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The gastro protective effects of Cibotium barometz hair on ethanol-induced gastric ulcer in Sprague-Dawley rats

BACKGROUND: Cibotium barometz is a medical herb used traditionally in the Malaysian peninsula for several ailments, including gastric ulcer. The aim of this study was assessment the anti-ulcer effects of C. barometz hair on ethanol-induced stomach hemorrhagic abrasions in animals. Seven groups of Sp...

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Autores principales: AL-Wajeeh, Nahla Saeed, Hajerezaie, Maryam, Noor, Suzita Mohd, Halabi, Mohammed Farouq, Al-Henhena, Nawal, Azizan, Ainnul Hamidah Syahadah, Kamran, Sareh, Hassandarvish, Pouya, Shwter, Abdrabuh N., karimian, Hamed, Ali, Hapipah Mohd, Abdulla, Mahmood Ameen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5244617/
https://www.ncbi.nlm.nih.gov/pubmed/28103938
http://dx.doi.org/10.1186/s12917-017-0949-z
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author AL-Wajeeh, Nahla Saeed
Hajerezaie, Maryam
Noor, Suzita Mohd
Halabi, Mohammed Farouq
Al-Henhena, Nawal
Azizan, Ainnul Hamidah Syahadah
Kamran, Sareh
Hassandarvish, Pouya
Shwter, Abdrabuh N.
karimian, Hamed
Ali, Hapipah Mohd
Abdulla, Mahmood Ameen
author_facet AL-Wajeeh, Nahla Saeed
Hajerezaie, Maryam
Noor, Suzita Mohd
Halabi, Mohammed Farouq
Al-Henhena, Nawal
Azizan, Ainnul Hamidah Syahadah
Kamran, Sareh
Hassandarvish, Pouya
Shwter, Abdrabuh N.
karimian, Hamed
Ali, Hapipah Mohd
Abdulla, Mahmood Ameen
author_sort AL-Wajeeh, Nahla Saeed
collection PubMed
description BACKGROUND: Cibotium barometz is a medical herb used traditionally in the Malaysian peninsula for several ailments, including gastric ulcer. The aim of this study was assessment the anti-ulcer effects of C. barometz hair on ethanol-induced stomach hemorrhagic abrasions in animals. Seven groups of Sprague Dawley (SD) rats were administered 10% Tween 20 in the normal control and ulcer control groups, and omeprazole 20 mg/kg and 62.5, 125, 250, and 500 mg/kg of C. barometz hair extract in the experimental groups. After 60 min, the normal control group of rats was orally administered 10% Tween 20, while absolute ethanol was orally administered to the groups of ulcer control, omeprazole and experimental groups. Stomachs of the rats were examined macroscopically and histologically. Homogenates of stomachs were used to evaluate endogenous antioxidant enzyme activities. RESULTS: Rats pre-fed with plant extract presented a significant decrease in the sore area, increased pH of gastric contents and preserved stomach wall mucus compared to the ulcer group. Histologically, rats pre-fed with C. barometz hair extract showed mild to moderate disruptions of the surface epithelium while animals pre-fed with absolute ethanol showed severe disruptions of the stomach epithelium with edema and leucocyte penetration of the submucosal layer. A Periodic acid Schiff (PAS) staining revealed that each rat pre-treated with the plant extract displayed an intense uptake of stomach epithelial glycoprotein magenta color compared to the ulcer control group. Immunohistochemical analysis revealed that rats pre-fed with the plant extract showed an up-regulation of the heat shock protein 70 (HSP70) and down-regulation of Bax proteins compared to ulcer control rats. Homogenates of the stomach tissue demonstrated significant increases in the endogenous antioxidant enzymatic activity and decreased lipid peroxidation (MDA) in rats pre-treated with C. barometz hair extract compared with the ulcer control rats. In acute toxicity, the liver and kidney revealed no hepatotoxic or nephrotoxic effects histologically. CONCLUSIONS: The gastric cytoprotective action of C. barometz hair extract might be attributed to antioxidants, an increase in gastric pH, stomach mucus preservation, increased endogenous antioxidant enzymes, decreased lipid peroxidation, up-regulation of HSP70 and down-regulation of Bax proteins.
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spelling pubmed-52446172017-01-23 The gastro protective effects of Cibotium barometz hair on ethanol-induced gastric ulcer in Sprague-Dawley rats AL-Wajeeh, Nahla Saeed Hajerezaie, Maryam Noor, Suzita Mohd Halabi, Mohammed Farouq Al-Henhena, Nawal Azizan, Ainnul Hamidah Syahadah Kamran, Sareh Hassandarvish, Pouya Shwter, Abdrabuh N. karimian, Hamed Ali, Hapipah Mohd Abdulla, Mahmood Ameen BMC Vet Res Research Article BACKGROUND: Cibotium barometz is a medical herb used traditionally in the Malaysian peninsula for several ailments, including gastric ulcer. The aim of this study was assessment the anti-ulcer effects of C. barometz hair on ethanol-induced stomach hemorrhagic abrasions in animals. Seven groups of Sprague Dawley (SD) rats were administered 10% Tween 20 in the normal control and ulcer control groups, and omeprazole 20 mg/kg and 62.5, 125, 250, and 500 mg/kg of C. barometz hair extract in the experimental groups. After 60 min, the normal control group of rats was orally administered 10% Tween 20, while absolute ethanol was orally administered to the groups of ulcer control, omeprazole and experimental groups. Stomachs of the rats were examined macroscopically and histologically. Homogenates of stomachs were used to evaluate endogenous antioxidant enzyme activities. RESULTS: Rats pre-fed with plant extract presented a significant decrease in the sore area, increased pH of gastric contents and preserved stomach wall mucus compared to the ulcer group. Histologically, rats pre-fed with C. barometz hair extract showed mild to moderate disruptions of the surface epithelium while animals pre-fed with absolute ethanol showed severe disruptions of the stomach epithelium with edema and leucocyte penetration of the submucosal layer. A Periodic acid Schiff (PAS) staining revealed that each rat pre-treated with the plant extract displayed an intense uptake of stomach epithelial glycoprotein magenta color compared to the ulcer control group. Immunohistochemical analysis revealed that rats pre-fed with the plant extract showed an up-regulation of the heat shock protein 70 (HSP70) and down-regulation of Bax proteins compared to ulcer control rats. Homogenates of the stomach tissue demonstrated significant increases in the endogenous antioxidant enzymatic activity and decreased lipid peroxidation (MDA) in rats pre-treated with C. barometz hair extract compared with the ulcer control rats. In acute toxicity, the liver and kidney revealed no hepatotoxic or nephrotoxic effects histologically. CONCLUSIONS: The gastric cytoprotective action of C. barometz hair extract might be attributed to antioxidants, an increase in gastric pH, stomach mucus preservation, increased endogenous antioxidant enzymes, decreased lipid peroxidation, up-regulation of HSP70 and down-regulation of Bax proteins. BioMed Central 2017-01-19 /pmc/articles/PMC5244617/ /pubmed/28103938 http://dx.doi.org/10.1186/s12917-017-0949-z Text en © The Author(s). 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
AL-Wajeeh, Nahla Saeed
Hajerezaie, Maryam
Noor, Suzita Mohd
Halabi, Mohammed Farouq
Al-Henhena, Nawal
Azizan, Ainnul Hamidah Syahadah
Kamran, Sareh
Hassandarvish, Pouya
Shwter, Abdrabuh N.
karimian, Hamed
Ali, Hapipah Mohd
Abdulla, Mahmood Ameen
The gastro protective effects of Cibotium barometz hair on ethanol-induced gastric ulcer in Sprague-Dawley rats
title The gastro protective effects of Cibotium barometz hair on ethanol-induced gastric ulcer in Sprague-Dawley rats
title_full The gastro protective effects of Cibotium barometz hair on ethanol-induced gastric ulcer in Sprague-Dawley rats
title_fullStr The gastro protective effects of Cibotium barometz hair on ethanol-induced gastric ulcer in Sprague-Dawley rats
title_full_unstemmed The gastro protective effects of Cibotium barometz hair on ethanol-induced gastric ulcer in Sprague-Dawley rats
title_short The gastro protective effects of Cibotium barometz hair on ethanol-induced gastric ulcer in Sprague-Dawley rats
title_sort gastro protective effects of cibotium barometz hair on ethanol-induced gastric ulcer in sprague-dawley rats
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5244617/
https://www.ncbi.nlm.nih.gov/pubmed/28103938
http://dx.doi.org/10.1186/s12917-017-0949-z
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