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The Analysis of Physiological Variations in M(2) Generation of Solanum melongena L. Mutagenized by Ethyl Methane Sulfonate

The eggplant was mutagenized with ethyl methane sulfonate (EMS) to enhance its genetic variability in our previous paper. In this article, we further analyzed the phenotype of M(2) generation of mutant eggplants. A total of 325 independent M(2) families were investigated for phenotypic variation. In...

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Autores principales: Xi-ou, Xiao, Wenqiu, Lin, Wei, Li, Xiaoming, Gao, Lingling, Lv, Feiyue, Ma, Yuge, Liu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5243811/
https://www.ncbi.nlm.nih.gov/pubmed/28154575
http://dx.doi.org/10.3389/fpls.2017.00017
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author Xi-ou, Xiao
Wenqiu, Lin
Wei, Li
Xiaoming, Gao
Lingling, Lv
Feiyue, Ma
Yuge, Liu
author_facet Xi-ou, Xiao
Wenqiu, Lin
Wei, Li
Xiaoming, Gao
Lingling, Lv
Feiyue, Ma
Yuge, Liu
author_sort Xi-ou, Xiao
collection PubMed
description The eggplant was mutagenized with ethyl methane sulfonate (EMS) to enhance its genetic variability in our previous paper. In this article, we further analyzed the phenotype of M(2) generation of mutant eggplants. A total of 325 independent M(2) families were investigated for phenotypic variation. In addition to the visible phenotypic variation, chlorogenic acid (CGA) concentrations were analyzed in 26 fruits of mutants with High Performance Liquid Chromatography assay. Seventeen fruits exhibited significantly higher concentrations of CGAs than those in wild-type. The anthocyanin concentration of S9-1, the purple black mutant, was higher than WT, meanwhile, the anthocyanin concentration of L6-4 and U36-1 was lower than WT. Furthermore, our RT-PCR result demonstrated that the expression levels of anthocyanin biosynthetic genes, except for SmPAL, were increased in S9-1, and the regulator SmMYB1 was decreased in L6-4 and U36-1 mutants. Together, our data indicated that, M(2) generation showed abundant phenotypic variations and the strong potential usage for next step of breeding and molecular genetic mechanisms in eggplant.
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spelling pubmed-52438112017-02-02 The Analysis of Physiological Variations in M(2) Generation of Solanum melongena L. Mutagenized by Ethyl Methane Sulfonate Xi-ou, Xiao Wenqiu, Lin Wei, Li Xiaoming, Gao Lingling, Lv Feiyue, Ma Yuge, Liu Front Plant Sci Plant Science The eggplant was mutagenized with ethyl methane sulfonate (EMS) to enhance its genetic variability in our previous paper. In this article, we further analyzed the phenotype of M(2) generation of mutant eggplants. A total of 325 independent M(2) families were investigated for phenotypic variation. In addition to the visible phenotypic variation, chlorogenic acid (CGA) concentrations were analyzed in 26 fruits of mutants with High Performance Liquid Chromatography assay. Seventeen fruits exhibited significantly higher concentrations of CGAs than those in wild-type. The anthocyanin concentration of S9-1, the purple black mutant, was higher than WT, meanwhile, the anthocyanin concentration of L6-4 and U36-1 was lower than WT. Furthermore, our RT-PCR result demonstrated that the expression levels of anthocyanin biosynthetic genes, except for SmPAL, were increased in S9-1, and the regulator SmMYB1 was decreased in L6-4 and U36-1 mutants. Together, our data indicated that, M(2) generation showed abundant phenotypic variations and the strong potential usage for next step of breeding and molecular genetic mechanisms in eggplant. Frontiers Media S.A. 2017-01-19 /pmc/articles/PMC5243811/ /pubmed/28154575 http://dx.doi.org/10.3389/fpls.2017.00017 Text en Copyright © 2017 Xi-ou, Wenqiu, Wei, Xiaomin, Lingling, Feiyue and Yuge. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Xi-ou, Xiao
Wenqiu, Lin
Wei, Li
Xiaoming, Gao
Lingling, Lv
Feiyue, Ma
Yuge, Liu
The Analysis of Physiological Variations in M(2) Generation of Solanum melongena L. Mutagenized by Ethyl Methane Sulfonate
title The Analysis of Physiological Variations in M(2) Generation of Solanum melongena L. Mutagenized by Ethyl Methane Sulfonate
title_full The Analysis of Physiological Variations in M(2) Generation of Solanum melongena L. Mutagenized by Ethyl Methane Sulfonate
title_fullStr The Analysis of Physiological Variations in M(2) Generation of Solanum melongena L. Mutagenized by Ethyl Methane Sulfonate
title_full_unstemmed The Analysis of Physiological Variations in M(2) Generation of Solanum melongena L. Mutagenized by Ethyl Methane Sulfonate
title_short The Analysis of Physiological Variations in M(2) Generation of Solanum melongena L. Mutagenized by Ethyl Methane Sulfonate
title_sort analysis of physiological variations in m(2) generation of solanum melongena l. mutagenized by ethyl methane sulfonate
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5243811/
https://www.ncbi.nlm.nih.gov/pubmed/28154575
http://dx.doi.org/10.3389/fpls.2017.00017
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