Cargando…

Field Evaluation of a High Throughput Loop Mediated Isothermal Amplification Test for the Detection of Asymptomatic Plasmodium Infections in Zanzibar

BACKGROUND: New field applicable diagnostic tools are needed for highly sensitive detection of residual malaria infections in pre-elimination settings. Field performance of a high throughput DNA extraction system for loop mediated isothermal amplification (HTP-LAMP) was therefore evaluated for detec...

Descripción completa

Detalles Bibliográficos
Autores principales: Aydin-Schmidt, Berit, Morris, Ulrika, Ding, Xavier C., Jovel, Irina, Msellem, Mwinyi I., Bergman, Daniel, Islam, Atiqul, Ali, Abdullah S., Polley, Spencer, Gonzalez, Iveth J., Mårtensson, Andreas, Björkman, Anders
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5240913/
https://www.ncbi.nlm.nih.gov/pubmed/28095434
http://dx.doi.org/10.1371/journal.pone.0169037
_version_ 1782496120882069504
author Aydin-Schmidt, Berit
Morris, Ulrika
Ding, Xavier C.
Jovel, Irina
Msellem, Mwinyi I.
Bergman, Daniel
Islam, Atiqul
Ali, Abdullah S.
Polley, Spencer
Gonzalez, Iveth J.
Mårtensson, Andreas
Björkman, Anders
author_facet Aydin-Schmidt, Berit
Morris, Ulrika
Ding, Xavier C.
Jovel, Irina
Msellem, Mwinyi I.
Bergman, Daniel
Islam, Atiqul
Ali, Abdullah S.
Polley, Spencer
Gonzalez, Iveth J.
Mårtensson, Andreas
Björkman, Anders
author_sort Aydin-Schmidt, Berit
collection PubMed
description BACKGROUND: New field applicable diagnostic tools are needed for highly sensitive detection of residual malaria infections in pre-elimination settings. Field performance of a high throughput DNA extraction system for loop mediated isothermal amplification (HTP-LAMP) was therefore evaluated for detecting malaria parasites among asymptomatic individuals in Zanzibar. METHODS: HTP-LAMP performance was evaluated against real-time PCR on 3008 paired blood samples collected on filter papers in a community-based survey in 2015. RESULTS: The PCR and HTP-LAMP determined malaria prevalences were 1.6% (95%CI 1.3–2.4) and 0.7% (95%CI 0.4–1.1), respectively. The sensitivity of HTP-LAMP compared to PCR was 40.8% (CI95% 27.0–55.8) and the specificity was 99.9% (CI95% 99.8–100). For the PCR positive samples, there was no statistically significant difference between the geometric mean parasite densities among the HTP-LAMP positive (2.5 p/μL, range 0.2–770) and HTP-LAMP negative (1.4 p/μL, range 0.1–7) samples (p = 0.088). Two lab technicians analysed up to 282 samples per day and the HTP-LAMP method was experienced as user friendly. CONCLUSIONS: Although field applicable, this high throughput format of LAMP as used here was not sensitive enough to be recommended for detection of asymptomatic low-density infections in areas like Zanzibar, approaching malaria elimination.
format Online
Article
Text
id pubmed-5240913
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-52409132017-02-06 Field Evaluation of a High Throughput Loop Mediated Isothermal Amplification Test for the Detection of Asymptomatic Plasmodium Infections in Zanzibar Aydin-Schmidt, Berit Morris, Ulrika Ding, Xavier C. Jovel, Irina Msellem, Mwinyi I. Bergman, Daniel Islam, Atiqul Ali, Abdullah S. Polley, Spencer Gonzalez, Iveth J. Mårtensson, Andreas Björkman, Anders PLoS One Research Article BACKGROUND: New field applicable diagnostic tools are needed for highly sensitive detection of residual malaria infections in pre-elimination settings. Field performance of a high throughput DNA extraction system for loop mediated isothermal amplification (HTP-LAMP) was therefore evaluated for detecting malaria parasites among asymptomatic individuals in Zanzibar. METHODS: HTP-LAMP performance was evaluated against real-time PCR on 3008 paired blood samples collected on filter papers in a community-based survey in 2015. RESULTS: The PCR and HTP-LAMP determined malaria prevalences were 1.6% (95%CI 1.3–2.4) and 0.7% (95%CI 0.4–1.1), respectively. The sensitivity of HTP-LAMP compared to PCR was 40.8% (CI95% 27.0–55.8) and the specificity was 99.9% (CI95% 99.8–100). For the PCR positive samples, there was no statistically significant difference between the geometric mean parasite densities among the HTP-LAMP positive (2.5 p/μL, range 0.2–770) and HTP-LAMP negative (1.4 p/μL, range 0.1–7) samples (p = 0.088). Two lab technicians analysed up to 282 samples per day and the HTP-LAMP method was experienced as user friendly. CONCLUSIONS: Although field applicable, this high throughput format of LAMP as used here was not sensitive enough to be recommended for detection of asymptomatic low-density infections in areas like Zanzibar, approaching malaria elimination. Public Library of Science 2017-01-17 /pmc/articles/PMC5240913/ /pubmed/28095434 http://dx.doi.org/10.1371/journal.pone.0169037 Text en © 2017 Aydin-Schmidt et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Aydin-Schmidt, Berit
Morris, Ulrika
Ding, Xavier C.
Jovel, Irina
Msellem, Mwinyi I.
Bergman, Daniel
Islam, Atiqul
Ali, Abdullah S.
Polley, Spencer
Gonzalez, Iveth J.
Mårtensson, Andreas
Björkman, Anders
Field Evaluation of a High Throughput Loop Mediated Isothermal Amplification Test for the Detection of Asymptomatic Plasmodium Infections in Zanzibar
title Field Evaluation of a High Throughput Loop Mediated Isothermal Amplification Test for the Detection of Asymptomatic Plasmodium Infections in Zanzibar
title_full Field Evaluation of a High Throughput Loop Mediated Isothermal Amplification Test for the Detection of Asymptomatic Plasmodium Infections in Zanzibar
title_fullStr Field Evaluation of a High Throughput Loop Mediated Isothermal Amplification Test for the Detection of Asymptomatic Plasmodium Infections in Zanzibar
title_full_unstemmed Field Evaluation of a High Throughput Loop Mediated Isothermal Amplification Test for the Detection of Asymptomatic Plasmodium Infections in Zanzibar
title_short Field Evaluation of a High Throughput Loop Mediated Isothermal Amplification Test for the Detection of Asymptomatic Plasmodium Infections in Zanzibar
title_sort field evaluation of a high throughput loop mediated isothermal amplification test for the detection of asymptomatic plasmodium infections in zanzibar
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5240913/
https://www.ncbi.nlm.nih.gov/pubmed/28095434
http://dx.doi.org/10.1371/journal.pone.0169037
work_keys_str_mv AT aydinschmidtberit fieldevaluationofahighthroughputloopmediatedisothermalamplificationtestforthedetectionofasymptomaticplasmodiuminfectionsinzanzibar
AT morrisulrika fieldevaluationofahighthroughputloopmediatedisothermalamplificationtestforthedetectionofasymptomaticplasmodiuminfectionsinzanzibar
AT dingxavierc fieldevaluationofahighthroughputloopmediatedisothermalamplificationtestforthedetectionofasymptomaticplasmodiuminfectionsinzanzibar
AT jovelirina fieldevaluationofahighthroughputloopmediatedisothermalamplificationtestforthedetectionofasymptomaticplasmodiuminfectionsinzanzibar
AT msellemmwinyii fieldevaluationofahighthroughputloopmediatedisothermalamplificationtestforthedetectionofasymptomaticplasmodiuminfectionsinzanzibar
AT bergmandaniel fieldevaluationofahighthroughputloopmediatedisothermalamplificationtestforthedetectionofasymptomaticplasmodiuminfectionsinzanzibar
AT islamatiqul fieldevaluationofahighthroughputloopmediatedisothermalamplificationtestforthedetectionofasymptomaticplasmodiuminfectionsinzanzibar
AT aliabdullahs fieldevaluationofahighthroughputloopmediatedisothermalamplificationtestforthedetectionofasymptomaticplasmodiuminfectionsinzanzibar
AT polleyspencer fieldevaluationofahighthroughputloopmediatedisothermalamplificationtestforthedetectionofasymptomaticplasmodiuminfectionsinzanzibar
AT gonzalezivethj fieldevaluationofahighthroughputloopmediatedisothermalamplificationtestforthedetectionofasymptomaticplasmodiuminfectionsinzanzibar
AT martenssonandreas fieldevaluationofahighthroughputloopmediatedisothermalamplificationtestforthedetectionofasymptomaticplasmodiuminfectionsinzanzibar
AT bjorkmananders fieldevaluationofahighthroughputloopmediatedisothermalamplificationtestforthedetectionofasymptomaticplasmodiuminfectionsinzanzibar