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Human Catestatin Alters Gut Microbiota Composition in Mice

The mammalian intestinal tract is heavily colonized with a dense, complex, and diversified microbial populations. In healthy individuals, an array of epithelial antimicrobial agents is secreted in the gut to aid intestinal homeostasis. Enterochromaffin cells (EC) in the intestinal epithelium are a m...

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Autores principales: Rabbi, Mohammad F., Munyaka, Peris M., Eissa, Nour, Metz-Boutigue, Marie-Hélène, Khafipour, Ehsan, Ghia, Jean Eric
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5239785/
https://www.ncbi.nlm.nih.gov/pubmed/28144234
http://dx.doi.org/10.3389/fmicb.2016.02151
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author Rabbi, Mohammad F.
Munyaka, Peris M.
Eissa, Nour
Metz-Boutigue, Marie-Hélène
Khafipour, Ehsan
Ghia, Jean Eric
author_facet Rabbi, Mohammad F.
Munyaka, Peris M.
Eissa, Nour
Metz-Boutigue, Marie-Hélène
Khafipour, Ehsan
Ghia, Jean Eric
author_sort Rabbi, Mohammad F.
collection PubMed
description The mammalian intestinal tract is heavily colonized with a dense, complex, and diversified microbial populations. In healthy individuals, an array of epithelial antimicrobial agents is secreted in the gut to aid intestinal homeostasis. Enterochromaffin cells (EC) in the intestinal epithelium are a major source of chromogranin A (CgA), which is a pro-hormone and can be cleaved into many bioactive peptides that include catestatin (CST). This study was carried out to evaluate the possible impact of CST on gut microbiota in vivo using a mouse model. The CST (Human CgA(352−372)) or normal saline was intrarectally administered in C57BL/6 male mice for 6 days and then sacrificed. Feces and colonic mucosa tissue samples were collected, DNA was extracted, the V4 region of bacterial 16S rRNA gene was amplified and subjected to MiSeq Illumina sequencing. The α-diversity was calculated using Chao 1 and β-diversity was determined using QIIME. Differences at the genus level were determined using partial least square discriminant analysis (PLS-DA). Phylogenetic investigation of communities by reconstruction of unobserved states (PICRUSt) was used to predict functional capacity of bacterial community. CST treatment did not modify bacterial richness in fecal and colonic mucosa-associated microbiota; however, treatment significantly modified bacterial community composition between the groups. Also, CST-treated mice had a significantly lower relative abundance of Firmicutes and higher abundance of Bacteroidetes, observed only in fecal samples. However, at lower phylogenetic levels, PLS-DA analysis revealed that some bacterial taxa were significantly associated with the CST-treated mice in both fecal and colonic mucosa samples. In addition, differences in predicted microbial functional pathways in both fecal and colonic mucosa samples were detected. The results support the hypothesis that CST treatment modulates gut microbiota composition under non-pathophysiological conditions, however, the result of this study needs to be further validated in a larger experiment. The data may open new avenues for the development of a potential new line of antimicrobial peptides and their use as therapeutic agents to treat several inflammatory conditions of the gastrointestinal tract, such as inflammatory bowel disease (IBD), inflammatory bowel syndrome (IBS), or other health conditions.
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spelling pubmed-52397852017-01-31 Human Catestatin Alters Gut Microbiota Composition in Mice Rabbi, Mohammad F. Munyaka, Peris M. Eissa, Nour Metz-Boutigue, Marie-Hélène Khafipour, Ehsan Ghia, Jean Eric Front Microbiol Microbiology The mammalian intestinal tract is heavily colonized with a dense, complex, and diversified microbial populations. In healthy individuals, an array of epithelial antimicrobial agents is secreted in the gut to aid intestinal homeostasis. Enterochromaffin cells (EC) in the intestinal epithelium are a major source of chromogranin A (CgA), which is a pro-hormone and can be cleaved into many bioactive peptides that include catestatin (CST). This study was carried out to evaluate the possible impact of CST on gut microbiota in vivo using a mouse model. The CST (Human CgA(352−372)) or normal saline was intrarectally administered in C57BL/6 male mice for 6 days and then sacrificed. Feces and colonic mucosa tissue samples were collected, DNA was extracted, the V4 region of bacterial 16S rRNA gene was amplified and subjected to MiSeq Illumina sequencing. The α-diversity was calculated using Chao 1 and β-diversity was determined using QIIME. Differences at the genus level were determined using partial least square discriminant analysis (PLS-DA). Phylogenetic investigation of communities by reconstruction of unobserved states (PICRUSt) was used to predict functional capacity of bacterial community. CST treatment did not modify bacterial richness in fecal and colonic mucosa-associated microbiota; however, treatment significantly modified bacterial community composition between the groups. Also, CST-treated mice had a significantly lower relative abundance of Firmicutes and higher abundance of Bacteroidetes, observed only in fecal samples. However, at lower phylogenetic levels, PLS-DA analysis revealed that some bacterial taxa were significantly associated with the CST-treated mice in both fecal and colonic mucosa samples. In addition, differences in predicted microbial functional pathways in both fecal and colonic mucosa samples were detected. The results support the hypothesis that CST treatment modulates gut microbiota composition under non-pathophysiological conditions, however, the result of this study needs to be further validated in a larger experiment. The data may open new avenues for the development of a potential new line of antimicrobial peptides and their use as therapeutic agents to treat several inflammatory conditions of the gastrointestinal tract, such as inflammatory bowel disease (IBD), inflammatory bowel syndrome (IBS), or other health conditions. Frontiers Media S.A. 2017-01-17 /pmc/articles/PMC5239785/ /pubmed/28144234 http://dx.doi.org/10.3389/fmicb.2016.02151 Text en Copyright © 2017 Rabbi, Munyaka, Eissa, Metz-Boutigue, Khafipour and Ghia. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Rabbi, Mohammad F.
Munyaka, Peris M.
Eissa, Nour
Metz-Boutigue, Marie-Hélène
Khafipour, Ehsan
Ghia, Jean Eric
Human Catestatin Alters Gut Microbiota Composition in Mice
title Human Catestatin Alters Gut Microbiota Composition in Mice
title_full Human Catestatin Alters Gut Microbiota Composition in Mice
title_fullStr Human Catestatin Alters Gut Microbiota Composition in Mice
title_full_unstemmed Human Catestatin Alters Gut Microbiota Composition in Mice
title_short Human Catestatin Alters Gut Microbiota Composition in Mice
title_sort human catestatin alters gut microbiota composition in mice
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5239785/
https://www.ncbi.nlm.nih.gov/pubmed/28144234
http://dx.doi.org/10.3389/fmicb.2016.02151
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