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Tracking of Mesenchymal Stem Cells with Fluorescence Endomicroscopy Imaging in Radiotherapy-Induced Lung Injury

Mesenchymal stem cells (MSCs) have potential for reducing inflammation and promoting organ repair. However, limitations in available techniques to track them and assess this potential for lung repair have hindered their applicability. In this work, we proposed, implemented and evaluated the use of f...

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Autores principales: Perez, Jessica R., Ybarra, Norma, Chagnon, Frederic, Serban, Monica, Lee, Sangkyu, Seuntjens, Jan, Lesur, Olivier, El Naqa, Issam
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5244404/
https://www.ncbi.nlm.nih.gov/pubmed/28102237
http://dx.doi.org/10.1038/srep40748
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author Perez, Jessica R.
Ybarra, Norma
Chagnon, Frederic
Serban, Monica
Lee, Sangkyu
Seuntjens, Jan
Lesur, Olivier
El Naqa, Issam
author_facet Perez, Jessica R.
Ybarra, Norma
Chagnon, Frederic
Serban, Monica
Lee, Sangkyu
Seuntjens, Jan
Lesur, Olivier
El Naqa, Issam
author_sort Perez, Jessica R.
collection PubMed
description Mesenchymal stem cells (MSCs) have potential for reducing inflammation and promoting organ repair. However, limitations in available techniques to track them and assess this potential for lung repair have hindered their applicability. In this work, we proposed, implemented and evaluated the use of fluorescence endomicroscopy as a novel imaging tool to track MSCs in vivo. MSCs were fluorescently labeled and injected into a rat model of radiation-induced lung injury via endotracheal (ET) or intravascular (IV) administration. Our results show that MSCs were visible in the lungs with fluorescence endomicroscopy. Moreover, we developed an automatic cell counting algorithm to quantify the number of detected cells in each condition. We observed a significantly higher number of detected cells in ET injection compared to IV and a slight increase in the mean number of detected cells in irradiated lungs compared to control, although the latter did not reach statistical significance. Fluorescence endomicroscopy imaging is a powerful new minimally invasive and translatable tool that can be used to track and quantify MSCs in the lungs and help assess their potential in organ repair.
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spelling pubmed-52444042017-01-23 Tracking of Mesenchymal Stem Cells with Fluorescence Endomicroscopy Imaging in Radiotherapy-Induced Lung Injury Perez, Jessica R. Ybarra, Norma Chagnon, Frederic Serban, Monica Lee, Sangkyu Seuntjens, Jan Lesur, Olivier El Naqa, Issam Sci Rep Article Mesenchymal stem cells (MSCs) have potential for reducing inflammation and promoting organ repair. However, limitations in available techniques to track them and assess this potential for lung repair have hindered their applicability. In this work, we proposed, implemented and evaluated the use of fluorescence endomicroscopy as a novel imaging tool to track MSCs in vivo. MSCs were fluorescently labeled and injected into a rat model of radiation-induced lung injury via endotracheal (ET) or intravascular (IV) administration. Our results show that MSCs were visible in the lungs with fluorescence endomicroscopy. Moreover, we developed an automatic cell counting algorithm to quantify the number of detected cells in each condition. We observed a significantly higher number of detected cells in ET injection compared to IV and a slight increase in the mean number of detected cells in irradiated lungs compared to control, although the latter did not reach statistical significance. Fluorescence endomicroscopy imaging is a powerful new minimally invasive and translatable tool that can be used to track and quantify MSCs in the lungs and help assess their potential in organ repair. Nature Publishing Group 2017-01-19 /pmc/articles/PMC5244404/ /pubmed/28102237 http://dx.doi.org/10.1038/srep40748 Text en Copyright © 2017, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Perez, Jessica R.
Ybarra, Norma
Chagnon, Frederic
Serban, Monica
Lee, Sangkyu
Seuntjens, Jan
Lesur, Olivier
El Naqa, Issam
Tracking of Mesenchymal Stem Cells with Fluorescence Endomicroscopy Imaging in Radiotherapy-Induced Lung Injury
title Tracking of Mesenchymal Stem Cells with Fluorescence Endomicroscopy Imaging in Radiotherapy-Induced Lung Injury
title_full Tracking of Mesenchymal Stem Cells with Fluorescence Endomicroscopy Imaging in Radiotherapy-Induced Lung Injury
title_fullStr Tracking of Mesenchymal Stem Cells with Fluorescence Endomicroscopy Imaging in Radiotherapy-Induced Lung Injury
title_full_unstemmed Tracking of Mesenchymal Stem Cells with Fluorescence Endomicroscopy Imaging in Radiotherapy-Induced Lung Injury
title_short Tracking of Mesenchymal Stem Cells with Fluorescence Endomicroscopy Imaging in Radiotherapy-Induced Lung Injury
title_sort tracking of mesenchymal stem cells with fluorescence endomicroscopy imaging in radiotherapy-induced lung injury
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5244404/
https://www.ncbi.nlm.nih.gov/pubmed/28102237
http://dx.doi.org/10.1038/srep40748
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