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Time-resolved neutron scattering provides new insight into protein substrate processing by a AAA+ unfoldase
We present a combination of small-angle neutron scattering, deuterium labelling and contrast variation, temperature activation and fluorescence spectroscopy as a novel approach to obtain time-resolved, structural data individually from macromolecular complexes and their substrates during active bioc...
Autores principales: | Ibrahim, Ziad, Martel, Anne, Moulin, Martine, Kim, Henry S., Härtlein, Michael, Franzetti, Bruno, Gabel, Frank |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5244417/ https://www.ncbi.nlm.nih.gov/pubmed/28102317 http://dx.doi.org/10.1038/srep40948 |
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