Gene design, fusion technology and TEV cleavage conditions influence the purification of oxidized disulphide-rich venom peptides in Escherichia coli

BACKGROUND: Animal venoms are large, complex libraries of bioactive, disulphide-rich peptides. These peptides, and their novel biological activities, are of increasing pharmacological and therapeutic importance. However, recombinant expression of venom peptides in Escherichia coli remains difficult...

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Detalles Bibliográficos
Autores principales: Sequeira, Ana Filipa, Turchetto, Jeremy, Saez, Natalie J., Peysson, Fanny, Ramond, Laurie, Duhoo, Yoan, Blémont, Marilyne, Fernandes, Vânia O., Gama, Luís T., Ferreira, Luís M. A., Guerreiro, Catarina I. P. I., Gilles, Nicolas, Darbon, Hervé, Fontes, Carlos M. G. A., Vincentelli, Renaud
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5240416/
https://www.ncbi.nlm.nih.gov/pubmed/28093085
http://dx.doi.org/10.1186/s12934-016-0618-0

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