The different expression of TRPM7 and MagT1 impacts on the proliferation of colon carcinoma cells sensitive or resistant to doxorubicin

The processes leading to anticancer drug resistance are not completely unraveled. To get insights into the underlying mechanisms, we compared colon carcinoma cells sensitive to doxorubicin with their resistant counterpart. We found that resistant cells are growth retarded, and show staminal and ultr...

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Autores principales: Cazzaniga, Alessandra, Moscheni, Claudia, Trapani, Valentina, Wolf, Federica I., Farruggia, Giovanna, Sargenti, Azzurra, Iotti, Stefano, Maier, Jeanette A. M., Castiglioni, Sara
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2017
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Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5240092/
https://www.ncbi.nlm.nih.gov/pubmed/28094304
http://dx.doi.org/10.1038/srep40538
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author Cazzaniga, Alessandra
Moscheni, Claudia
Trapani, Valentina
Wolf, Federica I.
Farruggia, Giovanna
Sargenti, Azzurra
Iotti, Stefano
Maier, Jeanette A. M.
Castiglioni, Sara
author_facet Cazzaniga, Alessandra
Moscheni, Claudia
Trapani, Valentina
Wolf, Federica I.
Farruggia, Giovanna
Sargenti, Azzurra
Iotti, Stefano
Maier, Jeanette A. M.
Castiglioni, Sara
author_sort Cazzaniga, Alessandra
collection PubMed
description The processes leading to anticancer drug resistance are not completely unraveled. To get insights into the underlying mechanisms, we compared colon carcinoma cells sensitive to doxorubicin with their resistant counterpart. We found that resistant cells are growth retarded, and show staminal and ultrastructural features profoundly different from sensitive cells. The resistant phenotype is accompanied by the upregulation of the magnesium transporter MagT1 and the downregulation of the ion channel kinase TRPM7. We demonstrate that the different amounts of TRPM7 and MagT1 account for the different proliferation rate of sensitive and resistant colon carcinoma cells. It remains to be verified whether they are also involved in the control of other “staminal” traits.
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spelling pubmed-52400922017-01-23 The different expression of TRPM7 and MagT1 impacts on the proliferation of colon carcinoma cells sensitive or resistant to doxorubicin Cazzaniga, Alessandra Moscheni, Claudia Trapani, Valentina Wolf, Federica I. Farruggia, Giovanna Sargenti, Azzurra Iotti, Stefano Maier, Jeanette A. M. Castiglioni, Sara Sci Rep Article The processes leading to anticancer drug resistance are not completely unraveled. To get insights into the underlying mechanisms, we compared colon carcinoma cells sensitive to doxorubicin with their resistant counterpart. We found that resistant cells are growth retarded, and show staminal and ultrastructural features profoundly different from sensitive cells. The resistant phenotype is accompanied by the upregulation of the magnesium transporter MagT1 and the downregulation of the ion channel kinase TRPM7. We demonstrate that the different amounts of TRPM7 and MagT1 account for the different proliferation rate of sensitive and resistant colon carcinoma cells. It remains to be verified whether they are also involved in the control of other “staminal” traits. Nature Publishing Group 2017-01-17 /pmc/articles/PMC5240092/ /pubmed/28094304 http://dx.doi.org/10.1038/srep40538 Text en Copyright © 2017, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Cazzaniga, Alessandra
Moscheni, Claudia
Trapani, Valentina
Wolf, Federica I.
Farruggia, Giovanna
Sargenti, Azzurra
Iotti, Stefano
Maier, Jeanette A. M.
Castiglioni, Sara
The different expression of TRPM7 and MagT1 impacts on the proliferation of colon carcinoma cells sensitive or resistant to doxorubicin
title The different expression of TRPM7 and MagT1 impacts on the proliferation of colon carcinoma cells sensitive or resistant to doxorubicin
title_full The different expression of TRPM7 and MagT1 impacts on the proliferation of colon carcinoma cells sensitive or resistant to doxorubicin
title_fullStr The different expression of TRPM7 and MagT1 impacts on the proliferation of colon carcinoma cells sensitive or resistant to doxorubicin
title_full_unstemmed The different expression of TRPM7 and MagT1 impacts on the proliferation of colon carcinoma cells sensitive or resistant to doxorubicin
title_short The different expression of TRPM7 and MagT1 impacts on the proliferation of colon carcinoma cells sensitive or resistant to doxorubicin
title_sort different expression of trpm7 and magt1 impacts on the proliferation of colon carcinoma cells sensitive or resistant to doxorubicin
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5240092/
https://www.ncbi.nlm.nih.gov/pubmed/28094304
http://dx.doi.org/10.1038/srep40538
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