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The Antiviral RNAi Response in Vector and Non-vector Cells against Orthobunyaviruses
BACKGROUND: Vector arthropods control arbovirus replication and spread through antiviral innate immune responses including RNA interference (RNAi) pathways. Arbovirus infections have been shown to induce the exogenous small interfering RNA (siRNA) and Piwi-interacting RNA (piRNA) pathways, but direc...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5245901/ https://www.ncbi.nlm.nih.gov/pubmed/28060823 http://dx.doi.org/10.1371/journal.pntd.0005272 |
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author | Dietrich, Isabelle Shi, Xiaohong McFarlane, Melanie Watson, Mick Blomström, Anne-Lie Skelton, Jessica K. Kohl, Alain Elliott, Richard M. Schnettler, Esther |
author_facet | Dietrich, Isabelle Shi, Xiaohong McFarlane, Melanie Watson, Mick Blomström, Anne-Lie Skelton, Jessica K. Kohl, Alain Elliott, Richard M. Schnettler, Esther |
author_sort | Dietrich, Isabelle |
collection | PubMed |
description | BACKGROUND: Vector arthropods control arbovirus replication and spread through antiviral innate immune responses including RNA interference (RNAi) pathways. Arbovirus infections have been shown to induce the exogenous small interfering RNA (siRNA) and Piwi-interacting RNA (piRNA) pathways, but direct antiviral activity by these host responses in mosquito cells has only been demonstrated against a limited number of positive-strand RNA arboviruses. For bunyaviruses in general, the relative contribution of small RNA pathways in antiviral defences is unknown. METHODOLOGY/PRINCIPAL FINDINGS: The genus Orthobunyavirus in the Bunyaviridae family harbours a diverse range of mosquito-, midge- and tick-borne arboviruses. We hypothesized that differences in the antiviral RNAi response in vector versus non-vector cells may exist and that could influence viral host range. Using Aedes aegypti-derived mosquito cells, mosquito-borne orthobunyaviruses and midge-borne orthobunyaviruses we showed that bunyavirus infection commonly induced the production of small RNAs and the effects of the small RNA pathways on individual viruses differ in specific vector-arbovirus interactions. CONCLUSIONS/SIGNIFICANCE: These findings have important implications for our understanding of antiviral RNAi pathways and orthobunyavirus-vector interactions and tropism. |
format | Online Article Text |
id | pubmed-5245901 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-52459012017-02-17 The Antiviral RNAi Response in Vector and Non-vector Cells against Orthobunyaviruses Dietrich, Isabelle Shi, Xiaohong McFarlane, Melanie Watson, Mick Blomström, Anne-Lie Skelton, Jessica K. Kohl, Alain Elliott, Richard M. Schnettler, Esther PLoS Negl Trop Dis Research Article BACKGROUND: Vector arthropods control arbovirus replication and spread through antiviral innate immune responses including RNA interference (RNAi) pathways. Arbovirus infections have been shown to induce the exogenous small interfering RNA (siRNA) and Piwi-interacting RNA (piRNA) pathways, but direct antiviral activity by these host responses in mosquito cells has only been demonstrated against a limited number of positive-strand RNA arboviruses. For bunyaviruses in general, the relative contribution of small RNA pathways in antiviral defences is unknown. METHODOLOGY/PRINCIPAL FINDINGS: The genus Orthobunyavirus in the Bunyaviridae family harbours a diverse range of mosquito-, midge- and tick-borne arboviruses. We hypothesized that differences in the antiviral RNAi response in vector versus non-vector cells may exist and that could influence viral host range. Using Aedes aegypti-derived mosquito cells, mosquito-borne orthobunyaviruses and midge-borne orthobunyaviruses we showed that bunyavirus infection commonly induced the production of small RNAs and the effects of the small RNA pathways on individual viruses differ in specific vector-arbovirus interactions. CONCLUSIONS/SIGNIFICANCE: These findings have important implications for our understanding of antiviral RNAi pathways and orthobunyavirus-vector interactions and tropism. Public Library of Science 2017-01-06 /pmc/articles/PMC5245901/ /pubmed/28060823 http://dx.doi.org/10.1371/journal.pntd.0005272 Text en © 2017 Dietrich et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Dietrich, Isabelle Shi, Xiaohong McFarlane, Melanie Watson, Mick Blomström, Anne-Lie Skelton, Jessica K. Kohl, Alain Elliott, Richard M. Schnettler, Esther The Antiviral RNAi Response in Vector and Non-vector Cells against Orthobunyaviruses |
title | The Antiviral RNAi Response in Vector and Non-vector Cells against Orthobunyaviruses |
title_full | The Antiviral RNAi Response in Vector and Non-vector Cells against Orthobunyaviruses |
title_fullStr | The Antiviral RNAi Response in Vector and Non-vector Cells against Orthobunyaviruses |
title_full_unstemmed | The Antiviral RNAi Response in Vector and Non-vector Cells against Orthobunyaviruses |
title_short | The Antiviral RNAi Response in Vector and Non-vector Cells against Orthobunyaviruses |
title_sort | antiviral rnai response in vector and non-vector cells against orthobunyaviruses |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5245901/ https://www.ncbi.nlm.nih.gov/pubmed/28060823 http://dx.doi.org/10.1371/journal.pntd.0005272 |
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