Cargando…

Genome-wide identification, characterisation and expression profiles of calcium-dependent protein kinase genes in barley (Hordeum vulgare L.)

In plant cells, calcium-dependent protein kinases (CDPKs) are important sensors of Ca(2+) flux resulting from various environmental stresses like cold, drought or salt stress. Previous genome sequence analysis and comparative studies in Arabidopsis (Arabidopsis thaliana L.) and rice (Oryza sativa L....

Descripción completa

Detalles Bibliográficos
Autores principales: Fedorowicz-Strońska, Olga, Koczyk, Grzegorz, Kaczmarek, Małgorzata, Krajewski, Paweł, Sadowski, Jan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5243917/
https://www.ncbi.nlm.nih.gov/pubmed/27447459
http://dx.doi.org/10.1007/s13353-016-0357-2
_version_ 1782496602962788352
author Fedorowicz-Strońska, Olga
Koczyk, Grzegorz
Kaczmarek, Małgorzata
Krajewski, Paweł
Sadowski, Jan
author_facet Fedorowicz-Strońska, Olga
Koczyk, Grzegorz
Kaczmarek, Małgorzata
Krajewski, Paweł
Sadowski, Jan
author_sort Fedorowicz-Strońska, Olga
collection PubMed
description In plant cells, calcium-dependent protein kinases (CDPKs) are important sensors of Ca(2+) flux resulting from various environmental stresses like cold, drought or salt stress. Previous genome sequence analysis and comparative studies in Arabidopsis (Arabidopsis thaliana L.) and rice (Oryza sativa L.) defined a multi-gene family of CDPKs. Here, we identified and characterised the CDPK gene complement of the model plant, barley (Hordeum vulgare L.). Comparative analysis encompassed phylogeny reconstruction based on newly available barley genome sequence, as well as established model genomes (e.g. O. sativa, A. thaliana, Brachypodium distachyon). Functional gene copies possessed characteristic CDPK domain architecture, including a serine/threonine kinase domain and four regulatory EF-hand motifs. In silico verification was followed by measurements of transcript abundance via real-time polymerase chain reaction (PCR). The relative expression of CDPK genes was determined in the vegetative growth stage under intensifying drought stress conditions. The majority of barley CDPK genes showed distinct changes in patterns of expression during exposure to stress. Our study constitutes evidence for involvement of the barley CDPK gene complement in signal transduction pathways relating to adaptation to drought. Our bioinformatics and transcriptomic analyses will provide an important foundation for further functional dissection of the barley CDPK gene family. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s13353-016-0357-2) contains supplementary material, which is available to authorized users.
format Online
Article
Text
id pubmed-5243917
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Springer Berlin Heidelberg
record_format MEDLINE/PubMed
spelling pubmed-52439172017-02-01 Genome-wide identification, characterisation and expression profiles of calcium-dependent protein kinase genes in barley (Hordeum vulgare L.) Fedorowicz-Strońska, Olga Koczyk, Grzegorz Kaczmarek, Małgorzata Krajewski, Paweł Sadowski, Jan J Appl Genet Plant Genetics • Original Paper In plant cells, calcium-dependent protein kinases (CDPKs) are important sensors of Ca(2+) flux resulting from various environmental stresses like cold, drought or salt stress. Previous genome sequence analysis and comparative studies in Arabidopsis (Arabidopsis thaliana L.) and rice (Oryza sativa L.) defined a multi-gene family of CDPKs. Here, we identified and characterised the CDPK gene complement of the model plant, barley (Hordeum vulgare L.). Comparative analysis encompassed phylogeny reconstruction based on newly available barley genome sequence, as well as established model genomes (e.g. O. sativa, A. thaliana, Brachypodium distachyon). Functional gene copies possessed characteristic CDPK domain architecture, including a serine/threonine kinase domain and four regulatory EF-hand motifs. In silico verification was followed by measurements of transcript abundance via real-time polymerase chain reaction (PCR). The relative expression of CDPK genes was determined in the vegetative growth stage under intensifying drought stress conditions. The majority of barley CDPK genes showed distinct changes in patterns of expression during exposure to stress. Our study constitutes evidence for involvement of the barley CDPK gene complement in signal transduction pathways relating to adaptation to drought. Our bioinformatics and transcriptomic analyses will provide an important foundation for further functional dissection of the barley CDPK gene family. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s13353-016-0357-2) contains supplementary material, which is available to authorized users. Springer Berlin Heidelberg 2016-07-22 2017 /pmc/articles/PMC5243917/ /pubmed/27447459 http://dx.doi.org/10.1007/s13353-016-0357-2 Text en © The Author(s) 2016 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Plant Genetics • Original Paper
Fedorowicz-Strońska, Olga
Koczyk, Grzegorz
Kaczmarek, Małgorzata
Krajewski, Paweł
Sadowski, Jan
Genome-wide identification, characterisation and expression profiles of calcium-dependent protein kinase genes in barley (Hordeum vulgare L.)
title Genome-wide identification, characterisation and expression profiles of calcium-dependent protein kinase genes in barley (Hordeum vulgare L.)
title_full Genome-wide identification, characterisation and expression profiles of calcium-dependent protein kinase genes in barley (Hordeum vulgare L.)
title_fullStr Genome-wide identification, characterisation and expression profiles of calcium-dependent protein kinase genes in barley (Hordeum vulgare L.)
title_full_unstemmed Genome-wide identification, characterisation and expression profiles of calcium-dependent protein kinase genes in barley (Hordeum vulgare L.)
title_short Genome-wide identification, characterisation and expression profiles of calcium-dependent protein kinase genes in barley (Hordeum vulgare L.)
title_sort genome-wide identification, characterisation and expression profiles of calcium-dependent protein kinase genes in barley (hordeum vulgare l.)
topic Plant Genetics • Original Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5243917/
https://www.ncbi.nlm.nih.gov/pubmed/27447459
http://dx.doi.org/10.1007/s13353-016-0357-2
work_keys_str_mv AT fedorowiczstronskaolga genomewideidentificationcharacterisationandexpressionprofilesofcalciumdependentproteinkinasegenesinbarleyhordeumvulgarel
AT koczykgrzegorz genomewideidentificationcharacterisationandexpressionprofilesofcalciumdependentproteinkinasegenesinbarleyhordeumvulgarel
AT kaczmarekmałgorzata genomewideidentificationcharacterisationandexpressionprofilesofcalciumdependentproteinkinasegenesinbarleyhordeumvulgarel
AT krajewskipaweł genomewideidentificationcharacterisationandexpressionprofilesofcalciumdependentproteinkinasegenesinbarleyhordeumvulgarel
AT sadowskijan genomewideidentificationcharacterisationandexpressionprofilesofcalciumdependentproteinkinasegenesinbarleyhordeumvulgarel