Cargando…
Adipose-Derived Stem Cells Cocultured with Chondrocytes Promote the Proliferation of Chondrocytes
Articular cartilage injury and defect caused by trauma and chronic osteoarthritis vascularity are very common, while the repair of injured cartilage remains a great challenge due to its limited healing capacity. Stem cell-based tissue engineering provides a promising treatment option for injured art...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5241498/ https://www.ncbi.nlm.nih.gov/pubmed/28133485 http://dx.doi.org/10.1155/2017/1709582 |
_version_ | 1782496193718255616 |
---|---|
author | Shi, Jie Liang, Jiulong Guo, Bingyu Zhang, Yu Hui, Qiang Chang, Peng Tao, Kai |
author_facet | Shi, Jie Liang, Jiulong Guo, Bingyu Zhang, Yu Hui, Qiang Chang, Peng Tao, Kai |
author_sort | Shi, Jie |
collection | PubMed |
description | Articular cartilage injury and defect caused by trauma and chronic osteoarthritis vascularity are very common, while the repair of injured cartilage remains a great challenge due to its limited healing capacity. Stem cell-based tissue engineering provides a promising treatment option for injured articular cartilage because of the cells potential for multiple differentiations. However, its application has been largely limited by stem cell type, number, source, proliferation, and differentiation. We hypothesized that (1) adipose-derived stem cells are ideal seed cells for articular cartilage repair because of their accessibility and abundance and (2) the microenvironment of articular cartilage could induce adipose-derived stem cells (ADSCs) to differentiate into chondrocytes. In order to test our hypotheses, we isolated stem cells from rabbit adipose tissues and cocultured these ADSCs with rabbit articular cartilage chondrocytes. We found that when ADSCs were cocultured with chondrocytes, the proliferation of articular cartilage chondrocytes was promoted, the apoptosis of chondrocytes was inhibited, and the osteogenic and chondrogenic differentiation of ADSCs was enhanced. The study on the mechanism of this coculture system indicated that the role of this coculture system is similar to the function of TGF-β1 in the promotion of chondrocytes. |
format | Online Article Text |
id | pubmed-5241498 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-52414982017-01-29 Adipose-Derived Stem Cells Cocultured with Chondrocytes Promote the Proliferation of Chondrocytes Shi, Jie Liang, Jiulong Guo, Bingyu Zhang, Yu Hui, Qiang Chang, Peng Tao, Kai Stem Cells Int Research Article Articular cartilage injury and defect caused by trauma and chronic osteoarthritis vascularity are very common, while the repair of injured cartilage remains a great challenge due to its limited healing capacity. Stem cell-based tissue engineering provides a promising treatment option for injured articular cartilage because of the cells potential for multiple differentiations. However, its application has been largely limited by stem cell type, number, source, proliferation, and differentiation. We hypothesized that (1) adipose-derived stem cells are ideal seed cells for articular cartilage repair because of their accessibility and abundance and (2) the microenvironment of articular cartilage could induce adipose-derived stem cells (ADSCs) to differentiate into chondrocytes. In order to test our hypotheses, we isolated stem cells from rabbit adipose tissues and cocultured these ADSCs with rabbit articular cartilage chondrocytes. We found that when ADSCs were cocultured with chondrocytes, the proliferation of articular cartilage chondrocytes was promoted, the apoptosis of chondrocytes was inhibited, and the osteogenic and chondrogenic differentiation of ADSCs was enhanced. The study on the mechanism of this coculture system indicated that the role of this coculture system is similar to the function of TGF-β1 in the promotion of chondrocytes. Hindawi Publishing Corporation 2017 2017-01-04 /pmc/articles/PMC5241498/ /pubmed/28133485 http://dx.doi.org/10.1155/2017/1709582 Text en Copyright © 2017 Jie Shi et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Shi, Jie Liang, Jiulong Guo, Bingyu Zhang, Yu Hui, Qiang Chang, Peng Tao, Kai Adipose-Derived Stem Cells Cocultured with Chondrocytes Promote the Proliferation of Chondrocytes |
title | Adipose-Derived Stem Cells Cocultured with Chondrocytes Promote the Proliferation of Chondrocytes |
title_full | Adipose-Derived Stem Cells Cocultured with Chondrocytes Promote the Proliferation of Chondrocytes |
title_fullStr | Adipose-Derived Stem Cells Cocultured with Chondrocytes Promote the Proliferation of Chondrocytes |
title_full_unstemmed | Adipose-Derived Stem Cells Cocultured with Chondrocytes Promote the Proliferation of Chondrocytes |
title_short | Adipose-Derived Stem Cells Cocultured with Chondrocytes Promote the Proliferation of Chondrocytes |
title_sort | adipose-derived stem cells cocultured with chondrocytes promote the proliferation of chondrocytes |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5241498/ https://www.ncbi.nlm.nih.gov/pubmed/28133485 http://dx.doi.org/10.1155/2017/1709582 |
work_keys_str_mv | AT shijie adiposederivedstemcellscoculturedwithchondrocytespromotetheproliferationofchondrocytes AT liangjiulong adiposederivedstemcellscoculturedwithchondrocytespromotetheproliferationofchondrocytes AT guobingyu adiposederivedstemcellscoculturedwithchondrocytespromotetheproliferationofchondrocytes AT zhangyu adiposederivedstemcellscoculturedwithchondrocytespromotetheproliferationofchondrocytes AT huiqiang adiposederivedstemcellscoculturedwithchondrocytespromotetheproliferationofchondrocytes AT changpeng adiposederivedstemcellscoculturedwithchondrocytespromotetheproliferationofchondrocytes AT taokai adiposederivedstemcellscoculturedwithchondrocytespromotetheproliferationofchondrocytes |