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Cellular Interactions and Formation of an Epithelial “Nanocoating-Like Barrier” with Mesoporous Silica Nanoparticles

Oral mucosa as the front-line barrier in the mouth is constantly exposed to a complex microenvironment with multitudinous microbes. In this study, the interactions of mesoporous silica nanoparticles (MSNs) with primary human gingival epithelial cells were analyzed for up to 72 h, and their diffusion...

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Autores principales: Li, Xuan, Pang, Ka Yan, Ng, Tsz Wing, Leung, Ping Chung, Zhang, Cheng Fei, Leung, Ken Cham-Fai, Jin, Lijian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5245761/
https://www.ncbi.nlm.nih.gov/pubmed/28335320
http://dx.doi.org/10.3390/nano6110192
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author Li, Xuan
Pang, Ka Yan
Ng, Tsz Wing
Leung, Ping Chung
Zhang, Cheng Fei
Leung, Ken Cham-Fai
Jin, Lijian
author_facet Li, Xuan
Pang, Ka Yan
Ng, Tsz Wing
Leung, Ping Chung
Zhang, Cheng Fei
Leung, Ken Cham-Fai
Jin, Lijian
author_sort Li, Xuan
collection PubMed
description Oral mucosa as the front-line barrier in the mouth is constantly exposed to a complex microenvironment with multitudinous microbes. In this study, the interactions of mesoporous silica nanoparticles (MSNs) with primary human gingival epithelial cells were analyzed for up to 72 h, and their diffusion capacity in the reconstructed human gingival epithelia (RHGE) and porcine ear skin models was further assessed at 24 h. It was found that the synthesized fluorescent mesoporous silica nanoparticles (RITC-NPs) with low cytotoxicity could be uptaken, degraded, and/or excreted by the human gingival epithelial cells. Moreover, the RITC-NPs penetrated into the stratum corneum of RHGE in a time-dependent manner, while they were unable to get across the barrier of stratum corneum in the porcine ear skins. Consequently, the penetration and accumulation of RITC-NPs at the corneum layers of epithelia could form a “nanocoating-like barrier”. This preliminary proof-of-concept study suggests the feasibility of developing nanoparticle-based antimicrobial and anti-inflammatory agents through topical application for oral healthcare.
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spelling pubmed-52457612017-03-21 Cellular Interactions and Formation of an Epithelial “Nanocoating-Like Barrier” with Mesoporous Silica Nanoparticles Li, Xuan Pang, Ka Yan Ng, Tsz Wing Leung, Ping Chung Zhang, Cheng Fei Leung, Ken Cham-Fai Jin, Lijian Nanomaterials (Basel) Communication Oral mucosa as the front-line barrier in the mouth is constantly exposed to a complex microenvironment with multitudinous microbes. In this study, the interactions of mesoporous silica nanoparticles (MSNs) with primary human gingival epithelial cells were analyzed for up to 72 h, and their diffusion capacity in the reconstructed human gingival epithelia (RHGE) and porcine ear skin models was further assessed at 24 h. It was found that the synthesized fluorescent mesoporous silica nanoparticles (RITC-NPs) with low cytotoxicity could be uptaken, degraded, and/or excreted by the human gingival epithelial cells. Moreover, the RITC-NPs penetrated into the stratum corneum of RHGE in a time-dependent manner, while they were unable to get across the barrier of stratum corneum in the porcine ear skins. Consequently, the penetration and accumulation of RITC-NPs at the corneum layers of epithelia could form a “nanocoating-like barrier”. This preliminary proof-of-concept study suggests the feasibility of developing nanoparticle-based antimicrobial and anti-inflammatory agents through topical application for oral healthcare. MDPI 2016-10-27 /pmc/articles/PMC5245761/ /pubmed/28335320 http://dx.doi.org/10.3390/nano6110192 Text en © 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Li, Xuan
Pang, Ka Yan
Ng, Tsz Wing
Leung, Ping Chung
Zhang, Cheng Fei
Leung, Ken Cham-Fai
Jin, Lijian
Cellular Interactions and Formation of an Epithelial “Nanocoating-Like Barrier” with Mesoporous Silica Nanoparticles
title Cellular Interactions and Formation of an Epithelial “Nanocoating-Like Barrier” with Mesoporous Silica Nanoparticles
title_full Cellular Interactions and Formation of an Epithelial “Nanocoating-Like Barrier” with Mesoporous Silica Nanoparticles
title_fullStr Cellular Interactions and Formation of an Epithelial “Nanocoating-Like Barrier” with Mesoporous Silica Nanoparticles
title_full_unstemmed Cellular Interactions and Formation of an Epithelial “Nanocoating-Like Barrier” with Mesoporous Silica Nanoparticles
title_short Cellular Interactions and Formation of an Epithelial “Nanocoating-Like Barrier” with Mesoporous Silica Nanoparticles
title_sort cellular interactions and formation of an epithelial “nanocoating-like barrier” with mesoporous silica nanoparticles
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5245761/
https://www.ncbi.nlm.nih.gov/pubmed/28335320
http://dx.doi.org/10.3390/nano6110192
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