Profiling single-guide RNA specificity reveals a mismatch sensitive core sequence

Targeting specificity is an essential issue in the development of CRISPR-Cas technology. Using a luciferase activation assay, off-target cleavage activity of sgRNA was systematically investigated on single nucleotide-mismatched targets. In addition to confirming that PAM-proximal mismatches are less...

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Detalles Bibliográficos
Autores principales: Zheng, Ting, Hou, Yingzi, Zhang, Pingjing, Zhang, Zhenxi, Xu, Ying, Zhang, Letian, Niu, Leilei, Yang, Yi, Liang, Da, Yi, Fan, Peng, Wei, Feng, Wenjian, Yang, Ying, Chen, Jianxin, Zhu, York Yuanyuan, Zhang, Li-He, Du, Quan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5241822/
https://www.ncbi.nlm.nih.gov/pubmed/28098181
http://dx.doi.org/10.1038/srep40638

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