Development of a Novel Quantum Dots and Graphene Oxide Based FRET Assay for Rapid Detection of invA Gene of Salmonella

A novel, rapid and simple fluorescence resonance energy transfer (FRET) based Salmonella specific gene, invA, detection system was developed, in which quantum dots (QDs) and graphene oxide (GO) worked as fluorescent donor and quencher, respectively. By measuring the fluorescence intensity signal, th...

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Autores principales: Guo, Jiubiao, Chan, Edward W. C., Chen, Sheng, Zeng, Zhenling
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2017
Materias:
Microbiology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5239778/
https://www.ncbi.nlm.nih.gov/pubmed/28144237
http://dx.doi.org/10.3389/fmicb.2017.00008
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author Guo, Jiubiao
Chan, Edward W. C.
Chen, Sheng
Zeng, Zhenling
author_facet Guo, Jiubiao
Chan, Edward W. C.
Chen, Sheng
Zeng, Zhenling
author_sort Guo, Jiubiao
collection PubMed
description A novel, rapid and simple fluorescence resonance energy transfer (FRET) based Salmonella specific gene, invA, detection system was developed, in which quantum dots (QDs) and graphene oxide (GO) worked as fluorescent donor and quencher, respectively. By measuring the fluorescence intensity signal, the Salmonella specific invA gene could be sensitively and specifically detected with a limit of detection (LOD) of ∼4 nM of the invA gene in 20 min. The developed system has the potential to be used for Salmonella detection in food and environmental samples and further developed into a platform for detection of other bacterial pathogens.
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spelling pubmed-52397782017-01-31 Development of a Novel Quantum Dots and Graphene Oxide Based FRET Assay for Rapid Detection of invA Gene of Salmonella Guo, Jiubiao Chan, Edward W. C. Chen, Sheng Zeng, Zhenling Front Microbiol Microbiology A novel, rapid and simple fluorescence resonance energy transfer (FRET) based Salmonella specific gene, invA, detection system was developed, in which quantum dots (QDs) and graphene oxide (GO) worked as fluorescent donor and quencher, respectively. By measuring the fluorescence intensity signal, the Salmonella specific invA gene could be sensitively and specifically detected with a limit of detection (LOD) of ∼4 nM of the invA gene in 20 min. The developed system has the potential to be used for Salmonella detection in food and environmental samples and further developed into a platform for detection of other bacterial pathogens. Frontiers Media S.A. 2017-01-17 /pmc/articles/PMC5239778/ /pubmed/28144237 http://dx.doi.org/10.3389/fmicb.2017.00008 Text en Copyright © 2017 Guo, Chan, Chen and Zeng. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Guo, Jiubiao
Chan, Edward W. C.
Chen, Sheng
Zeng, Zhenling
Development of a Novel Quantum Dots and Graphene Oxide Based FRET Assay for Rapid Detection of invA Gene of Salmonella
title Development of a Novel Quantum Dots and Graphene Oxide Based FRET Assay for Rapid Detection of invA Gene of Salmonella
title_full Development of a Novel Quantum Dots and Graphene Oxide Based FRET Assay for Rapid Detection of invA Gene of Salmonella
title_fullStr Development of a Novel Quantum Dots and Graphene Oxide Based FRET Assay for Rapid Detection of invA Gene of Salmonella
title_full_unstemmed Development of a Novel Quantum Dots and Graphene Oxide Based FRET Assay for Rapid Detection of invA Gene of Salmonella
title_short Development of a Novel Quantum Dots and Graphene Oxide Based FRET Assay for Rapid Detection of invA Gene of Salmonella
title_sort development of a novel quantum dots and graphene oxide based fret assay for rapid detection of inva gene of salmonella
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5239778/
https://www.ncbi.nlm.nih.gov/pubmed/28144237
http://dx.doi.org/10.3389/fmicb.2017.00008
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