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A microfluorometric method for quantifying RNA and DNA in terrestrial insects

Evidence is accumulating for a mechanistic linkage between body phosphorus content and growth and reproduction of individual organisms, due in part to variation in allocation of resources to ribosomal RNA. Testing this connection requires reliable methods of quantifying the nucleic acid content of i...

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Detalles Bibliográficos
Autores principales: Kyle, M., Watts, T., Schade, J., Elser, J.J.
Formato: Texto
Lenguaje:English
Publicado: University of Arizona Library 2003
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC524641/
https://www.ncbi.nlm.nih.gov/pubmed/15841218
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author Kyle, M.
Watts, T.
Schade, J.
Elser, J.J.
author_facet Kyle, M.
Watts, T.
Schade, J.
Elser, J.J.
author_sort Kyle, M.
collection PubMed
description Evidence is accumulating for a mechanistic linkage between body phosphorus content and growth and reproduction of individual organisms, due in part to variation in allocation of resources to ribosomal RNA. Testing this connection requires reliable methods of quantifying the nucleic acid content of individual organisms. Although methods for quantifying nucleic acids are available for a wide array of organisms, adaptation of such methods for study of insects has been neglected. Sensitive stains and high throughput fluorometric measurements are now available that substantially improve past methodologies. Here we present methods for the extraction and quantification of insect RNA and DNA based on the use of N-lauroylsarcosine and sonication for extraction, the nucleases RNase and DNase, and the use of microplate fluorescent assays to quantify nucleic acids as percent of body weight in insects. We illustrate the method using Drosophila and curculionid weevils.
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spelling pubmed-5246412005-03-28 A microfluorometric method for quantifying RNA and DNA in terrestrial insects Kyle, M. Watts, T. Schade, J. Elser, J.J. J Insect Sci Articles Evidence is accumulating for a mechanistic linkage between body phosphorus content and growth and reproduction of individual organisms, due in part to variation in allocation of resources to ribosomal RNA. Testing this connection requires reliable methods of quantifying the nucleic acid content of individual organisms. Although methods for quantifying nucleic acids are available for a wide array of organisms, adaptation of such methods for study of insects has been neglected. Sensitive stains and high throughput fluorometric measurements are now available that substantially improve past methodologies. Here we present methods for the extraction and quantification of insect RNA and DNA based on the use of N-lauroylsarcosine and sonication for extraction, the nucleases RNase and DNase, and the use of microplate fluorescent assays to quantify nucleic acids as percent of body weight in insects. We illustrate the method using Drosophila and curculionid weevils. University of Arizona Library 2003-01-07 /pmc/articles/PMC524641/ /pubmed/15841218 Text en Copyright © 2003. Open access; copyright is maintained by the authors.
spellingShingle Articles
Kyle, M.
Watts, T.
Schade, J.
Elser, J.J.
A microfluorometric method for quantifying RNA and DNA in terrestrial insects
title A microfluorometric method for quantifying RNA and DNA in terrestrial insects
title_full A microfluorometric method for quantifying RNA and DNA in terrestrial insects
title_fullStr A microfluorometric method for quantifying RNA and DNA in terrestrial insects
title_full_unstemmed A microfluorometric method for quantifying RNA and DNA in terrestrial insects
title_short A microfluorometric method for quantifying RNA and DNA in terrestrial insects
title_sort microfluorometric method for quantifying rna and dna in terrestrial insects
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC524641/
https://www.ncbi.nlm.nih.gov/pubmed/15841218
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