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Experimental Diabetes Alters the Morphology and Nano-Structure of the Achilles Tendon

Although of several studies that associate chronic hyperglycemia with tendinopathy, the connection between morphometric changes as witnessed by magnetic resonance (MR) images, nanostructural changes, and inflammatory markers have not yet been fully established. Therefore, the present study has as a...

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Autores principales: de Oliveira, Rodrigo Ribeiro, Medina de Mattos, Rômulo, Magalhães Rebelo, Luciana, Guimarães Meireles Ferreira, Fernanda, Tovar-Moll, Fernanda, Eurico Nasciutti, Luiz, de Castro Brito, Gerly Anne
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5240962/
https://www.ncbi.nlm.nih.gov/pubmed/28095484
http://dx.doi.org/10.1371/journal.pone.0169513
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author de Oliveira, Rodrigo Ribeiro
Medina de Mattos, Rômulo
Magalhães Rebelo, Luciana
Guimarães Meireles Ferreira, Fernanda
Tovar-Moll, Fernanda
Eurico Nasciutti, Luiz
de Castro Brito, Gerly Anne
author_facet de Oliveira, Rodrigo Ribeiro
Medina de Mattos, Rômulo
Magalhães Rebelo, Luciana
Guimarães Meireles Ferreira, Fernanda
Tovar-Moll, Fernanda
Eurico Nasciutti, Luiz
de Castro Brito, Gerly Anne
author_sort de Oliveira, Rodrigo Ribeiro
collection PubMed
description Although of several studies that associate chronic hyperglycemia with tendinopathy, the connection between morphometric changes as witnessed by magnetic resonance (MR) images, nanostructural changes, and inflammatory markers have not yet been fully established. Therefore, the present study has as a hypothesis that the Achilles tendons of rats with diabetes mellitus (DM) exhibit structural changes. The animals were randomly divided into two experimental groups: Control Group (n = 06) injected with a vehicle (sodium citrate buffer solution) and Diabetic Group (n = 06) consisting of rats submitted to intraperitoneal administration of streptozotocin. MR was performed 24 days after the induction of diabetes and images were used for morphometry using ImageJ software. Morphology of the collagen fibers within tendons was examined using Atomic Force microscopy (AFM). An increase in the dimension of the coronal plane area was observed in the diabetic group (8.583 ± 0.646 mm(2)/100g) when compared to the control group (4.823 ± 0.267 mm(2)/100g) resulting in a significant difference (p = 0.003) upon evaluating the Achilles tendons. Similarly, our analysis found an increase in the size of the transverse section area in the diabetic group (1.328 ± 0.103 mm(2)/100g) in comparison to the control group (0.940 ± 0.01 mm(2)/100g) p = 0.021. The tendons of the diabetic group showed great irregularity in fiber bundles, including modified grain direction and jagged junctions and deformities in the form of collagen fibrils bulges. Despite the morphological changes observed in the Achilles tendon of diabetic animals, IL1 and TNF-α did not change. Our results suggest that DM promotes changes to the Achilles tendon with important structural modifications as seen by MR and AFM, excluding major inflammatory changes.
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spelling pubmed-52409622017-02-06 Experimental Diabetes Alters the Morphology and Nano-Structure of the Achilles Tendon de Oliveira, Rodrigo Ribeiro Medina de Mattos, Rômulo Magalhães Rebelo, Luciana Guimarães Meireles Ferreira, Fernanda Tovar-Moll, Fernanda Eurico Nasciutti, Luiz de Castro Brito, Gerly Anne PLoS One Research Article Although of several studies that associate chronic hyperglycemia with tendinopathy, the connection between morphometric changes as witnessed by magnetic resonance (MR) images, nanostructural changes, and inflammatory markers have not yet been fully established. Therefore, the present study has as a hypothesis that the Achilles tendons of rats with diabetes mellitus (DM) exhibit structural changes. The animals were randomly divided into two experimental groups: Control Group (n = 06) injected with a vehicle (sodium citrate buffer solution) and Diabetic Group (n = 06) consisting of rats submitted to intraperitoneal administration of streptozotocin. MR was performed 24 days after the induction of diabetes and images were used for morphometry using ImageJ software. Morphology of the collagen fibers within tendons was examined using Atomic Force microscopy (AFM). An increase in the dimension of the coronal plane area was observed in the diabetic group (8.583 ± 0.646 mm(2)/100g) when compared to the control group (4.823 ± 0.267 mm(2)/100g) resulting in a significant difference (p = 0.003) upon evaluating the Achilles tendons. Similarly, our analysis found an increase in the size of the transverse section area in the diabetic group (1.328 ± 0.103 mm(2)/100g) in comparison to the control group (0.940 ± 0.01 mm(2)/100g) p = 0.021. The tendons of the diabetic group showed great irregularity in fiber bundles, including modified grain direction and jagged junctions and deformities in the form of collagen fibrils bulges. Despite the morphological changes observed in the Achilles tendon of diabetic animals, IL1 and TNF-α did not change. Our results suggest that DM promotes changes to the Achilles tendon with important structural modifications as seen by MR and AFM, excluding major inflammatory changes. Public Library of Science 2017-01-17 /pmc/articles/PMC5240962/ /pubmed/28095484 http://dx.doi.org/10.1371/journal.pone.0169513 Text en © 2017 Oliveira et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
de Oliveira, Rodrigo Ribeiro
Medina de Mattos, Rômulo
Magalhães Rebelo, Luciana
Guimarães Meireles Ferreira, Fernanda
Tovar-Moll, Fernanda
Eurico Nasciutti, Luiz
de Castro Brito, Gerly Anne
Experimental Diabetes Alters the Morphology and Nano-Structure of the Achilles Tendon
title Experimental Diabetes Alters the Morphology and Nano-Structure of the Achilles Tendon
title_full Experimental Diabetes Alters the Morphology and Nano-Structure of the Achilles Tendon
title_fullStr Experimental Diabetes Alters the Morphology and Nano-Structure of the Achilles Tendon
title_full_unstemmed Experimental Diabetes Alters the Morphology and Nano-Structure of the Achilles Tendon
title_short Experimental Diabetes Alters the Morphology and Nano-Structure of the Achilles Tendon
title_sort experimental diabetes alters the morphology and nano-structure of the achilles tendon
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5240962/
https://www.ncbi.nlm.nih.gov/pubmed/28095484
http://dx.doi.org/10.1371/journal.pone.0169513
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