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Levodopa attenuates cellular apoptosis in steroid-associated necrosis of the femoral head

The present study aimed to investigate the effects of levodopa (LEV) on cellular apoptosis in a rabbit model of steroid-associated necrosis of the femoral head (SANFH). A total of 44 healthy adult Chinese rabbits were randomly divided into three groups: Group A (n=15), administered a combination of...

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Autores principales: Xi, Hongbo, Tao, Weijian, Jian, Zhengguang, Sun, Xuefeng, Gong, Xiaohong, Huang, Lixin, Dong, Tianhua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5245153/
https://www.ncbi.nlm.nih.gov/pubmed/28123470
http://dx.doi.org/10.3892/etm.2016.3964
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author Xi, Hongbo
Tao, Weijian
Jian, Zhengguang
Sun, Xuefeng
Gong, Xiaohong
Huang, Lixin
Dong, Tianhua
author_facet Xi, Hongbo
Tao, Weijian
Jian, Zhengguang
Sun, Xuefeng
Gong, Xiaohong
Huang, Lixin
Dong, Tianhua
author_sort Xi, Hongbo
collection PubMed
description The present study aimed to investigate the effects of levodopa (LEV) on cellular apoptosis in a rabbit model of steroid-associated necrosis of the femoral head (SANFH). A total of 44 healthy adult Chinese rabbits were randomly divided into three groups: Group A (n=15), administered a combination of lipopolysaccharide and hormone to establish the SANFH animal model; group B (n=15), SANFH animal model as in group A orally administered LEV (0.4 g/kg/day) on the day of injection; and group C (n=14), the control group. On the 6th and 8th week of modeling, seven rabbits from each group were sacrificed to harvest bilateral femoral head specimens for hematoxylin and eosin staining and apoptosis detection by terminal deoxynucleotidyl transferase dUTP nick-end labeling assay analysis, as well as for observing pathological changes and analyzing cellular apoptosis. Eight weeks after modeling, the serum insulin-like growth factor (IGF)-1 levels of the three groups were measured. The empty lacunae rate and apoptosis index of bone cells in the treatment group were significantly lower than that of the model group (P<0.01). Eight weeks after treatment, the serum levels of IGF-1 were significantly higher than that of the model group (P<0.01). These findings suggested that LEV was able to reduce steroid-induced bone cellular apoptosis, reduce the occurrence of necrosis of the femoral head and, through in vivo metabolism, it may promote the synthesis and release of IGF-1, which could be one of its biological pathways to prevent and treat SANFH.
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spelling pubmed-52451532017-01-25 Levodopa attenuates cellular apoptosis in steroid-associated necrosis of the femoral head Xi, Hongbo Tao, Weijian Jian, Zhengguang Sun, Xuefeng Gong, Xiaohong Huang, Lixin Dong, Tianhua Exp Ther Med Articles The present study aimed to investigate the effects of levodopa (LEV) on cellular apoptosis in a rabbit model of steroid-associated necrosis of the femoral head (SANFH). A total of 44 healthy adult Chinese rabbits were randomly divided into three groups: Group A (n=15), administered a combination of lipopolysaccharide and hormone to establish the SANFH animal model; group B (n=15), SANFH animal model as in group A orally administered LEV (0.4 g/kg/day) on the day of injection; and group C (n=14), the control group. On the 6th and 8th week of modeling, seven rabbits from each group were sacrificed to harvest bilateral femoral head specimens for hematoxylin and eosin staining and apoptosis detection by terminal deoxynucleotidyl transferase dUTP nick-end labeling assay analysis, as well as for observing pathological changes and analyzing cellular apoptosis. Eight weeks after modeling, the serum insulin-like growth factor (IGF)-1 levels of the three groups were measured. The empty lacunae rate and apoptosis index of bone cells in the treatment group were significantly lower than that of the model group (P<0.01). Eight weeks after treatment, the serum levels of IGF-1 were significantly higher than that of the model group (P<0.01). These findings suggested that LEV was able to reduce steroid-induced bone cellular apoptosis, reduce the occurrence of necrosis of the femoral head and, through in vivo metabolism, it may promote the synthesis and release of IGF-1, which could be one of its biological pathways to prevent and treat SANFH. D.A. Spandidos 2017-01 2016-12-07 /pmc/articles/PMC5245153/ /pubmed/28123470 http://dx.doi.org/10.3892/etm.2016.3964 Text en Copyright: © Xi et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Xi, Hongbo
Tao, Weijian
Jian, Zhengguang
Sun, Xuefeng
Gong, Xiaohong
Huang, Lixin
Dong, Tianhua
Levodopa attenuates cellular apoptosis in steroid-associated necrosis of the femoral head
title Levodopa attenuates cellular apoptosis in steroid-associated necrosis of the femoral head
title_full Levodopa attenuates cellular apoptosis in steroid-associated necrosis of the femoral head
title_fullStr Levodopa attenuates cellular apoptosis in steroid-associated necrosis of the femoral head
title_full_unstemmed Levodopa attenuates cellular apoptosis in steroid-associated necrosis of the femoral head
title_short Levodopa attenuates cellular apoptosis in steroid-associated necrosis of the femoral head
title_sort levodopa attenuates cellular apoptosis in steroid-associated necrosis of the femoral head
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5245153/
https://www.ncbi.nlm.nih.gov/pubmed/28123470
http://dx.doi.org/10.3892/etm.2016.3964
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