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Two Novel Sets of Genes Essential for Nicotine Degradation by Sphingomonas melonis TY
Nicotine is a type of environmental pollutant present in the tobacco waste that is generated during tobacco manufacturing. Sphingomonas melonis TY can utilize nicotine as a sole source of carbon, nitrogen and energy via a variant of the pyridine and pyrrolidine pathway (the VPP pathway). In this stu...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2017
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5239795/ https://www.ncbi.nlm.nih.gov/pubmed/28144232 http://dx.doi.org/10.3389/fmicb.2016.02060 |
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author | Wang, Haixia Xie, Cuixiao Zhu, Panpan Zhou, Ning-Yi Lu, Zhenmei |
author_facet | Wang, Haixia Xie, Cuixiao Zhu, Panpan Zhou, Ning-Yi Lu, Zhenmei |
author_sort | Wang, Haixia |
collection | PubMed |
description | Nicotine is a type of environmental pollutant present in the tobacco waste that is generated during tobacco manufacturing. Sphingomonas melonis TY can utilize nicotine as a sole source of carbon, nitrogen and energy via a variant of the pyridine and pyrrolidine pathway (the VPP pathway). In this study, we report the identification of two novel sets of genes, ndrA1A2A3, and ndrB1B2B3B4, which are crucial for nicotine degradation by strain TY. ndrA1A2A3 and ndrB1B2B3B4 exhibit similarity with both nicotine dehydrogenase ndh from Arthrobacter nicotinovorans and nicotine hydroxylase vppA from Ochrobactrum sp. SJY1. The transcriptional levels of ndrA1A2A3 and ndrB1B2B3B4 in strain TY were significantly upregulated in the presence of nicotine. Furthermore, ndrA1 or ndrB2 knockout resulted in a loss of the ability to degrade nicotine, whereas gene complementation restored the capacity of each mutant to utilize nicotine for growth. Biodegradation assays indicated that the mutant strains retained the ability to degrade the first intermediate in the pathway, 6-hydroxynicotine (6 HN). However, heterologous expression of ndrA1A2A3 and ndrB1B2B3B4 did not confer nicotine dehydrogenase activity to E. coli DH5α, Pseudomonas putida KT2440 or Sphingomonas aquatilis. These results provide information on the VPP pathway of nicotine degradation in S. melonis TY, and we conclude that these two sets of genes have essential functions in the conversion of nicotine to 6 HN in strain TY. |
format | Online Article Text |
id | pubmed-5239795 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-52397952017-01-31 Two Novel Sets of Genes Essential for Nicotine Degradation by Sphingomonas melonis TY Wang, Haixia Xie, Cuixiao Zhu, Panpan Zhou, Ning-Yi Lu, Zhenmei Front Microbiol Microbiology Nicotine is a type of environmental pollutant present in the tobacco waste that is generated during tobacco manufacturing. Sphingomonas melonis TY can utilize nicotine as a sole source of carbon, nitrogen and energy via a variant of the pyridine and pyrrolidine pathway (the VPP pathway). In this study, we report the identification of two novel sets of genes, ndrA1A2A3, and ndrB1B2B3B4, which are crucial for nicotine degradation by strain TY. ndrA1A2A3 and ndrB1B2B3B4 exhibit similarity with both nicotine dehydrogenase ndh from Arthrobacter nicotinovorans and nicotine hydroxylase vppA from Ochrobactrum sp. SJY1. The transcriptional levels of ndrA1A2A3 and ndrB1B2B3B4 in strain TY were significantly upregulated in the presence of nicotine. Furthermore, ndrA1 or ndrB2 knockout resulted in a loss of the ability to degrade nicotine, whereas gene complementation restored the capacity of each mutant to utilize nicotine for growth. Biodegradation assays indicated that the mutant strains retained the ability to degrade the first intermediate in the pathway, 6-hydroxynicotine (6 HN). However, heterologous expression of ndrA1A2A3 and ndrB1B2B3B4 did not confer nicotine dehydrogenase activity to E. coli DH5α, Pseudomonas putida KT2440 or Sphingomonas aquatilis. These results provide information on the VPP pathway of nicotine degradation in S. melonis TY, and we conclude that these two sets of genes have essential functions in the conversion of nicotine to 6 HN in strain TY. Frontiers Media S.A. 2017-01-17 /pmc/articles/PMC5239795/ /pubmed/28144232 http://dx.doi.org/10.3389/fmicb.2016.02060 Text en Copyright © 2017 Wang, Xie, Zhu, Zhou and Lu. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Wang, Haixia Xie, Cuixiao Zhu, Panpan Zhou, Ning-Yi Lu, Zhenmei Two Novel Sets of Genes Essential for Nicotine Degradation by Sphingomonas melonis TY |
title | Two Novel Sets of Genes Essential for Nicotine Degradation by Sphingomonas melonis TY |
title_full | Two Novel Sets of Genes Essential for Nicotine Degradation by Sphingomonas melonis TY |
title_fullStr | Two Novel Sets of Genes Essential for Nicotine Degradation by Sphingomonas melonis TY |
title_full_unstemmed | Two Novel Sets of Genes Essential for Nicotine Degradation by Sphingomonas melonis TY |
title_short | Two Novel Sets of Genes Essential for Nicotine Degradation by Sphingomonas melonis TY |
title_sort | two novel sets of genes essential for nicotine degradation by sphingomonas melonis ty |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5239795/ https://www.ncbi.nlm.nih.gov/pubmed/28144232 http://dx.doi.org/10.3389/fmicb.2016.02060 |
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