Cargando…
Crystal structure of Sec10, a subunit of the exocyst complex
The exocyst complex is a heterooctameric protein complex composed of Sec3, Sec5, Sec6, Sec8, Sec10, Sec15, Exo70 and Exo84. This complex plays an essential role in trafficking secretory vesicles to the plasma membrane through its interaction with phosphatidylinositol 4,5-bisphosphate and small GTPas...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5241887/ https://www.ncbi.nlm.nih.gov/pubmed/28098232 http://dx.doi.org/10.1038/srep40909 |
_version_ | 1782496258416443392 |
---|---|
author | Chen, Jianxing Yamagata, Atsushi Kubota, Keiko Sato, Yusuke Goto-Ito, Sakurako Fukai, Shuya |
author_facet | Chen, Jianxing Yamagata, Atsushi Kubota, Keiko Sato, Yusuke Goto-Ito, Sakurako Fukai, Shuya |
author_sort | Chen, Jianxing |
collection | PubMed |
description | The exocyst complex is a heterooctameric protein complex composed of Sec3, Sec5, Sec6, Sec8, Sec10, Sec15, Exo70 and Exo84. This complex plays an essential role in trafficking secretory vesicles to the plasma membrane through its interaction with phosphatidylinositol 4,5-bisphosphate and small GTPases. To date, the near-full-length structural information of each subunit has been limited to Exo70, although the C-terminal half structures of Sec6, Sec15 and Exo84 and the structures of the small GTPase-binding domains of Sec3, Sec5 and Exo84 have been reported. Here, we report the crystal structure of the near-full-length zebrafish Sec10 (zSec10) at 2.73 Å resolution. The structure of zSec10 consists of tandem antiparallel helix bundles that form a straight rod, like helical core regions of other exocyst subunits. This structure provides the first atomic details of Sec10, which may be useful for future functional and structural studies of this subunit and the exocyst complex. |
format | Online Article Text |
id | pubmed-5241887 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-52418872017-01-23 Crystal structure of Sec10, a subunit of the exocyst complex Chen, Jianxing Yamagata, Atsushi Kubota, Keiko Sato, Yusuke Goto-Ito, Sakurako Fukai, Shuya Sci Rep Article The exocyst complex is a heterooctameric protein complex composed of Sec3, Sec5, Sec6, Sec8, Sec10, Sec15, Exo70 and Exo84. This complex plays an essential role in trafficking secretory vesicles to the plasma membrane through its interaction with phosphatidylinositol 4,5-bisphosphate and small GTPases. To date, the near-full-length structural information of each subunit has been limited to Exo70, although the C-terminal half structures of Sec6, Sec15 and Exo84 and the structures of the small GTPase-binding domains of Sec3, Sec5 and Exo84 have been reported. Here, we report the crystal structure of the near-full-length zebrafish Sec10 (zSec10) at 2.73 Å resolution. The structure of zSec10 consists of tandem antiparallel helix bundles that form a straight rod, like helical core regions of other exocyst subunits. This structure provides the first atomic details of Sec10, which may be useful for future functional and structural studies of this subunit and the exocyst complex. Nature Publishing Group 2017-01-18 /pmc/articles/PMC5241887/ /pubmed/28098232 http://dx.doi.org/10.1038/srep40909 Text en Copyright © 2017, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Chen, Jianxing Yamagata, Atsushi Kubota, Keiko Sato, Yusuke Goto-Ito, Sakurako Fukai, Shuya Crystal structure of Sec10, a subunit of the exocyst complex |
title | Crystal structure of Sec10, a subunit of the exocyst complex |
title_full | Crystal structure of Sec10, a subunit of the exocyst complex |
title_fullStr | Crystal structure of Sec10, a subunit of the exocyst complex |
title_full_unstemmed | Crystal structure of Sec10, a subunit of the exocyst complex |
title_short | Crystal structure of Sec10, a subunit of the exocyst complex |
title_sort | crystal structure of sec10, a subunit of the exocyst complex |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5241887/ https://www.ncbi.nlm.nih.gov/pubmed/28098232 http://dx.doi.org/10.1038/srep40909 |
work_keys_str_mv | AT chenjianxing crystalstructureofsec10asubunitoftheexocystcomplex AT yamagataatsushi crystalstructureofsec10asubunitoftheexocystcomplex AT kubotakeiko crystalstructureofsec10asubunitoftheexocystcomplex AT satoyusuke crystalstructureofsec10asubunitoftheexocystcomplex AT gotoitosakurako crystalstructureofsec10asubunitoftheexocystcomplex AT fukaishuya crystalstructureofsec10asubunitoftheexocystcomplex |