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Association between telomere length and CYP19 TTTA repetition polymorphism in healthy and breast cancer-diagnosed women

INTRODUCTION: Several studies have reported an increase in breast cancer (BC) risk when patients are carriers of the CYP19 TTA polymorphism with ≥10 repeats; moreover, it has been reported that telomere length is associated with a higher susceptibility of developing cancer. OBJECTIVE: The objective...

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Autores principales: Murillo-Ortiz, Blanca, Martínez-Garza, Sandra, Suárez García, David, Castillo Valenzuela, Rosa del Carmen, García Regalado, Juan Francisco, Cano Velázquez, Gerardo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove Medical Press 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5245810/
https://www.ncbi.nlm.nih.gov/pubmed/28144163
http://dx.doi.org/10.2147/BCTT.S125431
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author Murillo-Ortiz, Blanca
Martínez-Garza, Sandra
Suárez García, David
Castillo Valenzuela, Rosa del Carmen
García Regalado, Juan Francisco
Cano Velázquez, Gerardo
author_facet Murillo-Ortiz, Blanca
Martínez-Garza, Sandra
Suárez García, David
Castillo Valenzuela, Rosa del Carmen
García Regalado, Juan Francisco
Cano Velázquez, Gerardo
author_sort Murillo-Ortiz, Blanca
collection PubMed
description INTRODUCTION: Several studies have reported an increase in breast cancer (BC) risk when patients are carriers of the CYP19 TTA polymorphism with ≥10 repeats; moreover, it has been reported that telomere length is associated with a higher susceptibility of developing cancer. OBJECTIVE: The objective of this study was to understand the relationship between CYP19 TTTA repetition polymorphism and telomere length and its effects on serum estradiol, estrone and follicle-stimulating hormone (FSH). MATERIALS AND METHODS: A total of 180 postmenopausal healthy and 70 BC-diagnosed women were included. Telomere length was determined through real-time quantitative polymerase chain reaction, and aromatase polymorphism was analyzed through DNA; both samples were obtained from circulating leukocytes. Serum estrone, estradiol and FSH were determined by enzyme-linked immunosorbent assay. RESULTS: Patients with a BC diagnosis showed >10 repetitions more frequently, compared with that of healthy women (50% vs 23%, χ(2) = 11.44, p = 0.0007). A significant difference in telomere length between healthy and BC women was observed (5,042.7 vs 2,256.7 pb, Z = 4.88, p < 0.001). CYP19 TTTA repeat polymorphism was associated with serum levels of estradiol and estrone in both groups, being higher in those with >10 repeats. Moreover, telomere length showed an inverse relationship with the number of repeats of the aromatase polymorphism in healthy women (R(2) = 0.04, r = −0.24); in contrast, BC patients did not display this relationship. In addition, telomere length presented an inverse relationship with serum levels of estradiol and estrone in BC patients (p = 0.02). CONCLUSION: Telomere length is shorter in BC patients than in healthy patients. The CYP19 TTTA repeat polymorphism is associated with serum levels of estradiol and estrone in both healthy women and BC patients, being higher in those with polymorphism carriers >10 repeats. Telomere length has an inverse correlation with the number of repeats of the aromatase polymorphism in healthy women but not in BC women. Estradiol and estrone levels in BC women have an inverse relationship with telomere length.
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spelling pubmed-52458102017-01-31 Association between telomere length and CYP19 TTTA repetition polymorphism in healthy and breast cancer-diagnosed women Murillo-Ortiz, Blanca Martínez-Garza, Sandra Suárez García, David Castillo Valenzuela, Rosa del Carmen García Regalado, Juan Francisco Cano Velázquez, Gerardo Breast Cancer (Dove Med Press) Original Research INTRODUCTION: Several studies have reported an increase in breast cancer (BC) risk when patients are carriers of the CYP19 TTA polymorphism with ≥10 repeats; moreover, it has been reported that telomere length is associated with a higher susceptibility of developing cancer. OBJECTIVE: The objective of this study was to understand the relationship between CYP19 TTTA repetition polymorphism and telomere length and its effects on serum estradiol, estrone and follicle-stimulating hormone (FSH). MATERIALS AND METHODS: A total of 180 postmenopausal healthy and 70 BC-diagnosed women were included. Telomere length was determined through real-time quantitative polymerase chain reaction, and aromatase polymorphism was analyzed through DNA; both samples were obtained from circulating leukocytes. Serum estrone, estradiol and FSH were determined by enzyme-linked immunosorbent assay. RESULTS: Patients with a BC diagnosis showed >10 repetitions more frequently, compared with that of healthy women (50% vs 23%, χ(2) = 11.44, p = 0.0007). A significant difference in telomere length between healthy and BC women was observed (5,042.7 vs 2,256.7 pb, Z = 4.88, p < 0.001). CYP19 TTTA repeat polymorphism was associated with serum levels of estradiol and estrone in both groups, being higher in those with >10 repeats. Moreover, telomere length showed an inverse relationship with the number of repeats of the aromatase polymorphism in healthy women (R(2) = 0.04, r = −0.24); in contrast, BC patients did not display this relationship. In addition, telomere length presented an inverse relationship with serum levels of estradiol and estrone in BC patients (p = 0.02). CONCLUSION: Telomere length is shorter in BC patients than in healthy patients. The CYP19 TTTA repeat polymorphism is associated with serum levels of estradiol and estrone in both healthy women and BC patients, being higher in those with polymorphism carriers >10 repeats. Telomere length has an inverse correlation with the number of repeats of the aromatase polymorphism in healthy women but not in BC women. Estradiol and estrone levels in BC women have an inverse relationship with telomere length. Dove Medical Press 2017-01-13 /pmc/articles/PMC5245810/ /pubmed/28144163 http://dx.doi.org/10.2147/BCTT.S125431 Text en © 2017 Murillo-Ortiz et al. This work is published and licensed by Dove Medical Press Limited The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.
spellingShingle Original Research
Murillo-Ortiz, Blanca
Martínez-Garza, Sandra
Suárez García, David
Castillo Valenzuela, Rosa del Carmen
García Regalado, Juan Francisco
Cano Velázquez, Gerardo
Association between telomere length and CYP19 TTTA repetition polymorphism in healthy and breast cancer-diagnosed women
title Association between telomere length and CYP19 TTTA repetition polymorphism in healthy and breast cancer-diagnosed women
title_full Association between telomere length and CYP19 TTTA repetition polymorphism in healthy and breast cancer-diagnosed women
title_fullStr Association between telomere length and CYP19 TTTA repetition polymorphism in healthy and breast cancer-diagnosed women
title_full_unstemmed Association between telomere length and CYP19 TTTA repetition polymorphism in healthy and breast cancer-diagnosed women
title_short Association between telomere length and CYP19 TTTA repetition polymorphism in healthy and breast cancer-diagnosed women
title_sort association between telomere length and cyp19 ttta repetition polymorphism in healthy and breast cancer-diagnosed women
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5245810/
https://www.ncbi.nlm.nih.gov/pubmed/28144163
http://dx.doi.org/10.2147/BCTT.S125431
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