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Prevalence of allergic sensitization to conifer pollen in a high cypress exposure area
BACKGROUND: Sensitization to Pinales (Cupressaceae and Pinaceae) has increased dramatically in recent years. The prevalence of sensitization in different geographic areas is related to exposure to specific pollens. OBJECTIVES: To investigate the prevalence of allergy to different conifer pollens, de...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
OceanSide Publications, Inc.
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5244279/ https://www.ncbi.nlm.nih.gov/pubmed/28683246 http://dx.doi.org/10.2500/ar.2016.7.0183 |
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author | Domínguez-Ortega, Javier López-Matas, María Ángeles Alonso, María Dolores Feliú, Angélica Ruiz-Hornillos, Javier González, Emma Moya, Raquel Carnés, Jerónimo |
author_facet | Domínguez-Ortega, Javier López-Matas, María Ángeles Alonso, María Dolores Feliú, Angélica Ruiz-Hornillos, Javier González, Emma Moya, Raquel Carnés, Jerónimo |
author_sort | Domínguez-Ortega, Javier |
collection | PubMed |
description | BACKGROUND: Sensitization to Pinales (Cupressaceae and Pinaceae) has increased dramatically in recent years. The prevalence of sensitization in different geographic areas is related to exposure to specific pollens. OBJECTIVES: To investigate the prevalence of allergy to different conifer pollens, describe the characteristics of patients with such allergy, and identify the involved allergens. METHODS: Patients were recruited at five hospitals near Madrid. Extracts from conifer pollen were prepared and used in skin-prick testing. Wheal sizes were recorded, and serum samples obtained from patients with positive reactions to Cupressus arizonica and/or Pinus pinea. The specific immunoglobulin E value to C. arizonica and Cup a 1 was determined. Individual immunoblots for each patient and with a pool of sera were performed. Allergenic proteins were sequenced by using liquid chromatography-tandem mass spectrometry. RESULTS: Of 499 individuals included in the study, 17 (14%) had positive skin-prick test results to some conifer pollen extracts. Sixty-four patients had positive results to C. arizonica (prevalence 12.8%) and 11 had positive results to P. pinea (2.2%). All the patients had respiratory symptoms (61.4% during the C. arizonica pollination period), and 62.9% had asthma. Approximately 86% of the patients had positive specific immunoglobulin E results to C. arizonica and 92.3% had positive results to Cup a 1. Fourteen different bands were recognized by immunoblot; the most frequent bands were those detected at 43, 18, 16, and 14 kDa. All sequenced proteins corresponded to Cup a 1. CONCLUSION: Allergy to conifer pollen could be considered a relevant cause of respiratory allergy in central Spain. Asthma was more frequent than in other studies. We only identified Cup a 1 as involved in sensitization. |
format | Online Article Text |
id | pubmed-5244279 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | OceanSide Publications, Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-52442792017-01-23 Prevalence of allergic sensitization to conifer pollen in a high cypress exposure area Domínguez-Ortega, Javier López-Matas, María Ángeles Alonso, María Dolores Feliú, Angélica Ruiz-Hornillos, Javier González, Emma Moya, Raquel Carnés, Jerónimo Allergy Rhinol (Providence) Articles BACKGROUND: Sensitization to Pinales (Cupressaceae and Pinaceae) has increased dramatically in recent years. The prevalence of sensitization in different geographic areas is related to exposure to specific pollens. OBJECTIVES: To investigate the prevalence of allergy to different conifer pollens, describe the characteristics of patients with such allergy, and identify the involved allergens. METHODS: Patients were recruited at five hospitals near Madrid. Extracts from conifer pollen were prepared and used in skin-prick testing. Wheal sizes were recorded, and serum samples obtained from patients with positive reactions to Cupressus arizonica and/or Pinus pinea. The specific immunoglobulin E value to C. arizonica and Cup a 1 was determined. Individual immunoblots for each patient and with a pool of sera were performed. Allergenic proteins were sequenced by using liquid chromatography-tandem mass spectrometry. RESULTS: Of 499 individuals included in the study, 17 (14%) had positive skin-prick test results to some conifer pollen extracts. Sixty-four patients had positive results to C. arizonica (prevalence 12.8%) and 11 had positive results to P. pinea (2.2%). All the patients had respiratory symptoms (61.4% during the C. arizonica pollination period), and 62.9% had asthma. Approximately 86% of the patients had positive specific immunoglobulin E results to C. arizonica and 92.3% had positive results to Cup a 1. Fourteen different bands were recognized by immunoblot; the most frequent bands were those detected at 43, 18, 16, and 14 kDa. All sequenced proteins corresponded to Cup a 1. CONCLUSION: Allergy to conifer pollen could be considered a relevant cause of respiratory allergy in central Spain. Asthma was more frequent than in other studies. We only identified Cup a 1 as involved in sensitization. OceanSide Publications, Inc. 2016 /pmc/articles/PMC5244279/ /pubmed/28683246 http://dx.doi.org/10.2500/ar.2016.7.0183 Text en Copyright © 2016, OceanSide Publications, Inc., U.S.A. This publication is provided under the terms of the Creative Commons Public License ("CCPL" or "License"), in attribution 3.0 unported (Attribution Non-Commercial No Derivatives (CC BY-NC-ND)), further described at: http://creativecommons.org/licenses/by-nc-nd/3.0/legalcode. The work is protected by copyright and/or other applicable law. Any use of the work other then as authorized under this license or copyright law is prohibited. |
spellingShingle | Articles Domínguez-Ortega, Javier López-Matas, María Ángeles Alonso, María Dolores Feliú, Angélica Ruiz-Hornillos, Javier González, Emma Moya, Raquel Carnés, Jerónimo Prevalence of allergic sensitization to conifer pollen in a high cypress exposure area |
title | Prevalence of allergic sensitization to conifer pollen in a high cypress exposure area |
title_full | Prevalence of allergic sensitization to conifer pollen in a high cypress exposure area |
title_fullStr | Prevalence of allergic sensitization to conifer pollen in a high cypress exposure area |
title_full_unstemmed | Prevalence of allergic sensitization to conifer pollen in a high cypress exposure area |
title_short | Prevalence of allergic sensitization to conifer pollen in a high cypress exposure area |
title_sort | prevalence of allergic sensitization to conifer pollen in a high cypress exposure area |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5244279/ https://www.ncbi.nlm.nih.gov/pubmed/28683246 http://dx.doi.org/10.2500/ar.2016.7.0183 |
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