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Mathematical Model for Predicting the Growth Probability of Staphylococcus aureus in Combinations of NaCl and NaNO(2) under Aerobic or Evacuated Storage Conditions
The objective of this study was to describe the growth patterns of Staphylococcus aureus in combinations of NaCl and NaNO(2), using a probabilistic model. A mixture of S. aureus strains (NCCP10826, ATCC13565, ATCC14458, ATCC23235, and ATCC27664) was inoculated into nutrient broth plus NaCl (0, 0.25,...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Korean Society for Food Science of Animal Resources
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5243959/ https://www.ncbi.nlm.nih.gov/pubmed/28115886 http://dx.doi.org/10.5851/kosfa.2016.36.6.752 |
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author | Lee, Jeeyeon Gwak, Eunji Ha, Jimyeong Kim, Sejeong Lee, Soomin Lee, Heeyoung Oh, Mi-Hwa Park, Beom-Young Oh, Nam Su Choi, Kyoung-Hee Yoon, Yohan |
author_facet | Lee, Jeeyeon Gwak, Eunji Ha, Jimyeong Kim, Sejeong Lee, Soomin Lee, Heeyoung Oh, Mi-Hwa Park, Beom-Young Oh, Nam Su Choi, Kyoung-Hee Yoon, Yohan |
author_sort | Lee, Jeeyeon |
collection | PubMed |
description | The objective of this study was to describe the growth patterns of Staphylococcus aureus in combinations of NaCl and NaNO(2), using a probabilistic model. A mixture of S. aureus strains (NCCP10826, ATCC13565, ATCC14458, ATCC23235, and ATCC27664) was inoculated into nutrient broth plus NaCl (0, 0.25, 0.5, 0.75, 1, 1.25, 1.5, and 1.75%) and NaNO(2) (0, 15, 30, 45, 60, 75, 90, 105, and 120 ppm). The samples were then incubated at 4, 7, 10, 12 and 15℃ for up to 60 d under aerobic or vacuum conditions. Growth responses [growth (1) or no growth (0)] were then determined every 24 h by turbidity, and analyzed to select significant parameters (p<0.05) by a stepwise selection method, resulting in a probabilistic model. The developed models were then validated with observed growth responses. S. aureus growth was observed only under aerobic storage at 10-15℃. At 10-15℃, NaCl and NaNO(2) did not inhibit S. aureus growth at less than 1.25% NaCl. Concentration dependency was observed for NaCl at more than 1.25%, but not for NaNO(2). The concordance percentage between observed and predicted growth data was approximately 93.86%. This result indicates that S. aureus growth can be inhibited in vacuum packaging and even aerobic storage below 10℃. Furthermore, NaNO(2) does not effectively inhibit S. aureus growth. |
format | Online Article Text |
id | pubmed-5243959 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Korean Society for Food Science of Animal Resources |
record_format | MEDLINE/PubMed |
spelling | pubmed-52439592017-01-23 Mathematical Model for Predicting the Growth Probability of Staphylococcus aureus in Combinations of NaCl and NaNO(2) under Aerobic or Evacuated Storage Conditions Lee, Jeeyeon Gwak, Eunji Ha, Jimyeong Kim, Sejeong Lee, Soomin Lee, Heeyoung Oh, Mi-Hwa Park, Beom-Young Oh, Nam Su Choi, Kyoung-Hee Yoon, Yohan Korean J Food Sci Anim Resour Article The objective of this study was to describe the growth patterns of Staphylococcus aureus in combinations of NaCl and NaNO(2), using a probabilistic model. A mixture of S. aureus strains (NCCP10826, ATCC13565, ATCC14458, ATCC23235, and ATCC27664) was inoculated into nutrient broth plus NaCl (0, 0.25, 0.5, 0.75, 1, 1.25, 1.5, and 1.75%) and NaNO(2) (0, 15, 30, 45, 60, 75, 90, 105, and 120 ppm). The samples were then incubated at 4, 7, 10, 12 and 15℃ for up to 60 d under aerobic or vacuum conditions. Growth responses [growth (1) or no growth (0)] were then determined every 24 h by turbidity, and analyzed to select significant parameters (p<0.05) by a stepwise selection method, resulting in a probabilistic model. The developed models were then validated with observed growth responses. S. aureus growth was observed only under aerobic storage at 10-15℃. At 10-15℃, NaCl and NaNO(2) did not inhibit S. aureus growth at less than 1.25% NaCl. Concentration dependency was observed for NaCl at more than 1.25%, but not for NaNO(2). The concordance percentage between observed and predicted growth data was approximately 93.86%. This result indicates that S. aureus growth can be inhibited in vacuum packaging and even aerobic storage below 10℃. Furthermore, NaNO(2) does not effectively inhibit S. aureus growth. Korean Society for Food Science of Animal Resources 2016 2016-12-31 /pmc/articles/PMC5243959/ /pubmed/28115886 http://dx.doi.org/10.5851/kosfa.2016.36.6.752 Text en Copyright © 2016, Korean Society for Food Science of Animal Resources This is an open access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licences/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Article Lee, Jeeyeon Gwak, Eunji Ha, Jimyeong Kim, Sejeong Lee, Soomin Lee, Heeyoung Oh, Mi-Hwa Park, Beom-Young Oh, Nam Su Choi, Kyoung-Hee Yoon, Yohan Mathematical Model for Predicting the Growth Probability of Staphylococcus aureus in Combinations of NaCl and NaNO(2) under Aerobic or Evacuated Storage Conditions |
title | Mathematical Model for Predicting the Growth Probability of Staphylococcus aureus in Combinations of NaCl and NaNO(2) under Aerobic or Evacuated Storage Conditions |
title_full | Mathematical Model for Predicting the Growth Probability of Staphylococcus aureus in Combinations of NaCl and NaNO(2) under Aerobic or Evacuated Storage Conditions |
title_fullStr | Mathematical Model for Predicting the Growth Probability of Staphylococcus aureus in Combinations of NaCl and NaNO(2) under Aerobic or Evacuated Storage Conditions |
title_full_unstemmed | Mathematical Model for Predicting the Growth Probability of Staphylococcus aureus in Combinations of NaCl and NaNO(2) under Aerobic or Evacuated Storage Conditions |
title_short | Mathematical Model for Predicting the Growth Probability of Staphylococcus aureus in Combinations of NaCl and NaNO(2) under Aerobic or Evacuated Storage Conditions |
title_sort | mathematical model for predicting the growth probability of staphylococcus aureus in combinations of nacl and nano(2) under aerobic or evacuated storage conditions |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5243959/ https://www.ncbi.nlm.nih.gov/pubmed/28115886 http://dx.doi.org/10.5851/kosfa.2016.36.6.752 |
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