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Gene expression profiling at birth characterizing the preterm infant with early onset infection

ABSTRACT: Early onset infection (EOI) in preterm infants <32 weeks gestational age (GA) is associated with a high mortality rate and the development of severe acute and long-term complications. The pathophysiology of EOI is not fully understood and clinical and laboratory signs of early onset inf...

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Autores principales: Hilgendorff, Anne, Windhorst, Anita, Klein, Manuel, Tchatalbachev, Svetlin, Windemuth-Kieselbach, Christine, Kreuder, Joachim, Heckmann, Matthias, Gkatzoflia, Anna, Ehrhardt, Harald, Mysliwietz, Josef, Maier, Michael, Izar, Benjamin, Billion, Andre, Gortner, Ludwig, Chakraborty, Trinad, Hossain, Hamid
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5239802/
https://www.ncbi.nlm.nih.gov/pubmed/27576916
http://dx.doi.org/10.1007/s00109-016-1466-4
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author Hilgendorff, Anne
Windhorst, Anita
Klein, Manuel
Tchatalbachev, Svetlin
Windemuth-Kieselbach, Christine
Kreuder, Joachim
Heckmann, Matthias
Gkatzoflia, Anna
Ehrhardt, Harald
Mysliwietz, Josef
Maier, Michael
Izar, Benjamin
Billion, Andre
Gortner, Ludwig
Chakraborty, Trinad
Hossain, Hamid
author_facet Hilgendorff, Anne
Windhorst, Anita
Klein, Manuel
Tchatalbachev, Svetlin
Windemuth-Kieselbach, Christine
Kreuder, Joachim
Heckmann, Matthias
Gkatzoflia, Anna
Ehrhardt, Harald
Mysliwietz, Josef
Maier, Michael
Izar, Benjamin
Billion, Andre
Gortner, Ludwig
Chakraborty, Trinad
Hossain, Hamid
author_sort Hilgendorff, Anne
collection PubMed
description ABSTRACT: Early onset infection (EOI) in preterm infants <32 weeks gestational age (GA) is associated with a high mortality rate and the development of severe acute and long-term complications. The pathophysiology of EOI is not fully understood and clinical and laboratory signs of early onset infections in this patient cohort are often not conclusive. Thus, the aim of this study was to identify signatures characterizing preterm infants with EOI by using genome-wide gene expression (GWGE) analyses from umbilical arterial blood of preterm infants. This prospective cohort study was conducted in preterm infants <32 weeks GA. GWGE analyses using CodeLink human microarrays were performed from umbilical arterial blood of preterm infants with and without EOI. GWGE analyses revealed differential expression of 292 genes in preterm infants with EOI as compared to infants without EOI. Infants with EOI could be further differentiated into two subclasses and were distinguished by the magnitude of the expression of genes involved in both neutrophil and T cell activation. A hallmark activity for both subclasses of EOI was a common suppression of genes involved in natural killer (NK) cell function, which was independent from NK cell numbers. Significant results were recapitulated in an independent validation cohort. Gene expression profiling may enable early and more precise diagnosis of EOI in preterm infants. KEY MESSAGE: Gene expression (GE) profiling at birth characterizes preterm infants with EOI. GE analysis indicates dysregulation of NK cell activity. NK cell activity at birth may be a useful marker to improve early diagnosis of EOI. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s00109-016-1466-4) contains supplementary material, which is available to authorized users.
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spelling pubmed-52398022017-01-31 Gene expression profiling at birth characterizing the preterm infant with early onset infection Hilgendorff, Anne Windhorst, Anita Klein, Manuel Tchatalbachev, Svetlin Windemuth-Kieselbach, Christine Kreuder, Joachim Heckmann, Matthias Gkatzoflia, Anna Ehrhardt, Harald Mysliwietz, Josef Maier, Michael Izar, Benjamin Billion, Andre Gortner, Ludwig Chakraborty, Trinad Hossain, Hamid J Mol Med (Berl) Original Article ABSTRACT: Early onset infection (EOI) in preterm infants <32 weeks gestational age (GA) is associated with a high mortality rate and the development of severe acute and long-term complications. The pathophysiology of EOI is not fully understood and clinical and laboratory signs of early onset infections in this patient cohort are often not conclusive. Thus, the aim of this study was to identify signatures characterizing preterm infants with EOI by using genome-wide gene expression (GWGE) analyses from umbilical arterial blood of preterm infants. This prospective cohort study was conducted in preterm infants <32 weeks GA. GWGE analyses using CodeLink human microarrays were performed from umbilical arterial blood of preterm infants with and without EOI. GWGE analyses revealed differential expression of 292 genes in preterm infants with EOI as compared to infants without EOI. Infants with EOI could be further differentiated into two subclasses and were distinguished by the magnitude of the expression of genes involved in both neutrophil and T cell activation. A hallmark activity for both subclasses of EOI was a common suppression of genes involved in natural killer (NK) cell function, which was independent from NK cell numbers. Significant results were recapitulated in an independent validation cohort. Gene expression profiling may enable early and more precise diagnosis of EOI in preterm infants. KEY MESSAGE: Gene expression (GE) profiling at birth characterizes preterm infants with EOI. GE analysis indicates dysregulation of NK cell activity. NK cell activity at birth may be a useful marker to improve early diagnosis of EOI. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s00109-016-1466-4) contains supplementary material, which is available to authorized users. Springer Berlin Heidelberg 2016-08-30 2017 /pmc/articles/PMC5239802/ /pubmed/27576916 http://dx.doi.org/10.1007/s00109-016-1466-4 Text en © The Author(s) 2016 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Original Article
Hilgendorff, Anne
Windhorst, Anita
Klein, Manuel
Tchatalbachev, Svetlin
Windemuth-Kieselbach, Christine
Kreuder, Joachim
Heckmann, Matthias
Gkatzoflia, Anna
Ehrhardt, Harald
Mysliwietz, Josef
Maier, Michael
Izar, Benjamin
Billion, Andre
Gortner, Ludwig
Chakraborty, Trinad
Hossain, Hamid
Gene expression profiling at birth characterizing the preterm infant with early onset infection
title Gene expression profiling at birth characterizing the preterm infant with early onset infection
title_full Gene expression profiling at birth characterizing the preterm infant with early onset infection
title_fullStr Gene expression profiling at birth characterizing the preterm infant with early onset infection
title_full_unstemmed Gene expression profiling at birth characterizing the preterm infant with early onset infection
title_short Gene expression profiling at birth characterizing the preterm infant with early onset infection
title_sort gene expression profiling at birth characterizing the preterm infant with early onset infection
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5239802/
https://www.ncbi.nlm.nih.gov/pubmed/27576916
http://dx.doi.org/10.1007/s00109-016-1466-4
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