Generation of Spheres from Dental Epithelial Stem Cells

The in vitro three-dimensional sphere model has already been established as an important tool in fundamental sciences. This model facilitates the study of a variety of biological processes including stem cell/niche functions and tissue responses to injury and drugs. Here we describe the complete pro...

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Autores principales: Natsiou, Despoina, Granchi, Zoraide, Mitsiadis, Thimios A., Jimenez-Rojo, Lucia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2017
Materias:
Physiology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5243826/
https://www.ncbi.nlm.nih.gov/pubmed/28154538
http://dx.doi.org/10.3389/fphys.2017.00007
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author Natsiou, Despoina
Granchi, Zoraide
Mitsiadis, Thimios A.
Jimenez-Rojo, Lucia
author_facet Natsiou, Despoina
Granchi, Zoraide
Mitsiadis, Thimios A.
Jimenez-Rojo, Lucia
author_sort Natsiou, Despoina
collection PubMed
description The in vitro three-dimensional sphere model has already been established as an important tool in fundamental sciences. This model facilitates the study of a variety of biological processes including stem cell/niche functions and tissue responses to injury and drugs. Here we describe the complete protocol for the in vitro formation of spheres originated from the epithelium of rodent incisors. In addition, we show that in these spheres cell proliferation is maintained, as well as the expression of several key molecules characterizing stem cells such as Sox2 and p63. These epithelial dentospheres could be used as an in vitro model system for stem cell research purposes.
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spelling pubmed-52438262017-02-02 Generation of Spheres from Dental Epithelial Stem Cells Natsiou, Despoina Granchi, Zoraide Mitsiadis, Thimios A. Jimenez-Rojo, Lucia Front Physiol Physiology The in vitro three-dimensional sphere model has already been established as an important tool in fundamental sciences. This model facilitates the study of a variety of biological processes including stem cell/niche functions and tissue responses to injury and drugs. Here we describe the complete protocol for the in vitro formation of spheres originated from the epithelium of rodent incisors. In addition, we show that in these spheres cell proliferation is maintained, as well as the expression of several key molecules characterizing stem cells such as Sox2 and p63. These epithelial dentospheres could be used as an in vitro model system for stem cell research purposes. Frontiers Media S.A. 2017-01-19 /pmc/articles/PMC5243826/ /pubmed/28154538 http://dx.doi.org/10.3389/fphys.2017.00007 Text en Copyright © 2017 Natsiou, Granchi, Mitsiadis and Jimenez-Rojo. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Physiology
Natsiou, Despoina
Granchi, Zoraide
Mitsiadis, Thimios A.
Jimenez-Rojo, Lucia
Generation of Spheres from Dental Epithelial Stem Cells
title Generation of Spheres from Dental Epithelial Stem Cells
title_full Generation of Spheres from Dental Epithelial Stem Cells
title_fullStr Generation of Spheres from Dental Epithelial Stem Cells
title_full_unstemmed Generation of Spheres from Dental Epithelial Stem Cells
title_short Generation of Spheres from Dental Epithelial Stem Cells
title_sort generation of spheres from dental epithelial stem cells
topic Physiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5243826/
https://www.ncbi.nlm.nih.gov/pubmed/28154538
http://dx.doi.org/10.3389/fphys.2017.00007
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