent protein (GFP)-labeled bacteria strains. RESULTS: GA presented significant antibacterial activity against Gram-positive bacteria but generally d...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5237537/ https://www.ncbi.nlm.nih.gov/pubmed/28088196 http://dx.doi.org/10.1186/s12896-016-0324-3 |
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author | Hua, Zhebin Wu, Caie Fan, Gongjian Tang, Zhenxing Cao, Fuliang |
author_facet | Hua, Zhebin Wu, Caie Fan, Gongjian Tang, Zhenxing Cao, Fuliang |
author_sort | Hua, Zhebin |
collection | PubMed |
description | BACKGROUND: The present study investigated the antibacterial activity and underlying mechanisms of ginkgolic acid (GA) C15:1 monomer using green fluorescent protein (GFP)-labeled bacteria strains. RESULTS: GA presented significant antibacterial activity against Gram-positive bacteria but generally did not affect the growth of Gram-negative bacteria. The studies of the antibacterial mechanism indicated that large amounts of GA (C15:1) could penetrate GFP-labeled Bacillus amyloliquefaciens in a short period of time, and as a result, led to the quenching of GFP in bacteria. In vitro results demonstrated that GA (C15:1) could inhibit the activity of multiple proteins including DNA polymerase. In vivo results showed that GA (C15:1) could significantly inhibit the biosynthesis of DNA, RNA and B. amyloliquefaciens proteins. CONCLUSION: We speculated that GA (C15:1) achieved its antibacterial effect through inhibiting the protein activity of B. amyloliquefaciens. GA (C15:1) could not penetrate Gram-negative bacteria in large amounts, and the lipid soluble components in the bacterial cell wall could intercept GA (C15:1), which was one of the primary reasons that GA (C15:1) did not have a significant antibacterial effect on Gram-negative bacteria. |
format | Online Article Text |
id | pubmed-5237537 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-52375372017-01-18 The antibacterial activity and mechanism of ginkgolic acid C15:1 Hua, Zhebin Wu, Caie Fan, Gongjian Tang, Zhenxing Cao, Fuliang BMC Biotechnol Research Article BACKGROUND: The present study investigated the antibacterial activity and underlying mechanisms of ginkgolic acid (GA) C15:1 monomer using green fluorescent protein (GFP)-labeled bacteria strains. RESULTS: GA presented significant antibacterial activity against Gram-positive bacteria but generally did not affect the growth of Gram-negative bacteria. The studies of the antibacterial mechanism indicated that large amounts of GA (C15:1) could penetrate GFP-labeled Bacillus amyloliquefaciens in a short period of time, and as a result, led to the quenching of GFP in bacteria. In vitro results demonstrated that GA (C15:1) could inhibit the activity of multiple proteins including DNA polymerase. In vivo results showed that GA (C15:1) could significantly inhibit the biosynthesis of DNA, RNA and B. amyloliquefaciens proteins. CONCLUSION: We speculated that GA (C15:1) achieved its antibacterial effect through inhibiting the protein activity of B. amyloliquefaciens. GA (C15:1) could not penetrate Gram-negative bacteria in large amounts, and the lipid soluble components in the bacterial cell wall could intercept GA (C15:1), which was one of the primary reasons that GA (C15:1) did not have a significant antibacterial effect on Gram-negative bacteria. BioMed Central 2017-01-14 /pmc/articles/PMC5237537/ /pubmed/28088196 http://dx.doi.org/10.1186/s12896-016-0324-3 Text en © The Author(s). 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Hua, Zhebin Wu, Caie Fan, Gongjian Tang, Zhenxing Cao, Fuliang The antibacterial activity and mechanism of ginkgolic acid C15:1 |
title | The antibacterial activity and mechanism of ginkgolic acid C15:1 |
title_full | The antibacterial activity and mechanism of ginkgolic acid C15:1 |
title_fullStr | The antibacterial activity and mechanism of ginkgolic acid C15:1 |
title_full_unstemmed | The antibacterial activity and mechanism of ginkgolic acid C15:1 |
title_short | The antibacterial activity and mechanism of ginkgolic acid C15:1 |
title_sort | antibacterial activity and mechanism of ginkgolic acid c15:1 |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5237537/ https://www.ncbi.nlm.nih.gov/pubmed/28088196 http://dx.doi.org/10.1186/s12896-016-0324-3 |
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