Cargando…

Butterfly eyespot organiser: in vivo imaging of the prospective focal cells in pupal wing tissues

Butterfly wing eyespot patterns are determined in pupal tissues by organisers located at the centre of the prospective eyespots. Nevertheless, organiser cells have not been examined cytochemically in vivo, partly due to technical difficulties. Here, we directly observed organiser cells in pupal fore...

Descripción completa

Detalles Bibliográficos
Autores principales: Iwasaki, Mayo, Ohno, Yoshikazu, Otaki, Joji M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5240560/
https://www.ncbi.nlm.nih.gov/pubmed/28094808
http://dx.doi.org/10.1038/srep40705
_version_ 1782496094498848768
author Iwasaki, Mayo
Ohno, Yoshikazu
Otaki, Joji M.
author_facet Iwasaki, Mayo
Ohno, Yoshikazu
Otaki, Joji M.
author_sort Iwasaki, Mayo
collection PubMed
description Butterfly wing eyespot patterns are determined in pupal tissues by organisers located at the centre of the prospective eyespots. Nevertheless, organiser cells have not been examined cytochemically in vivo, partly due to technical difficulties. Here, we directly observed organiser cells in pupal forewing epithelium via an in vivo confocal fluorescent imaging technique, using 1-h post-pupation pupae of the blue pansy butterfly, Junonia orithya. The prospective eyespot centre was indented from the plane of the ventral tissue surface. Three-dimensional reconstruction images revealed that the apical portion of “focal cells” at the bottom of the eyespot indentation contained many mitochondria. The mitochondrial portion was connected with a “cell body” containing a nucleus. Most focal cells had globular nuclei and were vertically elongated, but cells in the wing basal region had flattened nuclei and were tilted toward the distal direction. Epithelial cells in any wing region had cytoneme-like horizontal processes. From 1 h to 10 h post-pupation, nuclear volume increased, suggesting DNA synthesis during this period. Morphological differences among cells in different regions may suggest that organiser cells are developmentally ahead of cells in other regions and that position-dependent heterochronic development is a general mechanism for constructing colour patterns in butterfly wings.
format Online
Article
Text
id pubmed-5240560
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Nature Publishing Group
record_format MEDLINE/PubMed
spelling pubmed-52405602017-01-23 Butterfly eyespot organiser: in vivo imaging of the prospective focal cells in pupal wing tissues Iwasaki, Mayo Ohno, Yoshikazu Otaki, Joji M. Sci Rep Article Butterfly wing eyespot patterns are determined in pupal tissues by organisers located at the centre of the prospective eyespots. Nevertheless, organiser cells have not been examined cytochemically in vivo, partly due to technical difficulties. Here, we directly observed organiser cells in pupal forewing epithelium via an in vivo confocal fluorescent imaging technique, using 1-h post-pupation pupae of the blue pansy butterfly, Junonia orithya. The prospective eyespot centre was indented from the plane of the ventral tissue surface. Three-dimensional reconstruction images revealed that the apical portion of “focal cells” at the bottom of the eyespot indentation contained many mitochondria. The mitochondrial portion was connected with a “cell body” containing a nucleus. Most focal cells had globular nuclei and were vertically elongated, but cells in the wing basal region had flattened nuclei and were tilted toward the distal direction. Epithelial cells in any wing region had cytoneme-like horizontal processes. From 1 h to 10 h post-pupation, nuclear volume increased, suggesting DNA synthesis during this period. Morphological differences among cells in different regions may suggest that organiser cells are developmentally ahead of cells in other regions and that position-dependent heterochronic development is a general mechanism for constructing colour patterns in butterfly wings. Nature Publishing Group 2017-01-17 /pmc/articles/PMC5240560/ /pubmed/28094808 http://dx.doi.org/10.1038/srep40705 Text en Copyright © 2017, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Iwasaki, Mayo
Ohno, Yoshikazu
Otaki, Joji M.
Butterfly eyespot organiser: in vivo imaging of the prospective focal cells in pupal wing tissues
title Butterfly eyespot organiser: in vivo imaging of the prospective focal cells in pupal wing tissues
title_full Butterfly eyespot organiser: in vivo imaging of the prospective focal cells in pupal wing tissues
title_fullStr Butterfly eyespot organiser: in vivo imaging of the prospective focal cells in pupal wing tissues
title_full_unstemmed Butterfly eyespot organiser: in vivo imaging of the prospective focal cells in pupal wing tissues
title_short Butterfly eyespot organiser: in vivo imaging of the prospective focal cells in pupal wing tissues
title_sort butterfly eyespot organiser: in vivo imaging of the prospective focal cells in pupal wing tissues
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5240560/
https://www.ncbi.nlm.nih.gov/pubmed/28094808
http://dx.doi.org/10.1038/srep40705
work_keys_str_mv AT iwasakimayo butterflyeyespotorganiserinvivoimagingoftheprospectivefocalcellsinpupalwingtissues
AT ohnoyoshikazu butterflyeyespotorganiserinvivoimagingoftheprospectivefocalcellsinpupalwingtissues
AT otakijojim butterflyeyespotorganiserinvivoimagingoftheprospectivefocalcellsinpupalwingtissues