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Different routes and doses influence protection in pigs immunised with the naturally attenuated African swine fever virus isolate OURT88/3
This study compares different combinations of doses and routes of immunisation of pigs with low virulent African swine fever virus (ASFV) genotype I isolate OURT88/3, including the intramuscular and intranasal route, the latter not previously tested. Intranasal immunisations with low and moderate do...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5245086/ https://www.ncbi.nlm.nih.gov/pubmed/27908827 http://dx.doi.org/10.1016/j.antiviral.2016.11.021 |
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author | Sánchez-Cordón, Pedro J. Chapman, Dave Jabbar, Tamara Reis, Ana L. Goatley, Lynnette Netherton, Christopher L. Taylor, Geraldine Montoya, Maria Dixon, Linda |
author_facet | Sánchez-Cordón, Pedro J. Chapman, Dave Jabbar, Tamara Reis, Ana L. Goatley, Lynnette Netherton, Christopher L. Taylor, Geraldine Montoya, Maria Dixon, Linda |
author_sort | Sánchez-Cordón, Pedro J. |
collection | PubMed |
description | This study compares different combinations of doses and routes of immunisation of pigs with low virulent African swine fever virus (ASFV) genotype I isolate OURT88/3, including the intramuscular and intranasal route, the latter not previously tested. Intranasal immunisations with low and moderate doses (10(3) and 10(4) TCID(50)) of OURT88/3 provided complete protection (100%) against challenge with virulent genotype I OURT88/1 isolate. Only mild and transient clinical reactions were observed in protected pigs. Transient moderate virus genome levels were detected in blood samples after challenge that decreased, but persisted until the end of the experiment in some animals. In contrast, pigs immunised intramuscularly with low and moderate doses (10(3) and 10(4) TCID(50)) displayed lower percentages of protection (50–66%), and low or undetectable levels of virus genome were detected in blood samples throughout the study. In addition, clinical courses observed in protected pigs were asymptomatic. In pigs that were not protected and developed acute ASF, an exacerbated increase of IL-10 sometimes accompanied by an increase of IFNγ was observed before euthanasia. These results showed that factors including delivery route and dose determine the outcome of immunisation with the naturally attenuated isolate OURT88/3. |
format | Online Article Text |
id | pubmed-5245086 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-52450862017-02-01 Different routes and doses influence protection in pigs immunised with the naturally attenuated African swine fever virus isolate OURT88/3 Sánchez-Cordón, Pedro J. Chapman, Dave Jabbar, Tamara Reis, Ana L. Goatley, Lynnette Netherton, Christopher L. Taylor, Geraldine Montoya, Maria Dixon, Linda Antiviral Res Article This study compares different combinations of doses and routes of immunisation of pigs with low virulent African swine fever virus (ASFV) genotype I isolate OURT88/3, including the intramuscular and intranasal route, the latter not previously tested. Intranasal immunisations with low and moderate doses (10(3) and 10(4) TCID(50)) of OURT88/3 provided complete protection (100%) against challenge with virulent genotype I OURT88/1 isolate. Only mild and transient clinical reactions were observed in protected pigs. Transient moderate virus genome levels were detected in blood samples after challenge that decreased, but persisted until the end of the experiment in some animals. In contrast, pigs immunised intramuscularly with low and moderate doses (10(3) and 10(4) TCID(50)) displayed lower percentages of protection (50–66%), and low or undetectable levels of virus genome were detected in blood samples throughout the study. In addition, clinical courses observed in protected pigs were asymptomatic. In pigs that were not protected and developed acute ASF, an exacerbated increase of IL-10 sometimes accompanied by an increase of IFNγ was observed before euthanasia. These results showed that factors including delivery route and dose determine the outcome of immunisation with the naturally attenuated isolate OURT88/3. Elsevier 2017-02 /pmc/articles/PMC5245086/ /pubmed/27908827 http://dx.doi.org/10.1016/j.antiviral.2016.11.021 Text en © 2016 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Sánchez-Cordón, Pedro J. Chapman, Dave Jabbar, Tamara Reis, Ana L. Goatley, Lynnette Netherton, Christopher L. Taylor, Geraldine Montoya, Maria Dixon, Linda Different routes and doses influence protection in pigs immunised with the naturally attenuated African swine fever virus isolate OURT88/3 |
title | Different routes and doses influence protection in pigs immunised with the naturally attenuated African swine fever virus isolate OURT88/3 |
title_full | Different routes and doses influence protection in pigs immunised with the naturally attenuated African swine fever virus isolate OURT88/3 |
title_fullStr | Different routes and doses influence protection in pigs immunised with the naturally attenuated African swine fever virus isolate OURT88/3 |
title_full_unstemmed | Different routes and doses influence protection in pigs immunised with the naturally attenuated African swine fever virus isolate OURT88/3 |
title_short | Different routes and doses influence protection in pigs immunised with the naturally attenuated African swine fever virus isolate OURT88/3 |
title_sort | different routes and doses influence protection in pigs immunised with the naturally attenuated african swine fever virus isolate ourt88/3 |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5245086/ https://www.ncbi.nlm.nih.gov/pubmed/27908827 http://dx.doi.org/10.1016/j.antiviral.2016.11.021 |
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