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Expression and bioactivity of human α-fetoprotein in a Bac-to-Bac system

α-fetoprotein (AFP) is an early serum growth factor in foetal embryonic development and hepatic oncogenesis. A growing number of investigations of AFP as a tumour-specific biomarker have concluded that AFP is an important target for cancer treatment. AFP also plays an immunomodulatory role in the tr...

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Autores principales: Lin, Bo, Liu, Kun, Wang, Wenting, Li, Wei, Dong, Xu, Chen, Yi, Lu, Yan, Guo, Junli, Zhu, Mingyue, Li, Mengsen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Portland Press Ltd. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5240590/
https://www.ncbi.nlm.nih.gov/pubmed/27913752
http://dx.doi.org/10.1042/BSR20160161
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author Lin, Bo
Liu, Kun
Wang, Wenting
Li, Wei
Dong, Xu
Chen, Yi
Lu, Yan
Guo, Junli
Zhu, Mingyue
Li, Mengsen
author_facet Lin, Bo
Liu, Kun
Wang, Wenting
Li, Wei
Dong, Xu
Chen, Yi
Lu, Yan
Guo, Junli
Zhu, Mingyue
Li, Mengsen
author_sort Lin, Bo
collection PubMed
description α-fetoprotein (AFP) is an early serum growth factor in foetal embryonic development and hepatic oncogenesis. A growing number of investigations of AFP as a tumour-specific biomarker have concluded that AFP is an important target for cancer treatment. AFP also plays an immunomodulatory role in the treatment of several autoimmune diseases, such as rheumatoid arthritis, multiple sclerosis, myasthenia gravis and thyroiditis. In an effort to support biochemical screening and drug design and discovery, we attempted to express and purify human AFP in a Bac-to-Bac system. Two key factors affecting the expression of recombinant human AFP (R-AFP), namely the infectious baculovirus inoculum volume and the culturing time post-infection, were optimized to maximize the yield. We achieved a high yield of approximately 1.5 mg/l of harvested medium with a 72–96 h incubation period after infection and an inoculum volume ratio of 1:100. We also assessed the role of R-AFP in the proliferation of the human liver cancer cell line Bel 7402, and the results indicated that R-AFP promoted the growth of hepatoma cells. We concluded that this method can produce high yields of R-AFP, which can be used for studies related to AFP.
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spelling pubmed-52405902017-02-28 Expression and bioactivity of human α-fetoprotein in a Bac-to-Bac system Lin, Bo Liu, Kun Wang, Wenting Li, Wei Dong, Xu Chen, Yi Lu, Yan Guo, Junli Zhu, Mingyue Li, Mengsen Biosci Rep Research Articles α-fetoprotein (AFP) is an early serum growth factor in foetal embryonic development and hepatic oncogenesis. A growing number of investigations of AFP as a tumour-specific biomarker have concluded that AFP is an important target for cancer treatment. AFP also plays an immunomodulatory role in the treatment of several autoimmune diseases, such as rheumatoid arthritis, multiple sclerosis, myasthenia gravis and thyroiditis. In an effort to support biochemical screening and drug design and discovery, we attempted to express and purify human AFP in a Bac-to-Bac system. Two key factors affecting the expression of recombinant human AFP (R-AFP), namely the infectious baculovirus inoculum volume and the culturing time post-infection, were optimized to maximize the yield. We achieved a high yield of approximately 1.5 mg/l of harvested medium with a 72–96 h incubation period after infection and an inoculum volume ratio of 1:100. We also assessed the role of R-AFP in the proliferation of the human liver cancer cell line Bel 7402, and the results indicated that R-AFP promoted the growth of hepatoma cells. We concluded that this method can produce high yields of R-AFP, which can be used for studies related to AFP. Portland Press Ltd. 2017-01-17 /pmc/articles/PMC5240590/ /pubmed/27913752 http://dx.doi.org/10.1042/BSR20160161 Text en © 2017 The Author(s) http://creativecommons.org/licenses/by/4.0/This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY) (http://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research Articles
Lin, Bo
Liu, Kun
Wang, Wenting
Li, Wei
Dong, Xu
Chen, Yi
Lu, Yan
Guo, Junli
Zhu, Mingyue
Li, Mengsen
Expression and bioactivity of human α-fetoprotein in a Bac-to-Bac system
title Expression and bioactivity of human α-fetoprotein in a Bac-to-Bac system
title_full Expression and bioactivity of human α-fetoprotein in a Bac-to-Bac system
title_fullStr Expression and bioactivity of human α-fetoprotein in a Bac-to-Bac system
title_full_unstemmed Expression and bioactivity of human α-fetoprotein in a Bac-to-Bac system
title_short Expression and bioactivity of human α-fetoprotein in a Bac-to-Bac system
title_sort expression and bioactivity of human α-fetoprotein in a bac-to-bac system
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5240590/
https://www.ncbi.nlm.nih.gov/pubmed/27913752
http://dx.doi.org/10.1042/BSR20160161
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