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Field Evaluation of a High Throughput Loop Mediated Isothermal Amplification Test for the Detection of Asymptomatic Plasmodium Infections in Zanzibar
BACKGROUND: New field applicable diagnostic tools are needed for highly sensitive detection of residual malaria infections in pre-elimination settings. Field performance of a high throughput DNA extraction system for loop mediated isothermal amplification (HTP-LAMP) was therefore evaluated for detec...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5240913/ https://www.ncbi.nlm.nih.gov/pubmed/28095434 http://dx.doi.org/10.1371/journal.pone.0169037 |
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author | Aydin-Schmidt, Berit Morris, Ulrika Ding, Xavier C. Jovel, Irina Msellem, Mwinyi I. Bergman, Daniel Islam, Atiqul Ali, Abdullah S. Polley, Spencer Gonzalez, Iveth J. Mårtensson, Andreas Björkman, Anders |
author_facet | Aydin-Schmidt, Berit Morris, Ulrika Ding, Xavier C. Jovel, Irina Msellem, Mwinyi I. Bergman, Daniel Islam, Atiqul Ali, Abdullah S. Polley, Spencer Gonzalez, Iveth J. Mårtensson, Andreas Björkman, Anders |
author_sort | Aydin-Schmidt, Berit |
collection | PubMed |
description | BACKGROUND: New field applicable diagnostic tools are needed for highly sensitive detection of residual malaria infections in pre-elimination settings. Field performance of a high throughput DNA extraction system for loop mediated isothermal amplification (HTP-LAMP) was therefore evaluated for detecting malaria parasites among asymptomatic individuals in Zanzibar. METHODS: HTP-LAMP performance was evaluated against real-time PCR on 3008 paired blood samples collected on filter papers in a community-based survey in 2015. RESULTS: The PCR and HTP-LAMP determined malaria prevalences were 1.6% (95%CI 1.3–2.4) and 0.7% (95%CI 0.4–1.1), respectively. The sensitivity of HTP-LAMP compared to PCR was 40.8% (CI95% 27.0–55.8) and the specificity was 99.9% (CI95% 99.8–100). For the PCR positive samples, there was no statistically significant difference between the geometric mean parasite densities among the HTP-LAMP positive (2.5 p/μL, range 0.2–770) and HTP-LAMP negative (1.4 p/μL, range 0.1–7) samples (p = 0.088). Two lab technicians analysed up to 282 samples per day and the HTP-LAMP method was experienced as user friendly. CONCLUSIONS: Although field applicable, this high throughput format of LAMP as used here was not sensitive enough to be recommended for detection of asymptomatic low-density infections in areas like Zanzibar, approaching malaria elimination. |
format | Online Article Text |
id | pubmed-5240913 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-52409132017-02-06 Field Evaluation of a High Throughput Loop Mediated Isothermal Amplification Test for the Detection of Asymptomatic Plasmodium Infections in Zanzibar Aydin-Schmidt, Berit Morris, Ulrika Ding, Xavier C. Jovel, Irina Msellem, Mwinyi I. Bergman, Daniel Islam, Atiqul Ali, Abdullah S. Polley, Spencer Gonzalez, Iveth J. Mårtensson, Andreas Björkman, Anders PLoS One Research Article BACKGROUND: New field applicable diagnostic tools are needed for highly sensitive detection of residual malaria infections in pre-elimination settings. Field performance of a high throughput DNA extraction system for loop mediated isothermal amplification (HTP-LAMP) was therefore evaluated for detecting malaria parasites among asymptomatic individuals in Zanzibar. METHODS: HTP-LAMP performance was evaluated against real-time PCR on 3008 paired blood samples collected on filter papers in a community-based survey in 2015. RESULTS: The PCR and HTP-LAMP determined malaria prevalences were 1.6% (95%CI 1.3–2.4) and 0.7% (95%CI 0.4–1.1), respectively. The sensitivity of HTP-LAMP compared to PCR was 40.8% (CI95% 27.0–55.8) and the specificity was 99.9% (CI95% 99.8–100). For the PCR positive samples, there was no statistically significant difference between the geometric mean parasite densities among the HTP-LAMP positive (2.5 p/μL, range 0.2–770) and HTP-LAMP negative (1.4 p/μL, range 0.1–7) samples (p = 0.088). Two lab technicians analysed up to 282 samples per day and the HTP-LAMP method was experienced as user friendly. CONCLUSIONS: Although field applicable, this high throughput format of LAMP as used here was not sensitive enough to be recommended for detection of asymptomatic low-density infections in areas like Zanzibar, approaching malaria elimination. Public Library of Science 2017-01-17 /pmc/articles/PMC5240913/ /pubmed/28095434 http://dx.doi.org/10.1371/journal.pone.0169037 Text en © 2017 Aydin-Schmidt et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Aydin-Schmidt, Berit Morris, Ulrika Ding, Xavier C. Jovel, Irina Msellem, Mwinyi I. Bergman, Daniel Islam, Atiqul Ali, Abdullah S. Polley, Spencer Gonzalez, Iveth J. Mårtensson, Andreas Björkman, Anders Field Evaluation of a High Throughput Loop Mediated Isothermal Amplification Test for the Detection of Asymptomatic Plasmodium Infections in Zanzibar |
title | Field Evaluation of a High Throughput Loop Mediated Isothermal Amplification Test for the Detection of Asymptomatic Plasmodium Infections in Zanzibar |
title_full | Field Evaluation of a High Throughput Loop Mediated Isothermal Amplification Test for the Detection of Asymptomatic Plasmodium Infections in Zanzibar |
title_fullStr | Field Evaluation of a High Throughput Loop Mediated Isothermal Amplification Test for the Detection of Asymptomatic Plasmodium Infections in Zanzibar |
title_full_unstemmed | Field Evaluation of a High Throughput Loop Mediated Isothermal Amplification Test for the Detection of Asymptomatic Plasmodium Infections in Zanzibar |
title_short | Field Evaluation of a High Throughput Loop Mediated Isothermal Amplification Test for the Detection of Asymptomatic Plasmodium Infections in Zanzibar |
title_sort | field evaluation of a high throughput loop mediated isothermal amplification test for the detection of asymptomatic plasmodium infections in zanzibar |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5240913/ https://www.ncbi.nlm.nih.gov/pubmed/28095434 http://dx.doi.org/10.1371/journal.pone.0169037 |
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