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Expression and bioactivity of human α-fetoprotein in a Bac-to-Bac system
α-fetoprotein (AFP) is an early serum growth factor in foetal embryonic development and hepatic oncogenesis. A growing number of investigations of AFP as a tumour-specific biomarker have concluded that AFP is an important target for cancer treatment. AFP also plays an immunomodulatory role in the tr...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Portland Press Ltd.
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5240590/ https://www.ncbi.nlm.nih.gov/pubmed/27913752 http://dx.doi.org/10.1042/BSR20160161 |
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author | Lin, Bo Liu, Kun Wang, Wenting Li, Wei Dong, Xu Chen, Yi Lu, Yan Guo, Junli Zhu, Mingyue Li, Mengsen |
author_facet | Lin, Bo Liu, Kun Wang, Wenting Li, Wei Dong, Xu Chen, Yi Lu, Yan Guo, Junli Zhu, Mingyue Li, Mengsen |
author_sort | Lin, Bo |
collection | PubMed |
description | α-fetoprotein (AFP) is an early serum growth factor in foetal embryonic development and hepatic oncogenesis. A growing number of investigations of AFP as a tumour-specific biomarker have concluded that AFP is an important target for cancer treatment. AFP also plays an immunomodulatory role in the treatment of several autoimmune diseases, such as rheumatoid arthritis, multiple sclerosis, myasthenia gravis and thyroiditis. In an effort to support biochemical screening and drug design and discovery, we attempted to express and purify human AFP in a Bac-to-Bac system. Two key factors affecting the expression of recombinant human AFP (R-AFP), namely the infectious baculovirus inoculum volume and the culturing time post-infection, were optimized to maximize the yield. We achieved a high yield of approximately 1.5 mg/l of harvested medium with a 72–96 h incubation period after infection and an inoculum volume ratio of 1:100. We also assessed the role of R-AFP in the proliferation of the human liver cancer cell line Bel 7402, and the results indicated that R-AFP promoted the growth of hepatoma cells. We concluded that this method can produce high yields of R-AFP, which can be used for studies related to AFP. |
format | Online Article Text |
id | pubmed-5240590 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Portland Press Ltd. |
record_format | MEDLINE/PubMed |
spelling | pubmed-52405902017-02-28 Expression and bioactivity of human α-fetoprotein in a Bac-to-Bac system Lin, Bo Liu, Kun Wang, Wenting Li, Wei Dong, Xu Chen, Yi Lu, Yan Guo, Junli Zhu, Mingyue Li, Mengsen Biosci Rep Research Articles α-fetoprotein (AFP) is an early serum growth factor in foetal embryonic development and hepatic oncogenesis. A growing number of investigations of AFP as a tumour-specific biomarker have concluded that AFP is an important target for cancer treatment. AFP also plays an immunomodulatory role in the treatment of several autoimmune diseases, such as rheumatoid arthritis, multiple sclerosis, myasthenia gravis and thyroiditis. In an effort to support biochemical screening and drug design and discovery, we attempted to express and purify human AFP in a Bac-to-Bac system. Two key factors affecting the expression of recombinant human AFP (R-AFP), namely the infectious baculovirus inoculum volume and the culturing time post-infection, were optimized to maximize the yield. We achieved a high yield of approximately 1.5 mg/l of harvested medium with a 72–96 h incubation period after infection and an inoculum volume ratio of 1:100. We also assessed the role of R-AFP in the proliferation of the human liver cancer cell line Bel 7402, and the results indicated that R-AFP promoted the growth of hepatoma cells. We concluded that this method can produce high yields of R-AFP, which can be used for studies related to AFP. Portland Press Ltd. 2017-01-17 /pmc/articles/PMC5240590/ /pubmed/27913752 http://dx.doi.org/10.1042/BSR20160161 Text en © 2017 The Author(s) http://creativecommons.org/licenses/by/4.0/This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY) (http://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Research Articles Lin, Bo Liu, Kun Wang, Wenting Li, Wei Dong, Xu Chen, Yi Lu, Yan Guo, Junli Zhu, Mingyue Li, Mengsen Expression and bioactivity of human α-fetoprotein in a Bac-to-Bac system |
title | Expression and bioactivity of human α-fetoprotein in a Bac-to-Bac system |
title_full | Expression and bioactivity of human α-fetoprotein in a Bac-to-Bac system |
title_fullStr | Expression and bioactivity of human α-fetoprotein in a Bac-to-Bac system |
title_full_unstemmed | Expression and bioactivity of human α-fetoprotein in a Bac-to-Bac system |
title_short | Expression and bioactivity of human α-fetoprotein in a Bac-to-Bac system |
title_sort | expression and bioactivity of human α-fetoprotein in a bac-to-bac system |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5240590/ https://www.ncbi.nlm.nih.gov/pubmed/27913752 http://dx.doi.org/10.1042/BSR20160161 |
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