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Chromosome numbers and DNA content in some species of Mecardonia (Gratiolae, Plantaginaceae)

Abstract. Cytogenetic characterization and determination of DNA content by flow cytometry of five species of Mecardonia Ruiz et Pavon, 1798 (Gratiolae, Plantaginaceae) was performed. This is the first study of nuclear DNA content carried out in the genus. Mitotic analysis revealed a base chromosome...

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Autores principales: Sosa, María M., Angulo, María B., Greppi, Julián A., Bugallo, Verónica
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Pensoft Publishers 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5240523/
https://www.ncbi.nlm.nih.gov/pubmed/28123693
http://dx.doi.org/10.3897/CompCytogen.v10i4.10362
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author Sosa, María M.
Angulo, María B.
Greppi, Julián A.
Bugallo, Verónica
author_facet Sosa, María M.
Angulo, María B.
Greppi, Julián A.
Bugallo, Verónica
author_sort Sosa, María M.
collection PubMed
description Abstract. Cytogenetic characterization and determination of DNA content by flow cytometry of five species of Mecardonia Ruiz et Pavon, 1798 (Gratiolae, Plantaginaceae) was performed. This is the first study of nuclear DNA content carried out in the genus. Mitotic analysis revealed a base chromosome number x = 11 for all entities and different ploidy levels, ranging from diploid (2n = 2x = 22) to hexaploid (2n = 6x = 66). The results include the first report of the chromosome numbers for Mecardonia flagellaris (Chamisso & Schlechtendal, 1827) (2n = 22), Mecardonia grandiflora (Bentham) Pennell, 1946 (2n = 22), Mecardonia kamogawae Greppi & Hagiwara, 2011 (2n = 66), and Mecardonia sp. (2n = 44). The three ploidy levels here reported suggest that polyploidy is common in Mecardonia and appear to be an important factor in the evolution of this genus. The 2C- and 1Cx-values were also estimated in all the species. The 2C-values ranged from 1.91 to 5.29 pg. The 1Cx-values ranged from 0.88 to 1.03 pg. The general tendency indicated a decrease in the 1Cx-value with increasing ploidy level. The significance of the results is discussed in relation to taxonomy of the genus.
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spelling pubmed-52405232017-01-25 Chromosome numbers and DNA content in some species of Mecardonia (Gratiolae, Plantaginaceae) Sosa, María M. Angulo, María B. Greppi, Julián A. Bugallo, Verónica Comp Cytogenet Research Article Abstract. Cytogenetic characterization and determination of DNA content by flow cytometry of five species of Mecardonia Ruiz et Pavon, 1798 (Gratiolae, Plantaginaceae) was performed. This is the first study of nuclear DNA content carried out in the genus. Mitotic analysis revealed a base chromosome number x = 11 for all entities and different ploidy levels, ranging from diploid (2n = 2x = 22) to hexaploid (2n = 6x = 66). The results include the first report of the chromosome numbers for Mecardonia flagellaris (Chamisso & Schlechtendal, 1827) (2n = 22), Mecardonia grandiflora (Bentham) Pennell, 1946 (2n = 22), Mecardonia kamogawae Greppi & Hagiwara, 2011 (2n = 66), and Mecardonia sp. (2n = 44). The three ploidy levels here reported suggest that polyploidy is common in Mecardonia and appear to be an important factor in the evolution of this genus. The 2C- and 1Cx-values were also estimated in all the species. The 2C-values ranged from 1.91 to 5.29 pg. The 1Cx-values ranged from 0.88 to 1.03 pg. The general tendency indicated a decrease in the 1Cx-value with increasing ploidy level. The significance of the results is discussed in relation to taxonomy of the genus. Pensoft Publishers 2016-12-14 /pmc/articles/PMC5240523/ /pubmed/28123693 http://dx.doi.org/10.3897/CompCytogen.v10i4.10362 Text en María M. Sosa, María B. Angulo, Julián A. Greppi, Verónica Bugallo http://creativecommons.org/licenses/by/4.0 This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Sosa, María M.
Angulo, María B.
Greppi, Julián A.
Bugallo, Verónica
Chromosome numbers and DNA content in some species of Mecardonia (Gratiolae, Plantaginaceae)
title Chromosome numbers and DNA content in some species of Mecardonia (Gratiolae, Plantaginaceae)
title_full Chromosome numbers and DNA content in some species of Mecardonia (Gratiolae, Plantaginaceae)
title_fullStr Chromosome numbers and DNA content in some species of Mecardonia (Gratiolae, Plantaginaceae)
title_full_unstemmed Chromosome numbers and DNA content in some species of Mecardonia (Gratiolae, Plantaginaceae)
title_short Chromosome numbers and DNA content in some species of Mecardonia (Gratiolae, Plantaginaceae)
title_sort chromosome numbers and dna content in some species of mecardonia (gratiolae, plantaginaceae)
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5240523/
https://www.ncbi.nlm.nih.gov/pubmed/28123693
http://dx.doi.org/10.3897/CompCytogen.v10i4.10362
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