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Butterfly eyespot organiser: in vivo imaging of the prospective focal cells in pupal wing tissues
Butterfly wing eyespot patterns are determined in pupal tissues by organisers located at the centre of the prospective eyespots. Nevertheless, organiser cells have not been examined cytochemically in vivo, partly due to technical difficulties. Here, we directly observed organiser cells in pupal fore...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5240560/ https://www.ncbi.nlm.nih.gov/pubmed/28094808 http://dx.doi.org/10.1038/srep40705 |
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author | Iwasaki, Mayo Ohno, Yoshikazu Otaki, Joji M. |
author_facet | Iwasaki, Mayo Ohno, Yoshikazu Otaki, Joji M. |
author_sort | Iwasaki, Mayo |
collection | PubMed |
description | Butterfly wing eyespot patterns are determined in pupal tissues by organisers located at the centre of the prospective eyespots. Nevertheless, organiser cells have not been examined cytochemically in vivo, partly due to technical difficulties. Here, we directly observed organiser cells in pupal forewing epithelium via an in vivo confocal fluorescent imaging technique, using 1-h post-pupation pupae of the blue pansy butterfly, Junonia orithya. The prospective eyespot centre was indented from the plane of the ventral tissue surface. Three-dimensional reconstruction images revealed that the apical portion of “focal cells” at the bottom of the eyespot indentation contained many mitochondria. The mitochondrial portion was connected with a “cell body” containing a nucleus. Most focal cells had globular nuclei and were vertically elongated, but cells in the wing basal region had flattened nuclei and were tilted toward the distal direction. Epithelial cells in any wing region had cytoneme-like horizontal processes. From 1 h to 10 h post-pupation, nuclear volume increased, suggesting DNA synthesis during this period. Morphological differences among cells in different regions may suggest that organiser cells are developmentally ahead of cells in other regions and that position-dependent heterochronic development is a general mechanism for constructing colour patterns in butterfly wings. |
format | Online Article Text |
id | pubmed-5240560 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-52405602017-01-23 Butterfly eyespot organiser: in vivo imaging of the prospective focal cells in pupal wing tissues Iwasaki, Mayo Ohno, Yoshikazu Otaki, Joji M. Sci Rep Article Butterfly wing eyespot patterns are determined in pupal tissues by organisers located at the centre of the prospective eyespots. Nevertheless, organiser cells have not been examined cytochemically in vivo, partly due to technical difficulties. Here, we directly observed organiser cells in pupal forewing epithelium via an in vivo confocal fluorescent imaging technique, using 1-h post-pupation pupae of the blue pansy butterfly, Junonia orithya. The prospective eyespot centre was indented from the plane of the ventral tissue surface. Three-dimensional reconstruction images revealed that the apical portion of “focal cells” at the bottom of the eyespot indentation contained many mitochondria. The mitochondrial portion was connected with a “cell body” containing a nucleus. Most focal cells had globular nuclei and were vertically elongated, but cells in the wing basal region had flattened nuclei and were tilted toward the distal direction. Epithelial cells in any wing region had cytoneme-like horizontal processes. From 1 h to 10 h post-pupation, nuclear volume increased, suggesting DNA synthesis during this period. Morphological differences among cells in different regions may suggest that organiser cells are developmentally ahead of cells in other regions and that position-dependent heterochronic development is a general mechanism for constructing colour patterns in butterfly wings. Nature Publishing Group 2017-01-17 /pmc/articles/PMC5240560/ /pubmed/28094808 http://dx.doi.org/10.1038/srep40705 Text en Copyright © 2017, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Iwasaki, Mayo Ohno, Yoshikazu Otaki, Joji M. Butterfly eyespot organiser: in vivo imaging of the prospective focal cells in pupal wing tissues |
title | Butterfly eyespot organiser: in vivo imaging of the prospective focal cells in pupal wing tissues |
title_full | Butterfly eyespot organiser: in vivo imaging of the prospective focal cells in pupal wing tissues |
title_fullStr | Butterfly eyespot organiser: in vivo imaging of the prospective focal cells in pupal wing tissues |
title_full_unstemmed | Butterfly eyespot organiser: in vivo imaging of the prospective focal cells in pupal wing tissues |
title_short | Butterfly eyespot organiser: in vivo imaging of the prospective focal cells in pupal wing tissues |
title_sort | butterfly eyespot organiser: in vivo imaging of the prospective focal cells in pupal wing tissues |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5240560/ https://www.ncbi.nlm.nih.gov/pubmed/28094808 http://dx.doi.org/10.1038/srep40705 |
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