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Experimental Diabetes Alters the Morphology and Nano-Structure of the Achilles Tendon
Although of several studies that associate chronic hyperglycemia with tendinopathy, the connection between morphometric changes as witnessed by magnetic resonance (MR) images, nanostructural changes, and inflammatory markers have not yet been fully established. Therefore, the present study has as a...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5240962/ https://www.ncbi.nlm.nih.gov/pubmed/28095484 http://dx.doi.org/10.1371/journal.pone.0169513 |
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author | de Oliveira, Rodrigo Ribeiro Medina de Mattos, Rômulo Magalhães Rebelo, Luciana Guimarães Meireles Ferreira, Fernanda Tovar-Moll, Fernanda Eurico Nasciutti, Luiz de Castro Brito, Gerly Anne |
author_facet | de Oliveira, Rodrigo Ribeiro Medina de Mattos, Rômulo Magalhães Rebelo, Luciana Guimarães Meireles Ferreira, Fernanda Tovar-Moll, Fernanda Eurico Nasciutti, Luiz de Castro Brito, Gerly Anne |
author_sort | de Oliveira, Rodrigo Ribeiro |
collection | PubMed |
description | Although of several studies that associate chronic hyperglycemia with tendinopathy, the connection between morphometric changes as witnessed by magnetic resonance (MR) images, nanostructural changes, and inflammatory markers have not yet been fully established. Therefore, the present study has as a hypothesis that the Achilles tendons of rats with diabetes mellitus (DM) exhibit structural changes. The animals were randomly divided into two experimental groups: Control Group (n = 06) injected with a vehicle (sodium citrate buffer solution) and Diabetic Group (n = 06) consisting of rats submitted to intraperitoneal administration of streptozotocin. MR was performed 24 days after the induction of diabetes and images were used for morphometry using ImageJ software. Morphology of the collagen fibers within tendons was examined using Atomic Force microscopy (AFM). An increase in the dimension of the coronal plane area was observed in the diabetic group (8.583 ± 0.646 mm(2)/100g) when compared to the control group (4.823 ± 0.267 mm(2)/100g) resulting in a significant difference (p = 0.003) upon evaluating the Achilles tendons. Similarly, our analysis found an increase in the size of the transverse section area in the diabetic group (1.328 ± 0.103 mm(2)/100g) in comparison to the control group (0.940 ± 0.01 mm(2)/100g) p = 0.021. The tendons of the diabetic group showed great irregularity in fiber bundles, including modified grain direction and jagged junctions and deformities in the form of collagen fibrils bulges. Despite the morphological changes observed in the Achilles tendon of diabetic animals, IL1 and TNF-α did not change. Our results suggest that DM promotes changes to the Achilles tendon with important structural modifications as seen by MR and AFM, excluding major inflammatory changes. |
format | Online Article Text |
id | pubmed-5240962 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-52409622017-02-06 Experimental Diabetes Alters the Morphology and Nano-Structure of the Achilles Tendon de Oliveira, Rodrigo Ribeiro Medina de Mattos, Rômulo Magalhães Rebelo, Luciana Guimarães Meireles Ferreira, Fernanda Tovar-Moll, Fernanda Eurico Nasciutti, Luiz de Castro Brito, Gerly Anne PLoS One Research Article Although of several studies that associate chronic hyperglycemia with tendinopathy, the connection between morphometric changes as witnessed by magnetic resonance (MR) images, nanostructural changes, and inflammatory markers have not yet been fully established. Therefore, the present study has as a hypothesis that the Achilles tendons of rats with diabetes mellitus (DM) exhibit structural changes. The animals were randomly divided into two experimental groups: Control Group (n = 06) injected with a vehicle (sodium citrate buffer solution) and Diabetic Group (n = 06) consisting of rats submitted to intraperitoneal administration of streptozotocin. MR was performed 24 days after the induction of diabetes and images were used for morphometry using ImageJ software. Morphology of the collagen fibers within tendons was examined using Atomic Force microscopy (AFM). An increase in the dimension of the coronal plane area was observed in the diabetic group (8.583 ± 0.646 mm(2)/100g) when compared to the control group (4.823 ± 0.267 mm(2)/100g) resulting in a significant difference (p = 0.003) upon evaluating the Achilles tendons. Similarly, our analysis found an increase in the size of the transverse section area in the diabetic group (1.328 ± 0.103 mm(2)/100g) in comparison to the control group (0.940 ± 0.01 mm(2)/100g) p = 0.021. The tendons of the diabetic group showed great irregularity in fiber bundles, including modified grain direction and jagged junctions and deformities in the form of collagen fibrils bulges. Despite the morphological changes observed in the Achilles tendon of diabetic animals, IL1 and TNF-α did not change. Our results suggest that DM promotes changes to the Achilles tendon with important structural modifications as seen by MR and AFM, excluding major inflammatory changes. Public Library of Science 2017-01-17 /pmc/articles/PMC5240962/ /pubmed/28095484 http://dx.doi.org/10.1371/journal.pone.0169513 Text en © 2017 Oliveira et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article de Oliveira, Rodrigo Ribeiro Medina de Mattos, Rômulo Magalhães Rebelo, Luciana Guimarães Meireles Ferreira, Fernanda Tovar-Moll, Fernanda Eurico Nasciutti, Luiz de Castro Brito, Gerly Anne Experimental Diabetes Alters the Morphology and Nano-Structure of the Achilles Tendon |
title | Experimental Diabetes Alters the Morphology and Nano-Structure of the Achilles Tendon |
title_full | Experimental Diabetes Alters the Morphology and Nano-Structure of the Achilles Tendon |
title_fullStr | Experimental Diabetes Alters the Morphology and Nano-Structure of the Achilles Tendon |
title_full_unstemmed | Experimental Diabetes Alters the Morphology and Nano-Structure of the Achilles Tendon |
title_short | Experimental Diabetes Alters the Morphology and Nano-Structure of the Achilles Tendon |
title_sort | experimental diabetes alters the morphology and nano-structure of the achilles tendon |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5240962/ https://www.ncbi.nlm.nih.gov/pubmed/28095484 http://dx.doi.org/10.1371/journal.pone.0169513 |
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