Cargando…
Chromosomal organization of repetitive DNAs in Hordeum bogdanii and H. brevisubulatum (Poaceae)
Abstract. Molecular karyotypes of Hordeum bogdanii Wilensky, 1918 (2n = 14), and Hordeum brevisubulatum Link, 1844 ssp. brevisubulatum (2n = 28), were characterized by physical mapping of several repetitive sequences. A total of 18 repeats, including all possible di- or trinucleotide SSR (simple seq...
Autores principales: | , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Pensoft Publishers
2016
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5240503/ https://www.ncbi.nlm.nih.gov/pubmed/28123672 http://dx.doi.org/10.3897/CompCytogen.v10i4.9666 |
_version_ | 1782496083347243008 |
---|---|
author | Dou, Quanwen Liu, Ruijuan Yu, Feng |
author_facet | Dou, Quanwen Liu, Ruijuan Yu, Feng |
author_sort | Dou, Quanwen |
collection | PubMed |
description | Abstract. Molecular karyotypes of Hordeum bogdanii Wilensky, 1918 (2n = 14), and Hordeum brevisubulatum Link, 1844 ssp. brevisubulatum (2n = 28), were characterized by physical mapping of several repetitive sequences. A total of 18 repeats, including all possible di- or trinucleotide SSR (simple sequence repeat) motifs and satellite DNAs, such as pAs1, 5S rDNA, 45S rDNA, and pSc119.2, were used as probes for fluorescence in situ hybridization on root-tip metaphase chromosomes. Except for the SSR motifs AG, AT and GC, all the repeats we examined produced detectable hybridization signals on chromosomes of both species. A detailed molecular karyotype of the I genome of Hordeum bogdanii is described for the first time, and each repetitive sequence is physically mapped. A high degree of chromosome variation, including aneuploidy and structural changes, was observed in Hordeum brevisubulatum. Although the distribution of repeats in the chromosomes of Hordeum brevisubulatum is different from that of Hordeum bogdanii, similar patterns between the two species imply that the autopolyploid origin of Hordeum brevisubulatum is from a Hordeum species with an I genome. A comparison of the I genome and the other Hordeum genomes, H, Xa and Xu, shows that colocalization of motifs AAC, ACT and CAT and colocalization of motifs AAG and AGG are characteristic of the I genome. In addition, we discuss the evolutionary significance of repeats in the genome during genome differentiation. |
format | Online Article Text |
id | pubmed-5240503 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Pensoft Publishers |
record_format | MEDLINE/PubMed |
spelling | pubmed-52405032017-01-25 Chromosomal organization of repetitive DNAs in Hordeum bogdanii and H. brevisubulatum (Poaceae) Dou, Quanwen Liu, Ruijuan Yu, Feng Comp Cytogenet Research Article Abstract. Molecular karyotypes of Hordeum bogdanii Wilensky, 1918 (2n = 14), and Hordeum brevisubulatum Link, 1844 ssp. brevisubulatum (2n = 28), were characterized by physical mapping of several repetitive sequences. A total of 18 repeats, including all possible di- or trinucleotide SSR (simple sequence repeat) motifs and satellite DNAs, such as pAs1, 5S rDNA, 45S rDNA, and pSc119.2, were used as probes for fluorescence in situ hybridization on root-tip metaphase chromosomes. Except for the SSR motifs AG, AT and GC, all the repeats we examined produced detectable hybridization signals on chromosomes of both species. A detailed molecular karyotype of the I genome of Hordeum bogdanii is described for the first time, and each repetitive sequence is physically mapped. A high degree of chromosome variation, including aneuploidy and structural changes, was observed in Hordeum brevisubulatum. Although the distribution of repeats in the chromosomes of Hordeum brevisubulatum is different from that of Hordeum bogdanii, similar patterns between the two species imply that the autopolyploid origin of Hordeum brevisubulatum is from a Hordeum species with an I genome. A comparison of the I genome and the other Hordeum genomes, H, Xa and Xu, shows that colocalization of motifs AAC, ACT and CAT and colocalization of motifs AAG and AGG are characteristic of the I genome. In addition, we discuss the evolutionary significance of repeats in the genome during genome differentiation. Pensoft Publishers 2016-10-07 /pmc/articles/PMC5240503/ /pubmed/28123672 http://dx.doi.org/10.3897/CompCytogen.v10i4.9666 Text en Quanwen Dou, Ruijuan Liu, Feng Yu http://creativecommons.org/licenses/by/4.0 This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Dou, Quanwen Liu, Ruijuan Yu, Feng Chromosomal organization of repetitive DNAs in Hordeum bogdanii and H. brevisubulatum (Poaceae) |
title | Chromosomal organization of repetitive DNAs in Hordeum
bogdanii and H.
brevisubulatum (Poaceae) |
title_full | Chromosomal organization of repetitive DNAs in Hordeum
bogdanii and H.
brevisubulatum (Poaceae) |
title_fullStr | Chromosomal organization of repetitive DNAs in Hordeum
bogdanii and H.
brevisubulatum (Poaceae) |
title_full_unstemmed | Chromosomal organization of repetitive DNAs in Hordeum
bogdanii and H.
brevisubulatum (Poaceae) |
title_short | Chromosomal organization of repetitive DNAs in Hordeum
bogdanii and H.
brevisubulatum (Poaceae) |
title_sort | chromosomal organization of repetitive dnas in hordeum
bogdanii and h.
brevisubulatum (poaceae) |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5240503/ https://www.ncbi.nlm.nih.gov/pubmed/28123672 http://dx.doi.org/10.3897/CompCytogen.v10i4.9666 |
work_keys_str_mv | AT douquanwen chromosomalorganizationofrepetitivednasinhordeumbogdaniiandhbrevisubulatumpoaceae AT liuruijuan chromosomalorganizationofrepetitivednasinhordeumbogdaniiandhbrevisubulatumpoaceae AT yufeng chromosomalorganizationofrepetitivednasinhordeumbogdaniiandhbrevisubulatumpoaceae |