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Anastatica hierochuntica, an Arabidopsis Desert Relative, Is Tolerant to Multiple Abiotic Stresses and Exhibits Species-Specific and Common Stress Tolerance Strategies with Its Halophytic Relative, Eutrema (Thellungiella) salsugineum

The search for novel stress tolerance determinants has led to increasing interest in plants native to extreme environments – so called “extremophytes.” One successful strategy has been comparative studies between Arabidopsis thaliana and extremophyte Brassicaceae relatives such as the halophyte Eutr...

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Autores principales: Eshel, Gil, Shaked, Ruth, Kazachkova, Yana, Khan, Asif, Eppel, Amir, Cisneros, Aroldo, Acuna, Tania, Gutterman, Yitzhak, Tel-Zur, Noemi, Rachmilevitch, Shimon, Fait, Aaron, Barak, Simon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5239783/
https://www.ncbi.nlm.nih.gov/pubmed/28144244
http://dx.doi.org/10.3389/fpls.2016.01992
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author Eshel, Gil
Shaked, Ruth
Kazachkova, Yana
Khan, Asif
Eppel, Amir
Cisneros, Aroldo
Acuna, Tania
Gutterman, Yitzhak
Tel-Zur, Noemi
Rachmilevitch, Shimon
Fait, Aaron
Barak, Simon
author_facet Eshel, Gil
Shaked, Ruth
Kazachkova, Yana
Khan, Asif
Eppel, Amir
Cisneros, Aroldo
Acuna, Tania
Gutterman, Yitzhak
Tel-Zur, Noemi
Rachmilevitch, Shimon
Fait, Aaron
Barak, Simon
author_sort Eshel, Gil
collection PubMed
description The search for novel stress tolerance determinants has led to increasing interest in plants native to extreme environments – so called “extremophytes.” One successful strategy has been comparative studies between Arabidopsis thaliana and extremophyte Brassicaceae relatives such as the halophyte Eutrema salsugineum located in areas including cold, salty coastal regions of China. Here, we investigate stress tolerance in the desert species, Anastatica hierochuntica (True Rose of Jericho), a member of the poorly investigated lineage III Brassicaceae. We show that A. hierochuntica has a genome approximately 4.5-fold larger than Arabidopsis, divided into 22 diploid chromosomes, and demonstrate that A. hierochuntica exhibits tolerance to heat, low N and salt stresses that are characteristic of its habitat. Taking salt tolerance as a case study, we show that A. hierochuntica shares common salt tolerance mechanisms with E. salsugineum such as tight control of shoot Na(+) accumulation and resilient photochemistry features. Furthermore, metabolic profiling of E. salsugineum and A. hierochuntica shoots demonstrates that the extremophytes exhibit both species-specific and common metabolic strategies to cope with salt stress including constitutive up-regulation (under control and salt stress conditions) of ascorbate and dehydroascorbate, two metabolites involved in ROS scavenging. Accordingly, A. hierochuntica displays tolerance to methyl viologen-induced oxidative stress suggesting that a highly active antioxidant system is essential to cope with multiple abiotic stresses. We suggest that A. hierochuntica presents an excellent extremophyte Arabidopsis relative model system for understanding plant survival in harsh desert conditions.
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spelling pubmed-52397832017-01-31 Anastatica hierochuntica, an Arabidopsis Desert Relative, Is Tolerant to Multiple Abiotic Stresses and Exhibits Species-Specific and Common Stress Tolerance Strategies with Its Halophytic Relative, Eutrema (Thellungiella) salsugineum Eshel, Gil Shaked, Ruth Kazachkova, Yana Khan, Asif Eppel, Amir Cisneros, Aroldo Acuna, Tania Gutterman, Yitzhak Tel-Zur, Noemi Rachmilevitch, Shimon Fait, Aaron Barak, Simon Front Plant Sci Plant Science The search for novel stress tolerance determinants has led to increasing interest in plants native to extreme environments – so called “extremophytes.” One successful strategy has been comparative studies between Arabidopsis thaliana and extremophyte Brassicaceae relatives such as the halophyte Eutrema salsugineum located in areas including cold, salty coastal regions of China. Here, we investigate stress tolerance in the desert species, Anastatica hierochuntica (True Rose of Jericho), a member of the poorly investigated lineage III Brassicaceae. We show that A. hierochuntica has a genome approximately 4.5-fold larger than Arabidopsis, divided into 22 diploid chromosomes, and demonstrate that A. hierochuntica exhibits tolerance to heat, low N and salt stresses that are characteristic of its habitat. Taking salt tolerance as a case study, we show that A. hierochuntica shares common salt tolerance mechanisms with E. salsugineum such as tight control of shoot Na(+) accumulation and resilient photochemistry features. Furthermore, metabolic profiling of E. salsugineum and A. hierochuntica shoots demonstrates that the extremophytes exhibit both species-specific and common metabolic strategies to cope with salt stress including constitutive up-regulation (under control and salt stress conditions) of ascorbate and dehydroascorbate, two metabolites involved in ROS scavenging. Accordingly, A. hierochuntica displays tolerance to methyl viologen-induced oxidative stress suggesting that a highly active antioxidant system is essential to cope with multiple abiotic stresses. We suggest that A. hierochuntica presents an excellent extremophyte Arabidopsis relative model system for understanding plant survival in harsh desert conditions. Frontiers Media S.A. 2017-01-17 /pmc/articles/PMC5239783/ /pubmed/28144244 http://dx.doi.org/10.3389/fpls.2016.01992 Text en Copyright © 2017 Eshel, Shaked, Kazachkova, Khan, Eppel, Cisneros, Acuna, Gutterman, Tel-Zur, Rachmilevitch, Fait and Barak. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Eshel, Gil
Shaked, Ruth
Kazachkova, Yana
Khan, Asif
Eppel, Amir
Cisneros, Aroldo
Acuna, Tania
Gutterman, Yitzhak
Tel-Zur, Noemi
Rachmilevitch, Shimon
Fait, Aaron
Barak, Simon
Anastatica hierochuntica, an Arabidopsis Desert Relative, Is Tolerant to Multiple Abiotic Stresses and Exhibits Species-Specific and Common Stress Tolerance Strategies with Its Halophytic Relative, Eutrema (Thellungiella) salsugineum
title Anastatica hierochuntica, an Arabidopsis Desert Relative, Is Tolerant to Multiple Abiotic Stresses and Exhibits Species-Specific and Common Stress Tolerance Strategies with Its Halophytic Relative, Eutrema (Thellungiella) salsugineum
title_full Anastatica hierochuntica, an Arabidopsis Desert Relative, Is Tolerant to Multiple Abiotic Stresses and Exhibits Species-Specific and Common Stress Tolerance Strategies with Its Halophytic Relative, Eutrema (Thellungiella) salsugineum
title_fullStr Anastatica hierochuntica, an Arabidopsis Desert Relative, Is Tolerant to Multiple Abiotic Stresses and Exhibits Species-Specific and Common Stress Tolerance Strategies with Its Halophytic Relative, Eutrema (Thellungiella) salsugineum
title_full_unstemmed Anastatica hierochuntica, an Arabidopsis Desert Relative, Is Tolerant to Multiple Abiotic Stresses and Exhibits Species-Specific and Common Stress Tolerance Strategies with Its Halophytic Relative, Eutrema (Thellungiella) salsugineum
title_short Anastatica hierochuntica, an Arabidopsis Desert Relative, Is Tolerant to Multiple Abiotic Stresses and Exhibits Species-Specific and Common Stress Tolerance Strategies with Its Halophytic Relative, Eutrema (Thellungiella) salsugineum
title_sort anastatica hierochuntica, an arabidopsis desert relative, is tolerant to multiple abiotic stresses and exhibits species-specific and common stress tolerance strategies with its halophytic relative, eutrema (thellungiella) salsugineum
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5239783/
https://www.ncbi.nlm.nih.gov/pubmed/28144244
http://dx.doi.org/10.3389/fpls.2016.01992
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