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Chromosome numbers and DNA content in some species of Mecardonia (Gratiolae, Plantaginaceae)
Abstract. Cytogenetic characterization and determination of DNA content by flow cytometry of five species of Mecardonia Ruiz et Pavon, 1798 (Gratiolae, Plantaginaceae) was performed. This is the first study of nuclear DNA content carried out in the genus. Mitotic analysis revealed a base chromosome...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Pensoft Publishers
2016
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5240523/ https://www.ncbi.nlm.nih.gov/pubmed/28123693 http://dx.doi.org/10.3897/CompCytogen.v10i4.10362 |
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author | Sosa, María M. Angulo, María B. Greppi, Julián A. Bugallo, Verónica |
author_facet | Sosa, María M. Angulo, María B. Greppi, Julián A. Bugallo, Verónica |
author_sort | Sosa, María M. |
collection | PubMed |
description | Abstract. Cytogenetic characterization and determination of DNA content by flow cytometry of five species of Mecardonia Ruiz et Pavon, 1798 (Gratiolae, Plantaginaceae) was performed. This is the first study of nuclear DNA content carried out in the genus. Mitotic analysis revealed a base chromosome number x = 11 for all entities and different ploidy levels, ranging from diploid (2n = 2x = 22) to hexaploid (2n = 6x = 66). The results include the first report of the chromosome numbers for Mecardonia flagellaris (Chamisso & Schlechtendal, 1827) (2n = 22), Mecardonia grandiflora (Bentham) Pennell, 1946 (2n = 22), Mecardonia kamogawae Greppi & Hagiwara, 2011 (2n = 66), and Mecardonia sp. (2n = 44). The three ploidy levels here reported suggest that polyploidy is common in Mecardonia and appear to be an important factor in the evolution of this genus. The 2C- and 1Cx-values were also estimated in all the species. The 2C-values ranged from 1.91 to 5.29 pg. The 1Cx-values ranged from 0.88 to 1.03 pg. The general tendency indicated a decrease in the 1Cx-value with increasing ploidy level. The significance of the results is discussed in relation to taxonomy of the genus. |
format | Online Article Text |
id | pubmed-5240523 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Pensoft Publishers |
record_format | MEDLINE/PubMed |
spelling | pubmed-52405232017-01-25 Chromosome numbers and DNA content in some species of Mecardonia (Gratiolae, Plantaginaceae) Sosa, María M. Angulo, María B. Greppi, Julián A. Bugallo, Verónica Comp Cytogenet Research Article Abstract. Cytogenetic characterization and determination of DNA content by flow cytometry of five species of Mecardonia Ruiz et Pavon, 1798 (Gratiolae, Plantaginaceae) was performed. This is the first study of nuclear DNA content carried out in the genus. Mitotic analysis revealed a base chromosome number x = 11 for all entities and different ploidy levels, ranging from diploid (2n = 2x = 22) to hexaploid (2n = 6x = 66). The results include the first report of the chromosome numbers for Mecardonia flagellaris (Chamisso & Schlechtendal, 1827) (2n = 22), Mecardonia grandiflora (Bentham) Pennell, 1946 (2n = 22), Mecardonia kamogawae Greppi & Hagiwara, 2011 (2n = 66), and Mecardonia sp. (2n = 44). The three ploidy levels here reported suggest that polyploidy is common in Mecardonia and appear to be an important factor in the evolution of this genus. The 2C- and 1Cx-values were also estimated in all the species. The 2C-values ranged from 1.91 to 5.29 pg. The 1Cx-values ranged from 0.88 to 1.03 pg. The general tendency indicated a decrease in the 1Cx-value with increasing ploidy level. The significance of the results is discussed in relation to taxonomy of the genus. Pensoft Publishers 2016-12-14 /pmc/articles/PMC5240523/ /pubmed/28123693 http://dx.doi.org/10.3897/CompCytogen.v10i4.10362 Text en María M. Sosa, María B. Angulo, Julián A. Greppi, Verónica Bugallo http://creativecommons.org/licenses/by/4.0 This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Sosa, María M. Angulo, María B. Greppi, Julián A. Bugallo, Verónica Chromosome numbers and DNA content in some species of Mecardonia (Gratiolae, Plantaginaceae) |
title | Chromosome numbers and DNA content in some species of Mecardonia (Gratiolae, Plantaginaceae) |
title_full | Chromosome numbers and DNA content in some species of Mecardonia (Gratiolae, Plantaginaceae) |
title_fullStr | Chromosome numbers and DNA content in some species of Mecardonia (Gratiolae, Plantaginaceae) |
title_full_unstemmed | Chromosome numbers and DNA content in some species of Mecardonia (Gratiolae, Plantaginaceae) |
title_short | Chromosome numbers and DNA content in some species of Mecardonia (Gratiolae, Plantaginaceae) |
title_sort | chromosome numbers and dna content in some species of mecardonia (gratiolae, plantaginaceae) |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5240523/ https://www.ncbi.nlm.nih.gov/pubmed/28123693 http://dx.doi.org/10.3897/CompCytogen.v10i4.10362 |
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