Active Hydrogen Bond Network (AHBN) and Applications for Improvement of Thermal Stability and pH-Sensitivity of Pullulanase from Bacillus naganoensis

A method, so called “active hydrogen bond network” (AHBN), is proposed for site-directed mutations of hydrolytic enzymes. In an enzyme the AHBN consists of the active residues, functional residues, and conservative water molecules, which are connected by hydrogen bonds, forming a three dimensional n...

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Detalles Bibliográficos
Autores principales: Wang, Qing-Yan, Xie, Neng-Zhong, Du, Qi-Shi, Qin, Yan, Li, Jian-Xiu, Meng, Jian-Zong, Huang, Ri-Bo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5245800/
https://www.ncbi.nlm.nih.gov/pubmed/28103251
http://dx.doi.org/10.1371/journal.pone.0169080

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5245800/
https://www.ncbi.nlm.nih.gov/pubmed/28103251
http://dx.doi.org/10.1371/journal.pone.0169080

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